GPX4 Antibody | Affinity Biosciences

Product:	GPX4 Antibody
Catalog:	DF6701
Description:	Rabbit polyclonal antibody to GPX4
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Chicken
Mol.Wt.:	22kDa; 22kD(Calculated).
Uniprot:	P36969
RRID:	AB_2838663

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(90%), Bovine(100%), Chicken(80%)

Clonality:

Polyclonal

Specificity:

GPX4 Antibody detects endogenous levels of total GPX4.

RRID:

AB_2838663
Cite Format: Affinity Biosciences Cat# DF6701, RRID:AB_2838663.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Glutathione peroxidase 4; GPX 4; GPX-4; GPX4; GPX4_HUMAN; GSHPx-4; MCSP; mitochondrial; PHGPx; Phospholipid hydroperoxidase; Phospholipid hydroperoxide glutathione peroxidase; Phospholipid hydroperoxide glutathione peroxidase mitochondrial; snGPx; snPHGPx; Sperm nucleus glutathione peroxidase;

Immunogens

Immunogen:

A synthesized peptide derived from human GPX4, corresponding to a region within C-terminal amino acids.

Uniprot:

P36969(GPX4_HUMAN) >>Visit HPA database.

Gene(ID):

GPX4(2879)

Expression:

P36969 GPX4_HUMAN:

Present primarily in testis.

Description:

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. [provided by RefSeq, Jul 2008]

Sequence:

P36969 GPX4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLGRLCRLLKPALLCGALAAPGLAGTMCASRDDWRCARSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQUGKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

100

Pig

Chicken

Horse

Sheep

Dog

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36969 As Substrate

P36969

Site	PTM Type	Source
S40	Phosphorylation	Uniprot
K47	Acetylation	Uniprot
K107	Ubiquitination	Uniprot
K162	Ubiquitination	Uniprot
K167	Ubiquitination	Uniprot

Research Backgrounds

P36969_GPX4_HUMAN

Function:

Essential antioxidant peroxidase that directly reduces phospholipid hydroperoxide even if they are incorporated in membranes and lipoproteins (By similarity). Can also reduce fatty acid hydroperoxide, cholesterol hydroperoxide and thymine hydroperoxide (By similarity). Plays a key role in protecting cells from oxidative damage by preventing membrane lipid peroxidation (By similarity). Required to prevent cells from ferroptosis, a non-apoptotic cell death resulting from an iron-dependent accumulation of lipid reactive oxygen species. The presence of selenocysteine (Sec) versus Cys at the active site is essential for life: it provides resistance to overoxidation and prevents cells against ferroptosis (By similarity). The presence of Sec at the active site is also essential for the survival of a specific type of parvalbumin-positive interneurons, thereby preventing against fatal epileptic seizures (By similarity). May be required to protect cells from the toxicity of ingested lipid hydroperoxides (By similarity). Required for normal sperm development and male fertility (By similarity). Essential for maturation and survival of photoreceptor cells (By similarity). Plays a role in a primary T-cell response to viral and parasitic infection by protecting T-cells from ferroptosis and by supporting T-cell expansion (By similarity).

Subcellular Location:

Mitochondrion.

Cytoplasm.

Tissue Specificity:

Present primarily in testis.

Subunit Structure:

Monomer. Has a tendency to form higher mass oligomers.

Family&Domains:

Belongs to the glutathione peroxidase family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

References

1). Cell Membrane Camouflaged Metal Oxide–Black Phosphorus Biomimetic Nanocomplex Enhances Photo-chemo-dynamic Ferroptosis. ACS Applied Materials & Interfaces (PubMed: 35658416) [IF=9.5]

2). Biochanin A protects against iron overload associated knee osteoarthritis via regulating iron levels and NRF2/System xc-/GPX4 axis. Biomedicine & Pharmacotherapy (PubMed: 36379122) [IF=7.5]

3). GSK-3β-dependent Nrf2 antioxidant response modulates ferroptosis of lens epithelial cells in age-related cataract. Free Radical Biology and Medicine (PubMed: 37156294) [IF=7.4]

4). Cigarette smoke induces the ROS accumulation and iNOS activation through deactivation of Nrf-2/SIRT3 axis to mediate the human bronchial epithelium ferroptosis. Free Radical Biology and Medicine (PubMed: 36871899) [IF=7.4]

5). YAP1 alleviates sepsis-induced acute lung injury via inhibiting ferritinophagy-mediated ferroptosis. Frontiers in Immunology (PubMed: 35979359) [IF=7.3]

6). Astragaloside IV attenuates myocardial dysfunction in diabetic cardiomyopathy rats through downregulation of CD36-mediated ferroptosis. Phytotherapy Research (PubMed: 36882189) [IF=7.2]

7). Ferroptosis, a new target for treatment of renal injury and fibrosis in a 5/6 nephrectomy-induced CKD rat model. Cell Death Discovery (PubMed: 35318301) [IF=7.0]

Application: WB Species: Rat Sample:

Fig. 2 Typical features of ferroptosis present in CKD rats. Biochemical trends (a–f) and pathological evidence (g–j) of ferroptosis in CKD rats. Green arrow, normal mitochondria; red arrow, MOM rupture; blue arrow, reduction or loss of MC; yellow arrow, MC swelling. **P < 0.01, ***P < 0.001 vs. Sham group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. CKD group. Scale bar in (a) = 50 μm. Scale bar in (f) = 500 μm.

Application: IF/ICC Species: Rat Sample:

8). Chlorogenic Acid Alleviates Chronic Stress-Induced Duodenal Ferroptosis via the Inhibition of the IL-6/JAK2/STAT3 Signaling Pathway in Rats. Journal of Agricultural and Food Chemistry (PubMed: 35380825) [IF=6.1]

9). Chlorogenic acid alleviates hypoxic-ischemic brain injury in neonatal mice. Neural Regeneration Research (PubMed: 36018179) [IF=6.1]

Application: WB Species: Mouse Sample: brain tissues

Figure 4 CGA improves hypoxic-ischemic brain damage by reducing oxidative stress.(A) The MDA level in brain tissues. (B, C) Western blot of 4-HNE and GPX4 in brain tissues. β-Actin was used as an internal reference. (D, E) Quantitative analyses of 4-HNE and GPX4 (normalized to β-actin). Data are presented as mean ± SEM (n = 4). **P < 0.01, vs. Sham + NS group; #P < 0.05, ##P < 0.01, vs. HI + NS group (one-way analysis of variance followed by Tukey’s post hoc test). 4-HNE: 4-Hydroxynonenal; CGA: chlorogenic acid; GPX4: glutathione peroxidase 4; HI: hypoxic-ischemic brain injury; MDA: malondialdehyde; NS: normal saline.

10). Myo-Inositol Supplementation Alleviates Cisplatin-Induced Acute Kidney Injury via Inhibition of Ferroptosis. Cells (PubMed: 36611810) [IF=6.0]

Application: WB Species: Mouse Sample:

Figure 5. Myo-inositol alleviated ferroptosis in cisplatin-induced AKI in mice. (Panels (A–C)) DHE staining showed that myo-inositol alleviates cisplatin-induced ROS generation in renal tubules. (Panels (D–F)) Immunofluorescence staining of 4-HNE revealed that the increased lipid peroxidation induced by cisplatin was attenuated by myo-inositol. (Panels (G–L)) Immunohistochemistry analyses demonstrated that CHIP was down-regulated while NOX4 was up-regulated in cisplatin-treated kidneys, and their expression was partially restored by myo-inositol treatment. (Panel (M)) Immunoblot analyses revealed that cisplatin treatment led to decreased expression of NCOA4, GPX4, and CHIP, while the expression of FTH1 and NOX4 increased. These changes were largely normalized with the supplementation of myo-inositol. (Panels (N,O)) qRT-PCR analysis showed that the mRNA levels of ACSL4 and PTGS2 have increased in mice kidneys following cisplatin treatment (n = 4), which was attenuated by myo-inositol treatment.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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GPX4 Antibody - #DF6701