DLC1 Antibody - #DF6693

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Product: DLC1 Antibody
Catalog: DF6693
Description: Rabbit polyclonal antibody to DLC1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 171kDa; 171kD(Calculated).
Uniprot: Q96QB1
RRID: AB_2838655

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
DLC1 Antibody detects endogenous levels of total DLC1.
RRID:
AB_2838655
Cite Format: Affinity Biosciences Cat# DF6693, RRID:AB_2838655.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ARHGAP 7; ARHGAP7; Deleted in liver cancer 1; Deleted in liver cancer 1 protein; DLC 1; Dlc-1; DLC1; DLC1 Rho GTPase activating protein; FLJ21120; HP; HP protein; KIAA1723; p122 RhoGAP; RHG07_HUMAN; Rho GTPase activating protein 7; Rho GTPase-activating protein 7; Rho type GTPase activating protein 7; Rho-type GTPase-activating protein 7; StAR related lipid transfer (START) domain containing 12; StAR related lipid transfer protein 12; StAR-related lipid transfer protein 12; STARD 12; StARD12; START domain containing protein 12; START domain-containing protein 12;

Immunogens

Immunogen:
Uniprot:

Q96QB1(RHG07_HUMAN) >>Visit HPA database.

Gene(ID):
DLC1(10395)
Expression:
Q96QB1 RHG07_HUMAN:

Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.

Description:
This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.
Sequence:
MSVAIRKRSWEEHVTHWMGQPFNSDDRNTACHHGLVADSLQASMEKDATLNVDRKEKCVSLPDCCHGSELRDFPGRPMGHLSKDVDENDSHEGEDQFLSLEASTETLVHVSDEDNNADLCLTDDKQVLNTQGQKTSGQHMIQGAGSLEKALPIIQSNQVSSNSWGIAGETELALVKESGERKVTDSISKSLELCNEISLSEIKDAPKVNAVDTLNVKDIAPEKQLLNSAVIAQQRRKPDPPKDENERSTCNVVQNEFLDTPCTNRGLPLLKTDFGSCLLQPPSCPNGMSAENGLEKSGFSQHQNKSPPKVKAEDGMQCLQLKETLATQEPTDNQVRLRKRKEIREDRDRARLDSMVLLIMKLDQLDQDIENALSTSSSPSGTPTNLRRHVPDLESGSESGADTISVNQTRVNLSSDTESTDLPSSTPVANSGTKPKTTAIQGISEKEKAEIEAKEACDWLRATGFPQYAQLYEDFLFPIDISLVKREHDFLDRDAIEALCRRLNTLNKCAVMKLEISPHRKRSDDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDLVPGSPDDSHPKDGPSPGGTLMDLSERQEVSSVRSLSSTGSLPSHAPPSEDAATPRTNSVISVCSSSNLAGNDDSFGSLPSPKELSSFSFSMKGHEKTAKSKTRSLLKRMESLKLKSSHHSKHKAPSKLGLIISGPILQEGMDEEKLKQLNCVEISALNGNRINVPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSACNKRVGMYLEGFDPFNQSTFNNVVEQNFKNRESYPEDTVFYIPEDHKPGTFPKALTNGSFSPSGNNGSVNWRTGSFHGPGHISLRRENSSDSPKELKRRNSSSSMSSRLSIYDNVPGSILYSSSGDLADLENEDIFPELDDILYHVKGMQRIVNQWSEKFSDEGDSDSALDSVSPCPSSPKQIHLDVDNDRTTPSDLDSTGNSLNEPEEPSEIPERRDSGVGASLTRSNRHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIKVPDYKDRSVFGVPLTVNVQRTGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNEGAIDCVNYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLYFLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHMIAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLGNDDSADYQHFLQDCVDGLFKEVKEKFKGWVSYSTSEQAELSYKKVSEGPPLRLWRSVIEVPAVPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQNSMAPHPARDYVVLRTWRTNLPKGACALLLTSVDHDRAPVVGVRVNVLLSRYLIEPCGPGKSKLTYMCRVDLRGHMPEWYTKSFGHLCAAEVVKIRDSFSNQNTETKDTKSR

PTMs - Q96QB1 As Substrate

Site PTM Type Enzyme
S354 Phosphorylation
S431 Phosphorylation
K513 Sumoylation
S523 Phosphorylation
S526 Phosphorylation
S535 Phosphorylation
S544 Phosphorylation
S548 Phosphorylation
S557 Phosphorylation
S566 Phosphorylation
S577 Phosphorylation
S642 Phosphorylation
S652 Phosphorylation
S673 Phosphorylation
K707 Ubiquitination
S733 Phosphorylation
S735 Phosphorylation
S737 Phosphorylation
T738 Phosphorylation
T740 Phosphorylation
S743 Phosphorylation
S745 Phosphorylation
S747 Phosphorylation
S750 Phosphorylation
T755 Phosphorylation
S757 Phosphorylation
S766 Phosphorylation P31749 (AKT1)
T840 Phosphorylation
S842 Phosphorylation
S850 Phosphorylation
S859 Phosphorylation
S928 Phosphorylation
S946 Phosphorylation
S986 Phosphorylation
S991 Phosphorylation
T993 Phosphorylation
S995 Phosphorylation
S1004 Phosphorylation
K1051 Ubiquitination
S1055 Phosphorylation
S1209 Phosphorylation
T1238 Phosphorylation
S1244 Phosphorylation Q15139 (PRKD1)
S1355 Phosphorylation
T1435 Phosphorylation

Research Backgrounds

Function:

Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality.

Subcellular Location:

Cytoplasm. Cell junction>Focal adhesion. Membrane>Peripheral membrane protein.
Note: Colocalizes with EF1A1 at actin-rich regions in the cell periphery.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.

Subunit Structure:

Interacts with EF1A1, facilitates EF1A1 distribution to the membrane periphery and ruffles upon growth factor stimulation and suppresses cell migration.

Family&Domains:

The SAM domain mediates interaction with EF1A1, and functions as an autoinhibitory regulator of RhoGAP Activity.

The polybasic cluster is required for activation and mediates binding to phosphatidylinositol-4,5-bisphosphate (PI(4,5)P(2)) containing membranes.

Restrictive clause

 

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