PPP2R4 Antibody | Affinity Biosciences

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Western blot analysis of extracts from HepG2, using PPP2R4 Antibody.

Western blot analysis of extracts from various samples, using PPP2R4 Antibody.

DF6688 at 1/100 staining Human prostate cancer by IHC-P.

DF6688 at 1/100 staining Rat brain tissue by IHC-P.

Product:	PPP2R4 Antibody
Catalog:	DF6688
Description:	Rabbit polyclonal antibody to PPP2R4
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	40kDa; 41kD(Calculated).
Uniprot:	Q15257
RRID:	AB_2838650

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%), Xenopus(89%)

Clonality:

Polyclonal

Specificity:

PPP2R4 Antibody detects endogenous levels of total PPP2R4.

RRID:

AB_2838650
Cite Format: Affinity Biosciences Cat# DF6688, RRID:AB_2838650.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

subunit B''; KIAA0044; MGC2184; Phosphotyrosyl phosphatase activator; PP2A; PP2A phosphatase activator; PP2A subunit B'; PP2A subunit B' isoform PR53; PP2A subunit B' PR53 isoform; PPP2R4; PR53; PR53 isoform; Protein phosphatase 2, regulatory subunit B-prime; Protein phosphatase 2A activator regulatory subunit 4; Protein phosphatase 2A regulatory subunit B' (PR 53); PTPA; PTPA_HUMAN; Serine/threonine protein phosphatase 2A regulatory subunit B'; Serine/threonine-protein phosphatase 2A activator; Serine/threonine-protein phosphatase 2A regulatory subunit 4; Serine/threonine-protein phosphatase 2A regulatory subunit B'';

Immunogens

Immunogen:

Uniprot:

Q15257(PTPA_HUMAN) >>Visit HPA database.

Gene(ID):

PTPA(5524)

Expression:

Q15257 PTPA_HUMAN:

Widely expressed.

Description:

Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.

Sequence:

Q15257 PTPA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAEGERQPPPDSSEEAPPATQNFIIPKKEIHTVPDMGKWKRSQAYADYIGFILTLNEGVKGKKLTFEYRVSEMWNEVHEEKEQAAKQSVSCDECIPLPRAGHCAPSEAIEKLVALLNTLDRWIDETPPVDQPSRFGNKAYRTWYAKLDEEAENLVATVVPTHLAAAVPEVAVYLKESVGNSTRIDYGTGHEAAFAAFLCCLCKIGVLRVDDQIAIVFKVFNRYLEVMRKLQKTYRMEPAGSQGVWGLDDFQFLPFIWGSSQLIDHPYLEPRHFVDEKAVNENHKDYMFLECILFITEMKTGPFAEHSNQLWNISAVPSWSKVNQGLIRMYKAECLEKFPVIQHFKFGSLLPIHPVTSG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15257 As Substrate

Q15257

Site	PTM Type	Source
A2	Acetylation	Uniprot
K27	Ubiquitination	Uniprot
K28	Ubiquitination	Uniprot
K38	Ubiquitination	Uniprot
K62	Acetylation	Uniprot
K63	Ubiquitination	Uniprot
S90	Phosphorylation	Uniprot
T118	Phosphorylation	Uniprot
R121	Methylation	Uniprot
K138	Ubiquitination	Uniprot
R141	Methylation	Uniprot
Y223	Phosphorylation	Uniprot
K277	Ubiquitination	Uniprot
K321	Acetylation	Uniprot
K321	Ubiquitination	Uniprot
K331	Acetylation	Uniprot
K331	Ubiquitination	Uniprot
K337	Acetylation	Uniprot
K337	Ubiquitination	Uniprot
K345	Acetylation	Uniprot
K345	Ubiquitination	Uniprot
S357	Phosphorylation	Uniprot

Research Backgrounds

Q15257_PTPA_HUMAN

Function:

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Acts as a regulatory subunit for serine/threonine-protein phosphatase 2A (PP2A) modulating its activity or substrate specificity, probably by inducing a conformational change in the catalytic subunit, a proposed direct target of the PPIase. Can reactivate inactive phosphatase PP2A-phosphatase methylesterase complexes (PP2A(i)) in presence of ATP and Mg(2+) (By similarity). Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer PP2A(D) in presence of ATP and Mg(2+) (in vitro). The phosphotyrosyl phosphatase activity is dependent of an ATPase activity of the PP2A(D):PPP2R4 complex. Is involved in apoptosis; the function appears to be independent from PP2A.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Associates with PP2A heterodimeric core enzyme PP2A(D), composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A). Interacts with PPP2CB (By similarity).

Family&Domains:

Belongs to the PTPA-type PPIase family.

Research Fields

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

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PPP2R4 Antibody - #DF6688