DNA Ligase III Antibody - #DF6677

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Product: DNA Ligase III Antibody
Catalog: DF6677
Description: Rabbit polyclonal antibody to DNA Ligase III
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 113kDa; 113kD(Calculated).
Uniprot: P49916
RRID: AB_2838639

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DNA Ligase III Antibody detects endogenous levels of total DNA Ligase III.
RRID:
AB_2838639
Cite Format: Affinity Biosciences Cat# DF6677, RRID:AB_2838639.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

D11Wsu78e; DNA ligase; DNA ligase 3; DNA ligase III; DNLI3_HUMAN; LIG 3; LIG2; LIG3; Ligase II, DNA, ATP dependent; Ligase III DNA ATP dependent; MGC78176; OTTMUSP00000000875; Polydeoxyribonucleotide synthase [ATP] 3; Polydeoxyribonucleotide synthase [ATP]; Polydeoxyribonucleotide synthase; RP23-316C11.6;

Immunogens

Immunogen:
Uniprot:

P49916(DNLI3_HUMAN) >>Visit HPA database.

Gene(ID):
LIG3(3980)
Expression:
P49916 DNLI3_HUMAN:

Testis, thymus, prostate and heart.

Description:
This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Sequence:
MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
78
Chicken
78
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49916 As Substrate

Site PTM Type Enzyme
K111 Acetylation
K114 Sumoylation
K114 Ubiquitination
K121 Ubiquitination
K146 Ubiquitination
K156 Ubiquitination
T161 Phosphorylation
T191 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
T204 Phosphorylation
T205 Phosphorylation
T209 Phosphorylation
S210 Phosphorylation P24941 (CDK2) , P06493 (CDK1)
K213 Methylation
K213 Ubiquitination
S216 Phosphorylation
S227 Phosphorylation
K232 Acetylation
K232 Ubiquitination
S236 Phosphorylation
S241 Phosphorylation
S242 Phosphorylation
T244 Phosphorylation
K316 Acetylation
K324 Ubiquitination
K369 Ubiquitination
K389 Ubiquitination
K427 Ubiquitination
K442 Ubiquitination
K463 Ubiquitination
S472 Phosphorylation
S476 Phosphorylation
T479 Phosphorylation
K498 Ubiquitination
K531 Ubiquitination
K570 Ubiquitination
K581 Ubiquitination
K615 Acetylation
K615 Ubiquitination
T637 Phosphorylation
K638 Ubiquitination
T647 Phosphorylation
K660 Ubiquitination
K663 Ubiquitination
T665 Phosphorylation
Y666 Phosphorylation
S720 Phosphorylation
K728 Ubiquitination
K749 Ubiquitination
K756 Ubiquitination
K762 Ubiquitination
K765 Ubiquitination
Y767 Phosphorylation
Y768 Phosphorylation
T796 Phosphorylation
K817 Methylation
K817 Sumoylation
K817 Ubiquitination
K826 Ubiquitination
K833 Ubiquitination
S853 Phosphorylation
S871 Phosphorylation
S874 Phosphorylation
S876 Phosphorylation
T877 Phosphorylation
K883 Acetylation
S889 Phosphorylation
S900 Phosphorylation
T910 Phosphorylation
S912 Phosphorylation
S913 Phosphorylation P06493 (CDK1) , P24941 (CDK2)

Research Backgrounds

Function:

Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair.

Subcellular Location:

Mitochondrion.
Note: Contains an N-terminal mitochondrial transit peptide.

Mitochondrion.
Note: Contains an N-terminal mitochondrial transit peptide.

Nucleus.
Note: Lacks the N-terminal mitochondrial transit peptide.

Nucleus.
Note: Lacks the N-terminal mitochondrial transit peptide.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Testis, thymus, prostate and heart.

Subunit Structure:

Isoform 3 interacts (via BRCT domain) with the nuclear DNA-repair protein XRCC1.

Family&Domains:

The PARP-type zinc finger is required for DNA ligase activity.

Belongs to the ATP-dependent DNA ligase family.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

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