EPXH2 Antibody - #DF6676

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Product: EPXH2 Antibody
Catalog: DF6676
Description: Rabbit polyclonal antibody to EPXH2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 62kDa; 63kD(Calculated).
Uniprot: P34913
RRID: AB_2838638

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%)
Clonality:
Polyclonal
Specificity:
EPXH2 Antibody detects endogenous levels of total EPXH2.
RRID:
AB_2838638
Cite Format: Affinity Biosciences Cat# DF6676, RRID:AB_2838638.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Bifunctional epoxide hydrolase 2; CEH; Cytosolic epoxide hydrolase; EPHX2; Epoxide hydratase; Epoxide hydrolase 2; Epoxide hydrolase 2 cytoplasmic; epoxide hydrolase 2, cytosolic; Epoxide hydrolase soluble; HYES_HUMAN; SEH; Soluble epoxide hydrolase;

Immunogens

Immunogen:
Uniprot:

P34913(HYES_HUMAN) >>Visit HPA database.

Gene(ID):
EPHX2(2053)
Description:
This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes,binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have beenassociated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.
Sequence:
MTLRAAVFDLDGVLALPAVFGVLGRTEEALALPRGLLNDAFQKGGPEGATTRLMKGEITLSQWIPLMEENCRKCSETAKVCLPKNFSIKEIFDKAISARKINRPMLQAALMLRKKGFTTAILTNTWLDDRAERDGLAQLMCELKMHFDFLIESCQVGMVKPEPQIYKFLLDTLKASPSEVVFLDDIGANLKPARDLGMVTILVQDTDTALKELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHGFPESWYSWRYQIPALAQAGYRVLAMDMKGYGESSAPPEIEEYCMEVLCKEMVTFLDKLGLSQAVFIGHDWGGMLVWYMALFYPERVRAVASLNTPFIPANPNMSPLESIKANPVFDYQLYFQEPGVAEAELEQNLSRTFKSLFRASDESVLSMHKVCEAGGLFVNSPEEPSLSRMVTEEEIQFYVQQFKKSGFRGPLNWYRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHMEDWIPHLKRGHIEDCGHWTQMDKPTEVNQILIKWLDSDARNPPVVSKM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Bovine
91
Sheep
91
Rabbit
91
Zebrafish
73
Xenopus
70
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P34913 As Substrate

Site PTM Type Enzyme
K43 Acetylation
K43 Ubiquitination
K55 Ubiquitination
K79 Ubiquitination
K84 Ubiquitination
K89 Ubiquitination
K94 Acetylation
K94 Ubiquitination
K114 Acetylation
K115 Acetylation
K167 Acetylation
K174 Acetylation
K174 Ubiquitination
K191 Ubiquitination
K245 Ubiquitination
Y296 Phosphorylation
S299 Phosphorylation
Y308 Phosphorylation
S370 Phosphorylation
K406 Ubiquitination
S412 Phosphorylation
K421 Ubiquitination
K455 Acetylation
K474 Ubiquitination
K478 Ubiquitination
K483 Ubiquitination
K515 Ubiquitination
K530 Ubiquitination
K540 Ubiquitination
K554 Ubiquitination

Research Backgrounds

Function:

Bifunctional enzyme. The C-terminal domain has epoxide hydrolase activity and acts on epoxides (alkene oxides, oxiranes) and arene oxides. Plays a role in xenobiotic metabolism by degrading potentially toxic epoxides (By similarity). Also determines steady-state levels of physiological mediators. The N-terminal domain has lipid phosphatase activity, with the highest activity towards threo-9,10-phosphonooxy-hydroxy-octadecanoic acid, followed by erythro-9,10-phosphonooxy-hydroxy-octadecanoic acid, 12-phosphonooxy-octadec-9Z-enoic acid and 12-phosphonooxy-octadec-9E-enoic acid.

PTMs:

The N-terminus is blocked.

The covalent modification of cysteine by 15-deoxy-Delta12,14-prostaglandin-J2 is autocatalytic and reversible. It may occur as an alternative to other cysteine modifications, such as S-nitrosylation and S-palmitoylation (Probable).

Subcellular Location:

Cytoplasm. Peroxisome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

The N-terminal domain has phosphatase activity. The C-terminal domain has epoxide hydrolase activity.

Belongs to the AB hydrolase superfamily. Epoxide hydrolase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Ginsenoside compound K alleviates sodium valproate-induced hepatotoxicity in rats via antioxidant effect, regulation of peroxisome pathway and iron homeostasis. TOXICOLOGY AND APPLIED PHARMACOLOGY, 2020 (PubMed: 31734319) [IF=3.8]

Application: WB    Species: rat    Sample: liver

Fig. 7| Effect of SVP alone and combined with G-CK or L-Car on hepatic soluble epoxide hydrolase protein level. A: Western blot analysis
2). Evaluating the protective effects of individual or combined ginsenoside compound K and the downregulation of soluble epoxide hydrolase expression against sodium valproate-induced liver cell damage. Toxicology and Applied Pharmacology, 2021 (PubMed: 33915122) [IF=3.8]

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