SLC25A4 Antibody - #DF6674

(3)  
Product: SLC25A4 Antibody
Catalog: DF6674
Description: Rabbit polyclonal antibody to SLC25A4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 33kDa; 33kD(Calculated).
Uniprot: P12235
RRID: AB_2838636

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
SLC25A4 Antibody detects endogenous levels of total SLC25A4.
RRID:
AB_2838636
Cite Format: Affinity Biosciences Cat# DF6674, RRID:AB_2838636.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AAC1; Adenine nucleotide translocator 1 (skeletal muscle); Adenine nucleotide translocator 1; ADP; ADP ATP carrier protein 1; ADP ATP carrier protein heart/skeletal muscle isoform T1; ADP/ATP translocase 1; ADT1_HUMAN; ANT 1; ANT; ANT1; ATP carrier protein 1; ATP carrier protein; heart/skeletal muscle isoform T1; PEO2; PEO3; SLC25A4; Solute carrier family 25 member 4; T1;

Immunogens

Immunogen:
Uniprot:

P12235(ADT1_HUMAN) >>Visit HPA database.

Gene(ID):
SLC25A4(291)
Description:
This gene is a member of the mitochondrial carrier subfamily of solute carrier protein genes. The product of this gene functions as a gated pore that translocates ADP from the cytoplasm into the mitochondrial matrix and ATP from the mitochondrial matrix into the cytoplasm. The protein forms a homodimer embedded in the inner mitochondria membrane. Mutations in this gene have been shown to result in autosomal dominant progressive external opthalmoplegia and familial hypertrophic cardiomyopathy.
Sequence:
MGDHAWSFLKDFLAGGVAAAVSKTAVAPIERVKLLLQVQHASKQISAEKQYKGIIDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKQLFLGGVDRHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGAAQREFHGLGDCIIKIFKSDGLRGLYQGFNVSVQGIIIYRAAYFGVYDTAKGMLPDPKNVHIFVSWMIAQSVTAVAGLVSYPFDTVRRRMMMQSGRKGADIMYTGTVDCWRKIAKDEGAKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12235 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
K10 Acetylation
S22 Phosphorylation
K23 Acetylation
T24 Phosphorylation
K33 Acetylation
K33 Ubiquitination
S42 Phosphorylation
K43 Acetylation
K43 Ubiquitination
K52 Acetylation
K52 Methylation
K52 Ubiquitination
K63 Ubiquitination
Y81 Phosphorylation
T84 Phosphorylation
K92 Acetylation
K92 Ubiquitination
K94 Acetylation
K96 Acetylation
K96 Ubiquitination
K107 Ubiquitination
Y112 Phosphorylation
S119 Phosphorylation
T139 Phosphorylation
R140 Methylation
K147 Methylation
K147 Ubiquitination
K166 Acetylation
S167 Phosphorylation
Y174 Phosphorylation
Y187 Phosphorylation
Y191 Phosphorylation
Y195 Phosphorylation P06239 (LCK) , P12931 (SRC)
T197 Phosphorylation
K199 Ubiquitination
S242 Phosphorylation
K245 Ubiquitination
K272 Acetylation
K272 Methylation
K272 Ubiquitination
S276 Phosphorylation
K295 Acetylation
K295 Ubiquitination

Research Backgrounds

Function:

Involved in mitochondrial ADP/ATP transport. Catalyzes the exchange of cytoplasmic ADP with mitochondrial ATP across the mitochondrial inner membrane.

Subcellular Location:

Mitochondrion inner membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Found in a complex with ARL2, ARL2BP and SLC25A4. Interacts with ARL2BP (By similarity). Homodimer.

(Microbial infection) Interacts with HIV-1 Vpr.

Family&Domains:

The transmembrane helices are not perpendicular to the plane of the membrane, but cross the membrane at an angle. Odd-numbered transmembrane helices exhibit a sharp kink, due to the presence of a conserved proline residue.

Belongs to the mitochondrial carrier (TC 2.A.29) family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Parkinson's disease.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

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