ABCG8 Antibody - #DF6673

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Product: ABCG8 Antibody
Catalog: DF6673
Description: Rabbit polyclonal antibody to ABCG8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 76kDa; 76kD(Calculated).
Uniprot: Q9H221
RRID: AB_2838635

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(91%), Horse(100%), Sheep(91%), Rabbit(91%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ABCG8 Antibody detects endogenous levels of total ABCG8.
RRID:
AB_2838635
Cite Format: Affinity Biosciences Cat# DF6673, RRID:AB_2838635.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ABCG8; ABCG8_HUMAN; ATP binding cassette sub family G (WHITE) member 8 (sterolin 2); ATP binding cassette sub family G member 8; ATP binding cassette, sub family G (WHITE), member 8; ATP-binding cassette sub-family G member 8; GBD4; MGC142217; Sterolin 2; Sterolin-2; STSL;

Immunogens

Immunogen:
Uniprot:

Q9H221(ABCG8_HUMAN) >>Visit HPA database.

Gene(ID):
ABCG8(64241)
Expression:
Q9H221 ABCG8_HUMAN:

Predominantly expressed in the liver (PubMed:11099417, PubMed:11452359). Low expression levels in the small intestine and colon (PubMed:11099417). Very low levels in other tissues, including brain, heart and spleen (PubMed:11452359).

Description:
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. The protein encoded by this gene functions to exclude non-cholesterol sterol entry at the intestinal level, promote excretion of cholesterol and sterols into bile, and to facilitate transport of sterols back into the intestinal lumen. It is expressed in a tissue-specific manner in the liver, intestine, and gallbladder. This gene is tandemly arrayed on chromosome 2, in a head-to-head orientation with family member ABCG5. Mutations in this gene may contribute to sterol accumulation and atherosclerosis, and have been observed in patients with sitosterolemia. [provided by RefSeq, Jul 2008]
Sequence:
MAGKAAEERGLPKGATPQDTSGLQDRLFSSESDNSLYFTYSGQPNTLEVRDLNYQVDLASQVPWFEQLAQFKMPWTSPSCQNSCELGIQNLSFKVRSGQMLAIIGSSGCGRASLLDVITGRGHGGKIKSGQIWINGQPSSPQLVRKCVAHVRQHNQLLPNLTVRETLAFIAQMRLPRTFSQAQRDKRVEDVIAELRLRQCADTRVGNMYVRGLSGGERRRVSIGVQLLWNPGILILDEPTSGLDSFTAHNLVKTLSRLAKGNRLVLISLHQPRSDIFRLFDLVLLMTSGTPIYLGAAQHMVQYFTAIGYPCPRYSNPADFYVDLTSIDRRSREQELATREKAQSLAALFLEKVRDLDDFLWKAETKDLDEDTCVESSVTPLDTNCLPSPTKMPGAVQQFTTLIRRQISNDFRDLPTLLIHGAEACLMSMTIGFLYFGHGSIQLSFMDTAALLFMIGALIPFNVILDVISKCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKPSQDW

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Chicken
100
Bovine
91
Sheep
91
Rabbit
91
Zebrafish
73
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H221 As Substrate

Site PTM Type Enzyme
T166 Phosphorylation
Y209 Phosphorylation
S214 Phosphorylation
Y314 Phosphorylation
S315 Phosphorylation
T325 Phosphorylation
S331 Phosphorylation

Research Backgrounds

Function:

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG5 has ATPase activity.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Apical cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in the liver. Low expression levels in the small intestine and colon. Very low levels in other tissues, including brain, heart and spleen.

Subunit Structure:

Heterodimer with ABCG8.

Family&Domains:

A functional Walker motif (consensus sequence G-X-X-G-X-G-K-[ST]-T) is expected to bind ATP. The essential Lys in this region is not conserved in ABCG8 (G-S-S-G-C-R-A-S) and is not required for transport activity mediated by the heterodimer with ABCG5.

Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.

Research Fields

· Environmental Information Processing > Membrane transport > ABC transporters.

· Organismal Systems > Digestive system > Fat digestion and absorption.

· Organismal Systems > Digestive system > Cholesterol metabolism.

References

1). Hif-1α expression targets the TMA/Fmo3/TMAO axis to participate in gallbladder cholesterol stone formation in individuals living in plateau regions. Biochimica et biophysica acta. Molecular basis of disease, 2024 (PubMed: 38657913) [IF=6.2]
2). Astragalus polysaccharides ameliorate diet-induced gallstone formation by modulating synthesis of bile acids and the gut microbiota. Frontiers in Pharmacology, 2021 (PubMed: 34276384) [IF=5.6]

Application: WB    Species: Mice    Sample: liver

FIGURE 5 Expression of the genes involved in cholesterol transport and bile-acid synthesis in the liver. (A) mRNA expression of Cyp7a1, Cyp8b1, Cyp7b1, and Cyp27a1 (n = 8). (B) Protein expression (left panel) and quantification (middle and right panel) of Cyp7a1 and Cyp7b1 (n = 6). (C) mRNA expression of Fgfr4 and β-klotho (n = 8). (D) mRNA expression of Abcg5 and Abcg8 (n = 8). (E) Protein expression (left panel) and quantification (middle and right panel) of Abcg5 and Abcg8 (n = 6). Data are the mean ± SD. *p < 0.05, **p < 0.01. Chow, chow diet; LD, lithogenic diet; AH, high-dose APS.
3). Ganoderma lucidum polysaccharide ameliorates cholesterol gallstone formation by modulating cholesterol and bile acid metabolism in an FXR-dependent manner. Chinese medicine, 2024 (PubMed: 38268006) [IF=4.9]
4). Gypenosides Prevent and Dissolve Cholesterol Gallstones by Modulating the Homeostasis of Cholesterol and Bile Acids. Frontiers in Medicine, 2022 (PubMed: 35445045) [IF=3.9]

Application: WB    Species: Mice    Sample:

Figure 7 Hepatic expression of genes involved in cholesterol transport and synthesis. (A) Levels of expression of Abcg5 and Abcg8 mRNAs measured by qRT-PCR (left panel, n = 8) and RNA sequencing (RNA-seq) analyses (right panel, data shown as means). (B) Protein expression and quantification of Abcg5 and Abcg8 (n = 6). (C) Levels of expression of Lxrα, Hmgcr and Srebp2 mRNAs measured by qRT-PCR (left panel, n = 8) and RNA-seq analyses (right panel, data shown as means). Data are reported as means ± SEM. **P < 0.01.
5). Identification and Analysis of Three Hub Prognostic Genes Related to Osteosarcoma Metastasis. Journal of Oncology, 2021 (PubMed: 33564309)

Application: IHC    Species: Rat    Sample: tumor tissues

Figure 14 Immunohistochemical staining results.

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