LY96 Antibody | Affinity Biosciences

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Western blot analysis of Rat spleen lysates, using LY96 Antibody.

Product:	LY96 Antibody
Catalog:	DF6669
Description:	Rabbit polyclonal antibody to LY96
Application:	WB IHC
Reactivity:	Human, Rat
Prediction:	Horse, Dog
Mol.Wt.:	18kDa; 19kD(Calculated).
Uniprot:	Q9Y6Y9
RRID:	AB_2838631

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Horse(100%), Dog(89%)

Clonality:

Polyclonal

Specificity:

LY96 Antibody detects endogenous levels of total LY96.

RRID:

AB_2838631
Cite Format: Affinity Biosciences Cat# DF6669, RRID:AB_2838631.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ESOP 1; ESOP-1; ESOP1; LY 96; Ly-96; LY96; LY96_HUMAN; Lymphocyte antigen 96; md 2; MD 2 protein; MD2 protein; Myeloid differentiation protein 2; Protein MD 2; Protein MD-2; Protein MD2;

Immunogens

Immunogen:

Uniprot:

Q9Y6Y9(LY96_HUMAN) >>Visit HPA database.

Gene(ID):

LY96(23643)

Description:

This gene encodes a protein which associates with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. Studies of the mouse ortholog suggest that this gene may be involved in endotoxin neutralization. Alternative splicing results in multiple transcript variants encoding different isoforms.

Sequence:

Q9Y6Y9 LY96_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLPFLFFSTLFSSIFTEAQKQYWVCNSSDASISYTYCDKMQYPISINVNPCIELKRSKGLLHIFYIPRRDLKQLYFNLYITVNTMNLPKRKEVICRGSDDDYSFCRALKGETVNTTISFSFKGIKFSKGKYKCVVEAISGSPEEMLFCLEFVILHQPNSN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

Sheep

Rabbit

Chicken

Pig

Bovine

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6Y9 As Substrate

Q9Y6Y9

Site	PTM Type	Enzyme	Source
Y22	Phosphorylation	P07948 (LYN)	Uniprot
N26	N-Glycosylation		Uniprot
N114	N-Glycosylation		Uniprot
Y131	Phosphorylation	P07948 (LYN)	Uniprot

Research Backgrounds

Q9Y6Y9_LY96_HUMAN

Function:

Binds bacterial lipopolysaccharide (LPS). Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS.

PTMs:

N-glycosylated; high-mannose.

Subcellular Location:

Secreted>Extracellular space. Secreted.
Note: Retained in the extracellular space at the cell surface by interaction with TLR4 (PubMed:10359581).

Subunit Structure:

Heterogeneous homopolymer formed from homodimers; disulfide-linked. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binds to the extracellular domains of TLR2 and TLR4. Ligand binding induces interaction with TLR4 and oligomerization of the complex.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)

References

1). Patchouli alcohol ameliorates acute liver injury via inhibiting oxidative stress and gut-origin LPS leakage in rats. International Immunopharmacology, 2021 (PubMed: 34182243) [IF=5.6]

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For Research Use Only.
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LY96 Antibody - #DF6669