GLB1 Antibody | Affinity Biosciences

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Cites

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Western blot analysis of A549 cell lysates, using GLB1 Antibody.

FIGURE 4 Effects of cyclophosphamide (CTX) on Ki67, β-gal, p21, p53, γH2AX, and pATM protein expression.

Product:	GLB1 Antibody
Catalog:	DF6667
Description:	Rabbit polyclonal antibody to GLB1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	76kDa; 76kD(Calculated).
Uniprot:	P16278
RRID:	AB_2838629

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

GLB1 Antibody detects endogenous levels of total GLB1.

RRID:

AB_2838629
Cite Format: Affinity Biosciences Cat# DF6667, RRID:AB_2838629.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Acid beta galactosidase; Acid beta-galactosidase; Beta galactosidase 1; Beta galactosidase; Beta-galactosidase; BGAL_HUMAN; EBP; EBP, included; Elastin receptor 1 (67kD); Elastin receptor 1 67kDa; Elastin receptor 1; Elastin receptor 1, included; Elastin-binding protein, included; ELNR1; Galactosidase beta 1; GLB 1; GLB1; Lactase; MPS4B; S-GAL, included;

Immunogens

Immunogen:

Uniprot:

P16278(BGAL_HUMAN) >>Visit HPA database.

Gene(ID):

GLB1(2720)

Expression:

P16278 BGAL_HUMAN:

Detected in placenta (at protein level) (PubMed:8383699). Detected in fibroblasts and testis (PubMed:2511208).

Description:

This gene encodes beta-galactosidase-1, a lysosomal enzyme that hydrolyzes the terminal beta-galactose from ganglioside substrates and other glycoconjugates. Defects in this gene are the cause of GM1-gangliosidosis and Morquio B syndrome. Multiple transcript variants encoding different isoforms have been found for this gene.

Sequence:

P16278 BGAL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPGFLVRILPLLLVLLLLGPTRGLRNATQRMFEIDYSRDSFLKDGQPFRYISGSIHYSRVPRFYWKDRLLKMKMAGLNAIQTYVPWNFHEPWPGQYQFSEDHDVEYFLRLAHELGLLVILRPGPYICAEWEMGGLPAWLLEKESILLRSSDPDYLAAVDKWLGVLLPKMKPLLYQNGGPVITVQVENEYGSYFACDFDYLRFLQKRFRHHLGDDVVLFTTDGAHKTFLKCGALQGLYTTVDFGTGSNITDAFLSQRKCEPKGPLINSEFYTGWLDHWGQPHSTIKTEAVASSLYDILARGASVNLYMFIGGTNFAYWNGANSPYAAQPTSYDYDAPLSEAGDLTEKYFALRNIIQKFEKVPEGPIPPSTPKFAYGKVTLEKLKTVGAALDILCPSGPIKSLYPLTFIQVKQHYGFVLYRTTLPQDCSNPAPLSSPLNGVHDRAYVAVDGIPQGVLERNNVITLNITGKAGATLDLLVENMGRVNYGAYINDFKGLVSNLTLSSNILTDWTIFPLDTEDAVRSHLGGWGHRDSGHHDEAWAHNSSNYTLPAFYMGNFSIPSGIPDLPQDTFIQFPGWTKGQVWINGFNLGRYWPARGPQLTLFVPQHILMTSAPNTITVLELEWAPCSSDDPELCAVTFVDRPVIGSSVTYDHPSKPVEKRLMPPPPQKNKDSWLDHV

PTMs - P16278 As Substrate

P16278

Site	PTM Type	Source
T21	Phosphorylation	Uniprot
S37	Phosphorylation	Uniprot
S40	Phosphorylation	Uniprot
K43	Methylation	Uniprot
K43	Ubiquitination	Uniprot
Y57	Phosphorylation	Uniprot
S58	Phosphorylation	Uniprot
Y154	Phosphorylation	Uniprot
N247	N-Glycosylation	Uniprot
T286	Phosphorylation	Uniprot
Y294	Phosphorylation	Uniprot
S368	Phosphorylation	Uniprot
T369	Phosphorylation	Uniprot
K383	Ubiquitination	Uniprot
N464	N-Glycosylation	Uniprot
Y485	Phosphorylation	Uniprot
Y488	Phosphorylation	Uniprot
K493	Acetylation	Uniprot
N498	N-Glycosylation	Uniprot
N555	N-Glycosylation	Uniprot
K670	Ubiquitination	Uniprot

Research Backgrounds

P16278_BGAL_HUMAN

Function:

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.

Has no beta-galactosidase catalytic activity, but plays functional roles in the formation of extracellular elastic fibers (elastogenesis) and in the development of connective tissue. Seems to be identical to the elastin-binding protein (EBP), a major component of the non-integrin cell surface receptor expressed on fibroblasts, smooth muscle cells, chondroblasts, leukocytes, and certain cancer cell types. In elastin producing cells, associates with tropoelastin intracellularly and functions as a recycling molecular chaperone which facilitates the secretions of tropoelastin and its assembly into elastic fibers.

Subcellular Location:

Lysosome.

Cytoplasm>Perinuclear region.
Note: Localized to the perinuclear area of the cytoplasm but not to lysosomes.

Tissue Specificity:

Detected in placenta (at protein level). Detected in fibroblasts and testis.

Subunit Structure:

Homodimer. May form higher multimers (Probable).

Family&Domains:

Belongs to the glycosyl hydrolase 35 family.

Research Fields

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Metabolism > Carbohydrate metabolism > Galactose metabolism.

· Metabolism > Glycan biosynthesis and metabolism > Other glycan degradation.

· Metabolism > Glycan biosynthesis and metabolism > Glycosaminoglycan degradation.

· Metabolism > Lipid metabolism > Sphingolipid metabolism.

· Metabolism > Glycan biosynthesis and metabolism > Glycosphingolipid biosynthesis - ganglio series.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Role of DNA damage response in cyclophosphamide-induced premature ovarian failure in mice. The journal of obstetrics and gynaecology research, 2024 (PubMed: 38936810) [IF=1.6]

Application: IHC Species: Mouse Sample:

FIGURE 4 Effects of cyclophosphamide (CTX) on Ki67, β-gal, p21, p53, γH2AX, and pATM protein expression. Immunohistochemistry was used to observe the cellular locations of these proteins.

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GLB1 Antibody - #DF6667