LIG1 Antibody - #DF6663

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Product: LIG1 Antibody
Catalog: DF6663
Description: Rabbit polyclonal antibody to LIG1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 102kDa; 102kD(Calculated).
Uniprot: P18858
RRID: AB_2838625

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Zebrafish(89%), Bovine(89%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
LIG1 Antibody detects endogenous levels of total LIG1.
RRID:
AB_2838625
Cite Format: Affinity Biosciences Cat# DF6663, RRID:AB_2838625.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA ligase 1; DNA ligase I; DNLI1_HUMAN; LIG 1; lig1; Ligase I DNA ATP dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1;

Immunogens

Immunogen:
Uniprot:

P18858(DNLI1_HUMAN) >>Visit HPA database.

Gene(ID):
LIG1(3978)
Description:
LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq, Jul 2008]
Sequence:
MQRSIMSFFHPKKEGKAKKPEKEASNSSRETEPPPKAALKEWNGVVSESDSPVKRPGRKAARVLGSEGEEEDEALSPAKGQKPALDCSQVSPPRPATSPENNASLSDTSPMDSSPSGIPKRRTARKQLPKRTIQEVLEEQSEDEDREAKRKKEEEEEETPKESLTEAEVATEKEGEDGDQPTTPPKPLKTSKAETPTESVSEPEVATKQELQEEEEQTKPPRRAPKTLSSFFTPRKPAVKKEVKEEEPGAPGKEGAAEGPLDPSGYNPAKNNYHPVEDACWKPGQKVPYLAVARTFEKIEEVSARLRMVETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLELGVGDGVLLKAVAQATGRQLESVRAEAAEKGDVGLVAENSRSTQRLMLPPPPLTASGVFSKFRDIARLTGSASTAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAALSQAVSLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLDRIIPVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEEAAFTCEYKYDGQRAQIHALEGGEVKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
89
Bovine
89
Sheep
89
Zebrafish
89
Xenopus
78
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P18858 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
K40 Ubiquitination
S47 Phosphorylation
S49 Phosphorylation
S51 Phosphorylation P24941 (CDK2)
S66 Phosphorylation P68400 (CSNK2A1)
S76 Phosphorylation P06493 (CDK1) , O14757 (CHEK1) , P24941 (CDK2)
K79 Ubiquitination
S88 Phosphorylation
S91 Phosphorylation P24941 (CDK2)
T97 Phosphorylation
S98 Phosphorylation
S109 Phosphorylation
S114 Phosphorylation
S141 Phosphorylation
R146 Methylation
S163 Phosphorylation
T165 Phosphorylation
T171 Phosphorylation
T182 Phosphorylation
T183 Phosphorylation
T190 Phosphorylation
K192 Ubiquitination
T195 Phosphorylation P24941 (CDK2)
T197 Phosphorylation P24941 (CDK2)
S199 Phosphorylation
S201 Phosphorylation
T207 Phosphorylation
K219 Acetylation
K219 Ubiquitination
K226 Ubiquitination
S229 Phosphorylation
S230 Phosphorylation
T233 Phosphorylation P24941 (CDK2)
K244 Sumoylation
K244 Ubiquitination
K270 Ubiquitination
K282 Ubiquitination
K286 Ubiquitination
K298 Ubiquitination
T311 Phosphorylation
S313 Phosphorylation
K376 Ubiquitination
K407 Ubiquitination
K422 Ubiquitination
K428 Ubiquitination
S437 Phosphorylation
S445 Phosphorylation
S447 Phosphorylation
K493 Ubiquitination
T506 Phosphorylation
K533 Ubiquitination
S535 Phosphorylation
K541 Ubiquitination
T548 Phosphorylation
S552 Phosphorylation
K556 Ubiquitination
K568 Ubiquitination
K585 Ubiquitination
K597 Ubiquitination
K630 Sumoylation
K630 Ubiquitination
T639 Phosphorylation
S714 Phosphorylation
K737 Ubiquitination
K744 Ubiquitination
K795 Methylation
K795 Ubiquitination
T798 Phosphorylation
S801 Phosphorylation
K813 Ubiquitination
S819 Phosphorylation
Y823 Phosphorylation
K866 Ubiquitination
Y897 Phosphorylation
S911 Phosphorylation
S913 Phosphorylation
T918 Phosphorylation
Y919 Phosphorylation

Research Backgrounds

Function:

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with PCNA.

Family&Domains:

Belongs to the ATP-dependent DNA ligase family.

Research Fields

· Genetic Information Processing > Replication and repair > DNA replication.

· Genetic Information Processing > Replication and repair > Base excision repair.

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Genetic Information Processing > Replication and repair > Mismatch repair.

References

1). A Nucleophilicity-Engineered DNA Ligation Blockade Nanoradiosensitizer Induces Irreversible DNA Damage to Overcome Cancer Radioresistance. Advanced materials (Deerfield Beach, Fla.), 2024 (PubMed: 39246208) [IF=27.4]

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