CES1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat liver Mouse brain, using CES1 Antibody.

Product:	CES1 Antibody
Catalog:	DF6661
Description:	Rabbit polyclonal antibody to CES1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Dog
Mol.Wt.:	63kDa; 63kD(Calculated).
Uniprot:	P23141
RRID:	AB_2838623

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Dog(86%)

Clonality:

Polyclonal

Specificity:

CES1 Antibody detects endogenous levels of total CES1.

RRID:

AB_2838623
Cite Format: Affinity Biosciences Cat# DF6661, RRID:AB_2838623.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACAT; Acyl coenzyme A cholesterol acyltransferase; Acyl-coenzyme A:cholesterol acyltransferase; Brain carboxylesterase hBr1; Carboxyesterase ES-3; Carboxylesterase; Carboxylesterase 1 (monocyte/macrophage serine esterase 1); Carboxylesterase 1; Carboxylesterase 1 deficiency, included; Carboxylesterase 2, formerly; CE 1; CEH; Ces-1; CES1; CES2; CESDD1; Cholesterol ester hydrolase, neutral, macrophage-derived; Cholesteryl ester hydrolase; Cocaine carboxylesterase; EC 3.1.1.1; Egasyn; ES-HTEL; ES-x; Es22; EST1_HUMAN; Esterase 22; Esterase; hCE 1; HMSE; HMSE1; Liver carboxylesterase 1; Liver carboxylesterase 3; Methylumbelliferyl acetate deacetylase 1; MGC117365; MGC156521; Monocyte carboxylesterase deficiency, included; Monocyte esterase deficiency, included; Monocyte/macrophage serine esterase; PCE-1; pI 5.5 esterase; Proline-beta-naphthylamidase; REH; Retinyl ester hydrolase; Serine esterase 1; Ses-1; SES1; TGH; Triacylglycerol hydrolase;

Immunogens

Immunogen:

Uniprot:

P23141(EST1_HUMAN) >>Visit HPA database.

Gene(ID):

CES1(1066)

Expression:

P23141 EST1_HUMAN:

Expressed predominantly in liver with lower levels in heart and lung.

Description:

This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.

Sequence:

P23141 EST1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWLRAFILATLSASAAWGHPSSPPVVDTVHGKVLGKFVSLEGFAQPVAIFLGIPFAKPPLGPLRFTPPQPAEPWSFVKNATSYPPMCTQDPKAGQLLSELFTNRKENIPLKLSEDCLYLNIYTPADLTKKNRLPVMVWIHGGGLMVGAASTYDGLALAAHENVVVVTIQYRLGIWGFFSTGDEHSRGNWGHLDQVAALRWVQDNIASFGGNPGSVTIFGESAGGESVSVLVLSPLAKNLFHRAISESGVALTSVLVKKGDVKPLAEQIAITAGCKTTTSAVMVHCLRQKTEEELLETTLKMKFLSLDLQGDPRESQPLLGTVIDGMLLLKTPEELQAERNFHTVPYMVGINKQEFGWLIPMQLMSYPLSEGQLDQKTAMSLLWKSYPLVCIAKELIPEATEKYLGGTDDTVKKKDLFLDLIADVMFGVPSVIVARNHRDAGAPTYMYEFQYRPSFSSDMKPKTVIGDHGDELFSVFGAPFLKEGASEEEIRLSKMVMKFWANFARNGNPNGEGLPHWPEYNQKEGYLQIGANTQAAQKLKDKEVAFWTNLFAKKAVEKPPQTEHIEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23141 As Substrate

P23141

Site	PTM Type	Source
N79	N-Glycosylation	Uniprot
Y83	Phosphorylation	Uniprot
T88	Phosphorylation	Uniprot
S380	Phosphorylation	Uniprot
K393	Ubiquitination	Uniprot
K402	Acetylation	Uniprot
K402	Ubiquitination	Uniprot
Y403	Phosphorylation	Uniprot
T407	Phosphorylation	Uniprot
T444	Phosphorylation	Uniprot
S493	Phosphorylation	Uniprot
K538	Acetylation	Uniprot
K558	Acetylation	Uniprot

Research Backgrounds

P23141_EST1_HUMAN

Function:

Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate.

PTMs:

Contains sialic acid.

Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19.

Subcellular Location:

Endoplasmic reticulum lumen.

Tissue Specificity:

Expressed predominantly in liver with lower levels in heart and lung.

Subunit Structure:

Homotrimer and homohexamer. Binds to beta-glucuronidase.

Family&Domains:

Belongs to the type-B carboxylesterase/lipase family.

Research Fields

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

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CES1 Antibody - #DF6661