CD47 Antibody | Affinity Biosciences

WB
IHC
IF/ICC
Cites

1/7

Western blot analysis of extracts from various samples, using CD47 Antibody.

DF6649 at 1/100 staining human kidney by IHC-P.

DF6649 at 1/100 staining Rat brain tissue by IHC-P.

DF6649 at 1/100 staining Mouse brain tissue by IHC-P.

DF6649 staining Hela cells by IF/ICC.

Figure 1.

Product:	CD47 Antibody
Catalog:	DF6649
Description:	Rabbit polyclonal antibody to CD47
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Dog
Mol.Wt.:	35kDa,50kDa; 35kD(Calculated).
Uniprot:	Q08722
RRID:	AB_2838611

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Dog(100%)

Clonality:

Polyclonal

Specificity:

CD47 Antibody detects endogenous levels of total CD47.

RRID:

AB_2838611
Cite Format: Affinity Biosciences Cat# DF6649, RRID:AB_2838611.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Antigen identified by monoclonal antibody 1D8; Antigenic surface determinant protein OA3; CD 47; CD47; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); CD47 antigen; CD47 glycoprotein; CD47 molecule; CD47_HUMAN; IAP; Integrin Associated Protein; Integrin associated signal transducer; Integrin-associated protein; Leukocyte surface antigen CD47; MER 6; MER6; OA 3; OA3; OTTHUMP00000041152; OTTHUMP00000041153; Protein MER6; Rh related antigen; Surface antigen identified by monoclonal antibody 1D8;

Immunogens

Immunogen:

Uniprot:

Q08722(CD47_HUMAN) >>Visit HPA database.

Gene(ID):

CD47(961)

Expression:

Q08722 CD47_HUMAN:

Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Description:

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Sequence:

Q08722 CD47_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWPLVAALLLGSACCGSAQLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPNENILIVIFPIFAILLFWGQFGIKTLKYRSGGMDEKTIALLVAGLVITVIVIVGAILFVPGEYSLKNATGLGLIVTSTGILILLHYYVFSTAIGLTSFVIAILVIQVIAYILAVVGLSLCIAACIPMHGPLLISGLSILALAQLLGLVYMKFVASNQKTIQPPRKAVEEPLNAFKESKGMMNDE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Bovine

Sheep

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08722 As Substrate

Q08722

Site	PTM Type	Source
N23	N-Glycosylation	Uniprot
N50	N-Glycosylation	Uniprot
N73	N-Glycosylation	Uniprot
K74	Ubiquitination	Uniprot
K85	Ubiquitination	Uniprot
K93	Ubiquitination	Uniprot
K99	Ubiquitination	Uniprot
K102	Ubiquitination	Uniprot
N111	N-Glycosylation	Uniprot
K304	Ubiquitination	Uniprot
K314	Ubiquitination	Uniprot
S316	Phosphorylation	Uniprot
K317	Ubiquitination	Uniprot

Research Backgrounds

Q08722_CD47_HUMAN

Function:

Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain.

Subunit Structure:

Interacts with THBS1 and fibrinogen (By similarity). Monomer. Interacts with SIRPA, SIRPG, UBQLN1 and UBQLN2.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction. (View pathway)

References

1). Sensitive Activatable Nanoprobes for Real-time Ratiometric Magnetic Resonance Imaging of Reactive Oxygen Species and Ameliorating Inflammation In Vivo. ADVANCED MATERIALS, 2022 (PubMed: 35276751) [IF=30.0]

Application: WB Species: Mouse Sample:

Figure 1. TMSN@PM as an inflammation-disease theranostic. d) Western blot analysis of CD61, GPIbα, CD62P, and CD47 in Plt, PM, and TMSN.

2). Exercise-induced Musclin determines the fate of fibro-adipogenic progenitors to control muscle homeostasis. Cell stem cell, 2024 (PubMed: 38232727) [IF=23.9]

3). In situ MUC1-specific CAR engineering of tumor-supportive macrophages stimulates tumoricidal immunity against pancreatic adenocarcinoma. Nano Today, 2023 [IF=17.4]

4). Microvesicle-inspired oxygen-delivering nanosystem potentiates radiotherapy-mediated modulation of tumor stroma and antitumor immunity. Biomaterials, 2022 (PubMed: 36257260) [IF=14.0]

5). Arsenic retention in erythrocytes and excessive erythrophagocytosis is related to low selenium status by impaired redox homeostasis. Redox Biology, 2022 (PubMed: 35500533) [IF=11.4]

6). Extracellular vesicle-mediated co-delivery of TRAIL and dinaciclib for targeted therapy of resistant tumors. Biomaterials Science, 2022 (PubMed: 35170591) [IF=6.6]

7). Optimization of lipid assisted polymeric nanoparticles for siRNA delivery and cancer immunotherapy. Biomaterials science, 2024 (PubMed: 38469870) [IF=6.6]

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CD47 Antibody - #DF6649