SP-C Antibody - #DF6647
(12)
(6)
| Product: | SP-C Antibody |
| Catalog: | DF6647 |
| Description: | Rabbit polyclonal antibody to SP-C |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Bovine, Horse, Rabbit |
| Mol.Wt.: | 21kDa; 21kD(Calculated). |
| Uniprot: | P11686 |
| RRID: | AB_2838609 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Rabbit(92%)
Clonality:
Polyclonal
Specificity:
SP-C Antibody detects endogenous levels of total SP-C.
RRID:
AB_2838609
Cite Format: Affinity Biosciences Cat# DF6647, RRID:AB_2838609.
Cite Format: Affinity Biosciences Cat# DF6647, RRID:AB_2838609.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
BRICD6; BRICHOS domain containing 6; PSP C; PSPC; PSPC_HUMAN; Pulmonary surfactant apoprotein 2; Pulmonary surfactant apoprotein PSP C; pulmonary surfactant apoprotein-2 SP-C; Pulmonary surfactant associated protein C; Pulmonary surfactant associated proteolipid SPL pVal; Pulmonary surfactant associated proteolipid SPL(Val); Pulmonary surfactant protein SP5; Pulmonary surfactant-associated protein C; Pulmonary surfactant-associated proteolipid SPL(Val); SFTP 2; SFTP2; SFTPC; SFTPC surfactant pulmonary associated protein C; SMDP2; SP 5; SP C; SP-C; SP5; SPC; Surfactant associated protein pulmonary 2; Surfactant protein c; Surfactant proteolipid SPL-pVal; Surfactant pulmonary associated protein C;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
This gene encodes the pulmonary-associated surfactant protein C (SPC), an extremely hydrophobic surfactant protein essential for lung function and homeostasis after birth. Pulmonary surfactant is a surface-active lipoprotein complex composed of 90% lipids and 10% proteins which include plasma proteins and apolipoproteins SPA, SPB, SPC and SPD. The surfactant is secreted by the alveolar cells of the lung and maintains the stability of pulmonary tissue by reducing the surface tension of fluids that coat the lung. Multiple mutations in this gene have been identified, which cause pulmonary surfactant metabolism dysfunction type 2, also called pulmonary alveolar proteinosis due to surfactant protein C deficiency, and are associated with interstitial lung disease in older infants, children, and adults. Alternatively spliced transcript variants encoding different protein isoforms have been identified.
Sequence:
- P11686 PSPC_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVGSKEVLMESPPDYSAAPRGRFGIPCCPVHLKRLLIVVVVVVLIVVVIVGALLMGLHMSQKHTEMVLEMSIGAPEAQQRLALSEHLVTTATFSIGSTGLVVYDYQQLLIAYKPAPGTCCYIMKIAPESIPSLEALNRKVHNFQMECSLQAKPAVPTSKLGQAEGRDAGSAPSGGDPAFLGMAVNTLCGEVPLYYI
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
100
Bovine
100
Rabbit
92
Xenopus
71
Pig
0
Sheep
0
Dog
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P11686 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| Y16 | Phosphorylation | Uniprot | |
| T187 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Subcellular Location:
Secreted>Extracellular space>Surface film.
References
1). Extracellular Vesicle ITGAM and ITGB2 Mediate Severe Acute Pancreatitis-Related Acute Lung Injury. ACS Nano, 2023
(PubMed: 37022097)
[IF=17.1]
2). Impaired autophagy-accelerated senescence of alveolar type II epithelial cells drives pulmonary fibrosis induced by single-walled carbon nanotubes. Journal of Nanobiotechnology, 2023
(PubMed: 36849924)
[IF=10.2]
Application: WB Species: Mouse Sample: lung tissues
Fig. 1 Single-walled carbon nanotubes (SWCNTs) induced pulmonary fibrosis and senescence of AECIIs in mouse lung tissues. Mice were exposed to 40 μg SWCNT by intratracheal instillation, then lung tissues were collected on days 3, 7 and 28. A Scheme of workflow for evaluation of cellular senescence and pulmonary fibrosis induced by SWCNTs in vivo. B–D Western Blotting analysis of p21 and p16 protein expressions (n = 3) in lung tissues. Contents of TGF-β (E) and PAI-1 (F) in bronchoalveolar lavage fluid (BALF) quantified by ELISA (n = 5). HE staining (G) and Masson’s trichrome staining (H) of mouse lung tissues (200×). I The semiquantitative Ashcroft scores for the severity of pulmonary fibrosis (n = 4). J The hydroxyproline (HYP) level (n = 4) in lung tissues of mice. K Immunohistochemistry (IHC) of COL I expression (n = 5) in lung tissues of mice. L, M The correlation of hydroxyproline contents in mice lung tissues and senescence-associated secretory phenotype (SASP) factors (TGF-β and PAI-1) in BALF. N Immunostaining for p16 (a senescence-related marker) and SP-C (an AECIIs marker) from SWCNTs-exposed lung tissues of mice on day 28 was detected by immunofluorescence (IF) (400×). *P
3). Insights into the mechanism of action of pterostilbene against influenza A virus-induced acute lung injury. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024
(PubMed: 38583346)
[IF=7.9]
4). Novel genome-wide DNA methylation profiling reveals distinct epigenetic landscape, prognostic model and cellular composition of early-stage lung adenocarcinoma. Journal of translational medicine, 2024
(PubMed: 38711158)
[IF=7.4]
Application: IHC Species: Human Sample:
Fig. 5 UXM analysis identified various cell populations in EM-seq and were verified by Immunohistochemistry (A) Cell proportion score between tumor (red) and normal (green) tissue analyzed by UXM. (B) The cellular composition of each tumor and normal tissue identified by UXM. (C) Representative immunohistochemistry image shows that CD3、CD19、SP-C were highly expressed in NSCLC tumor tissues (C-E); CD31were highly expressed in normal lung tissue (F); CD56 were weakly expressed in normal lung tissue (G).
5). Keratin 15 protects against cigarette smoke-induced epithelial mesenchymal transformation by MMP-9. Respiratory research, 2023
(PubMed: 38007424)
[IF=5.8]
Application: IF/ICC Species: Mouse Sample:
Fig. 9 Krt15+ cells transform into AT2 cells to protect alveoli. A Immunofluorescence staining of lung tissue with Krt15 (Red) and Sftpc (Green). B The ratio of Krt15+Sftpc+ cells/Total cells. C Staining-intensity profiles showing signals from three fluorescent channels. D The ratio of TUNEL+ cells/Total cells. E TUNEL staining. N = 3 in each group.*p
6). Establishment of Intestinal Organoid from Rousettus leschenaultii and the Susceptibility to Bat-Associated Viruses, SARS-CoV-2 and Pteropine Orthoreovirus. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021
(PubMed: 34639103)
[IF=5.6]
7). Clevudine attenuates bleomycin-induced early pulmonary fibrosis via regulating M2 macrophage polarization. International Immunopharmacology, 2021
(PubMed: 34700113)
[IF=5.6]
8). Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats. JOURNAL OF ETHNOPHARMACOLOGY, 2023
(PubMed: 36535334)
[IF=5.4]
9). NLRP3 inflammasome mediates abnormal epithelial regeneration and distal lung remodeling in silica‑induced lung fibrosis. International journal of molecular medicine, 2024
(PubMed: 38240085)
[IF=5.4]
10). Rosmarinic acid treatment protects against lethal H1N1 virus-mediated inflammation and lung injury by promoting activation of the h-PGDS-PGD2-HO-1 signal axis. Chinese medicine, 2023
(PubMed: 37891648)
[IF=4.9]
Application: IF/ICC Species: Mouse Sample:
Fig. 2 Effects of RosA on the H1N1 virus-triggered pro-inflammatory response. A Representative immunofluorescence images of IL-6, IL-8 (pink) and TNF-α, MCP-1 (red) in lung SpC+ (green) alveolar epithelial cells. B Quantitative analysis of fluorescence intensities for IL-6, TNF-α, IL-8 and MCP-1 in SpC+ alveolar epithelial cells. C Levels of pro-inflammatory mediators (IL-6, MCP-1, RANTES and TNF-α) in the lung homogenates were determined by Luminex assay. D Levels of pro-inflammatory mediators (IL-6, IL-8, IP-10, MCP-1, RANTES and TNF-α) were determined by Luminex assay.
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