IL-10R alpha Antibody - #DF6643

(2)   (4)  
Product: IL-10R alpha Antibody
Catalog: DF6643
Description: Rabbit polyclonal antibody to IL-10R alpha
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 63kDa; 63kD(Calculated).
Uniprot: Q13651
RRID: AB_2838605

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Horse(86%), Rabbit(86%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
IL-10R alpha Antibody detects endogenous levels of total IL-10R alpha.
RRID:
AB_2838605
Cite Format: Affinity Biosciences Cat# DF6643, RRID:AB_2838605.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD210; CDw210a; CDw210a antigen; HIL 10R; HIL10R; I10R1_HUMAN; IL 10R 1; IL 10R A; IL 10R; IL 10R1; IL-10 receptor subunit alpha; IL-10R subunit 1; IL-10R subunit alpha; IL-10R1; IL-10RA; IL10R; IL10R1; IL10RA; Interleukin 10 receptor alpha; Interleukin 10 receptor alpha chain; Interleukin-10 receptor subunit 1; Interleukin-10 receptor subunit alpha;

Immunogens

Immunogen:
Uniprot:

Q13651(I10R1_HUMAN) >>Visit HPA database.

Gene(ID):
IL10RA(3587)
Expression:
Q13651 I10R1_HUMAN:

Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells.

Description:
The protein encoded by this gene is a receptor for interleukin 10. This protein is structurally related to interferon receptors. It has been shown to mediate the immunosuppressive signal of interleukin 10, and thus inhibits the synthesis of proinflammatory cytokines. This receptor is reported to promote survival of progenitor myeloid cells through the insulin receptor substrate-2/PI 3-kinase/AKT pathway. Activation of this receptor leads to tyrosine phosphorylation of JAK1 and TYK2 kinases. Two transcript variants, one protein-coding and the other not protein-coding, have been found for this gene. [provided by RefSeq, Jan 2009]
Sequence:
MLPCLVVLLAALLSLRLGSDAHGTELPSPPSVWFEAEFFHHILHWTPIPNQSESTCYEVALLRYGIESWNSISNCSQTLSYDLTAVTLDLYHSNGYRARVRAVDGSRHSNWTVTNTRFSVDEVTLTVGSVNLEIHNGFILGKIQLPRPKMAPANDTYESIFSHFREYEIAIRKVPGNFTFTHKKVKHENFSLLTSGEVGEFCVQVKPSVASRSNKGMWSKEECISLTRQYFTVTNVIIFFAFVLLLSGALAYCLALQLYVRRRKKLPSVLLFKKPSPFIFISQRPSPETQDTIHPLDEEAFLKVSPELKNLDLHGSTDSGFGSTKPSLQTEEPQFLLPDPHPQADRTLGNREPPVLGDSCSSGSSNSTDSGICLQEPSLSPSTGPTWEQQVGSNSRGQDDSGIDLVQNSEGRAGDTQGGSALGHHSPPEPEVPGEEDPAAVAFQGYLRQTRCAEEKATKTGCLEEESPLTDGLGPKFGRCLVDEAGLHPPALAKGYLKQDPLEMTLASSGAPTGQWNQPTEEWSLLALSSCSDLGISDWSFAHDLAPLGCVAAPGGLLGSFNSDLVTLPLISSLQSSE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Chicken
100
Horse
86
Rabbit
86
Bovine
71
Sheep
71
Xenopus
71
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13651 As Substrate

Site PTM Type Enzyme
K183 Acetylation
S268 Phosphorylation
S319 Phosphorylation
S323 Phosphorylation
S370 Phosphorylation
Y446 Phosphorylation P23458 (JAK1) , P29597 (TYK2)
Y496 Phosphorylation P29597 (TYK2) , P23458 (JAK1)

Research Backgrounds

Function:

Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3. Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy.

PTMs:

Phosphorylated. Phosphorylation of the cytoplasmic tail induced STAT3 activation.

Ubiquitinated by BTRC; ubiquitination leads to endocytosis and subsequent degradation of IL10RA.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Primarily expressed in hematopoetic cells including B-cells, T-cells, NK cells, monocytes and macrophages. Not expressed in non-hematopoetic cells such as fibroblasts or endothelial cells.

Subunit Structure:

Interacts with IL10. Interacts with IL10RB. Interacts (via its cytoplasmic domain) with JAK1 (via N-terminus). Interacts with BTRC; this interaction leads to IL10RA ubiquitination and subsequent degradation. Interacts with STAT3 (By similarity).

(Microbial infection) Interacts with human cytomegalovirus protein IL10.

(Microbial infection) Interacts with Epstein-Barr virus protein IL10.

Family&Domains:

Belongs to the type II cytokine receptor family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Toxoplasmosis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

References

1). Bioinformatics Analysis for Identification of Key Genes in Salivary Gland and the Potential of a Combination of Biomarkers for the Diagnosis of SS. Journal of Inflammation Research, 2021 (PubMed: 34475773) [IF=4.5]

Application: IF/ICC    Species: Human    Sample: gland tissue

Figure 5 H&E and immunohistochemical analysis (A) of STAT1, MNDA, IL10RA, and CCR1 proteins in minor salivary gland biopsy (MSGB) staining from 14 SS patients and 14 non-SS patients (magnification: ×200 for H&E staining and ×200 for IHC). Blue marks the nucleus, and brown marks the target protein. The bar graph indicated medians with interquartile ranges of IOD value (B). ***P<0.001.

Application: IHC    Species: human    Sample: Labial Gland Tissues

Figure 5| H&E and immunohistochemical analysis (A) of STAT1, MNDA, IL10RA, and CCR1 proteins in minor salivary gland biopsy (MSGB) staining from 14 SS patients and 14 non-SS patients (magnification: ×200 for H&E staining and ×200 for IHC). Blue marks the nucleus, and brown marks the target protein. The bar graph indicated medians with interquartile ranges of IOD value (B). ***P<0.001.
2). Identification of Biomarkers Related to Regulatory T Cell Infiltration in Oral Squamous Cell Carcinoma Based on Integrated Bioinformatics Analysis. International Journal of General Medicine, 2022 (PubMed: 35264874) [IF=2.3]

Application: IHC    Species: human    Sample: oral mucosa

Figure 4| LCK, TNFRSF1B and IL10RA was upregulated in OSCC patients and was associated with poor prognosis. (A) Immunohistochemical staining of tissue microarrays. As clinical staging progressed, staining for IL10RA, LCK and TNFRSF1B ranged from normal tissue to weak to moderate to strong.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.