THPO Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using THPO Antibody.

Product:	THPO Antibody
Catalog:	DF6640
Description:	Rabbit polyclonal antibody to THPO
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Horse, Sheep
Mol.Wt.:	38kDa; 38kD(Calculated).
Uniprot:	P40225
RRID:	AB_2838602

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Horse(83%), Sheep(83%)

Clonality:

Polyclonal

Specificity:

THPO Antibody detects endogenous levels of total THPO.

RRID:

AB_2838602
Cite Format: Affinity Biosciences Cat# DF6640, RRID:AB_2838602.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

C mpl ligand; C-mpl ligand; Megakaryocyte colony stimulating factor; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; Megakaryocyte stimulating factor; MGC163194; MGDF; MKCSF; ML; MPL ligand; MPLLG; Myeloproliferative leukemia virus oncogene ligand; Prepro thrombopoietin; THCYT1; THPO; Thrombopoietin; Thrombopoietin nirs variant 1; TPO; TPO_HUMAN;

Immunogens

Immunogen:

Uniprot:

P40225(TPO_HUMAN) >>Visit HPA database.

Gene(ID):

THPO(7066)

Description:

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternate splicing results in multiple transcript variants of this gene.[provided by RefSeq, Jun 2012]

Sequence:

P40225 TPO_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MELTELLLVVMLLLTARLTLSSPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLGEWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQVRLLLGALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGGSTLCVRRAPPTTAVPSRTSLVLTLNELPNRTSGLLETNFTASARTTGSGLLKWQQGFRAKIPGLLNQTSRSLDQIPGYLNRIHELLNGTRGLFPGPSRRTLGAPDISSGTSDTGSLPPNLQPGYSPSPTHPPTGQYTLFPLPPTLPTPVVQLHPLLPDPSAPTPTPTSPLLNTSYTHSQNLSQEG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Sheep

Rabbit

Dog

Pig

Bovine

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40225 As Substrate

P40225

Site	PTM Type	Source
S22	O-Glycosylation	Uniprot
S34	Phosphorylation	Uniprot
T58	O-Glycosylation	Uniprot
T58	Phosphorylation	Uniprot
S68	Phosphorylation	Uniprot
T79	Phosphorylation	Uniprot
T131	O-Glycosylation	Uniprot
T179	O-Glycosylation	Uniprot
T180	O-Glycosylation	Uniprot
S184	O-Glycosylation	Uniprot
N197	N-Glycosylation	Uniprot
T205	Phosphorylation	Uniprot
N206	N-Glycosylation	Uniprot
T213	O-Glycosylation	Uniprot
S216	Phosphorylation	Uniprot
N234	N-Glycosylation	Uniprot
N255	N-Glycosylation	Uniprot
S265	O-Glycosylation	Uniprot

Research Backgrounds

P40225_TPO_HUMAN

Function:

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.

Subcellular Location:

Secreted.

Family&Domains:

Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.

Belongs to the EPO/TPO family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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THPO Antibody - #DF6640