SDC2 Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat heart, using SDC2 Antibody.

Product:	SDC2 Antibody
Catalog:	DF6630
Description:	Rabbit polyclonal antibody to SDC2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	22kDa; 22kD(Calculated).
Uniprot:	P34741
RRID:	AB_2838592

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

SDC2 Antibody detects endogenous levels of total SDC2.

RRID:

AB_2838592
Cite Format: Affinity Biosciences Cat# DF6630, RRID:AB_2838592.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD362; Fibroglycan; Heparan sulfate proteoglycan core protein; HSPG; SDC2; SDC2_HUMAN; SYND2; Syndecan-2;

Immunogens

Immunogen:

Uniprot:

P34741(SDC2_HUMAN) >>Visit HPA database.

Gene(ID):

SDC2(6383)

Description:

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-2 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-2 expression has been detected in several different tumor types. [provided by RefSeq, Jul 2008]

Sequence:

P34741 SDC2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRRAWILLTLGLVACVSAESRAELTSDKDMYLDNSSIEEASGVYPIDDDDYASASGSGADEDVESPELTTSRPLPKILLTSAAPKVETTTLNIQNKIPAQTKSPEETDKEKVHLSDSERKMDPAEEDTNVYTEKHSDSLFKRTEVLAAVIAGGVIGFLFAIFLILLLVYRMRKKDEGSYDLGERKPSSAAYQKAPTKEFYA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P34741 As Substrate

P34741

Site	PTM Type	Enzyme	Source
S41	Phosphorylation		Uniprot
T80	O-Glycosylation		Uniprot
S81	O-Glycosylation		Uniprot
T88	O-Glycosylation		Uniprot
T89	O-Glycosylation		Uniprot
T90	O-Glycosylation		Uniprot
T101	O-Glycosylation		Uniprot
T107	O-Glycosylation		Uniprot
S115	Phosphorylation		Uniprot
K174	Ubiquitination		Uniprot
Y179	Phosphorylation	P12931 (SRC)	Uniprot
R184	Methylation		Uniprot
K185	Ubiquitination		Uniprot
S187	Phosphorylation	P05129 (PRKCG) , P17252 (PRKCA) , P05771 (PRKCB)	Uniprot
S188	Phosphorylation	P05771 (PRKCB) , P05129 (PRKCG) , P17252 (PRKCA)	Uniprot
Y191	Phosphorylation		Uniprot
K193	Ubiquitination		Uniprot
T196	Phosphorylation		Uniprot
K197	Ubiquitination		Uniprot
Y200	Phosphorylation		Uniprot

Research Backgrounds

P34741_SDC2_HUMAN

Function:

Cell surface proteoglycan that bears heparan sulfate. Regulates dendritic arbor morphogenesis (By similarity).

PTMs:

O-glycosylated with core 1 or possibly core 8 glycans. Contains heparan sulfate.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Subunit Structure:

Interacts (via cytoplasmic domain) with SARM1 (By similarity). Forms a complex with SDCBP and PDCD6IP.

Family&Domains:

Belongs to the syndecan proteoglycan family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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SDC2 Antibody - #DF6630