ALDH1A1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from A549 cells(UV treatment), using ALDH1A1 Antibody.

Figure 2 Emo enhances inhibitory effects of GEM on self-renewal of SW1990/GZ cells.

Figure 7 Growth of stem cells and EMT progression in SW1990/GZ xenograft model is inhibited by Emo by inactivating Snail.

Product:	ALDH1A1 Antibody
Catalog:	DF6625
Description:	Rabbit polyclonal antibody to ALDH1A1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	55kDa; 55kD(Calculated).
Uniprot:	P00352
RRID:	AB_2838587

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(100%), Dog(82%), Chicken(82%)

Clonality:

Polyclonal

Specificity:

ALDH1A1 Antibody detects endogenous levels of total ALDH1A1.

RRID:

AB_2838587
Cite Format: Affinity Biosciences Cat# DF6625, RRID:AB_2838587.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Acetaldehyde dehydrogenase 1; AHD2; AL1A1_HUMAN; ALDC; Aldehyde dehydrogenase 1 family member A1; Aldehyde dehydrogenase 1 soluble; Aldehyde dehydrogenase 1A1; Aldehyde dehydrogenase; Aldehyde dehydrogenase cytosolic; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase liver cytosolic; ALDH 1; ALDH 1A1; ALDH class 1; ALDH, liver cytosolic; ALDH-E1; ALDH1 A1; ALDH1; ALDH11; ALDH1A1; ALHDII; cytosolic; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; HEL-S-53e; MGC2318; PUMB1; RALDH 1; RalDH1; Retinal dehydrogenase 1;

Immunogens

Immunogen:

Uniprot:

P00352(AL1A1_HUMAN) >>Visit HPA database.

Gene(ID):

ALDH1A1(216)

Description:

The aldehyde dehydrogenase family is a large group of enzymes that oxidize aldehydes formed through metabolic processes to their carboxylic acids (1). ALDH1A1 is a liver cytosolic isoform of acetaldehyde dehydrogenase and is involved in the major pathway of alcohol metabolism along with alcohol dehydrogenase (2). ALDH1A1 is also known as retinal dehydrogenase 1 and is involved in retinol metabolism, converting retinol to retinoic acid (3). Recent studies suggest that control of retinoid signaling through ALDH1A1 may influence hematopoietic stem cell differentiation (4). There has been recent interest in ALDH1 isoforms as predictive biomarkers in disease. Several studies have suggested that ALDH1A1 is a potential marker for cancer stem cells and chemoresistance in several tumor types, such as melanoma (5), lung cancer (6), glioblastoma (7).

Sequence:

P00352 AL1A1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSSGTPDLPVLLTDLKIQYTKIFINNEWHDSVSGKKFPVFNPATEEELCQVEEGDKEDVDKAVKAARQAFQIGSPWRTMDASERGRLLYKLADLIERDRLLLATMESMNGGKLYSNAYLNDLAGCIKTLRYCAGWADKIQGRTIPIDGNFFTYTRHEPIGVCGQIIPWNFPLVMLIWKIGPALSCGNTVVVKPAEQTPLTALHVASLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDIDKVAFTGSTEVGKLIKEAAGKSNLKRVTLELGGKSPCIVLADADLDNAVEFAHHGVFYHQGQCCIAASRIFVEESIYDEFVRRSVERAKKYILGNPLTPGVTQGPQIDKEQYDKILDLIESGKKEGAKLECGGGPWGNKGYFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSLDDVIKRANNTFYGLSAGVFTKDIDKAITISSALQAGTVWVNCYGVVSAQCPFGGFKMSGNGRELGEYGFHEYTEVKTVTVKISQKNS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Horse

100

Pig

Bovine

Sheep

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00352 As Substrate

P00352

Site	PTM Type	Source
S2	Acetylation	Uniprot
S2	Phosphorylation	Uniprot
T6	Phosphorylation	Uniprot
K22	Acetylation	Uniprot
K65	Acetylation	Uniprot
R68	Methylation	Uniprot
R85	Methylation	Uniprot
K91	Acetylation	Uniprot
T105	Phosphorylation	Uniprot
Y119	Phosphorylation	Uniprot
K128	Acetylation	Uniprot
K128	Ubiquitination	Uniprot
K139	Acetylation	Uniprot
K139	Ubiquitination	Uniprot
T144	Phosphorylation	Uniprot
Y154	Phosphorylation	Uniprot
K252	Acetylation	Uniprot
T267	Phosphorylation	Uniprot
S314	Phosphorylation	Uniprot
Y316	Phosphorylation	Uniprot
T337	Phosphorylation	Uniprot
T341	Phosphorylation	Uniprot
K353	Acetylation	Uniprot
S360	Phosphorylation	Uniprot
K362	Acetylation	Uniprot
K367	Acetylation	Uniprot
K398	Ubiquitination	Uniprot
K410	Acetylation	Uniprot
K412	Acetylation	Uniprot
K412	Ubiquitination	Uniprot
S413	Phosphorylation	Uniprot
K419	Acetylation	Uniprot
T424	Phosphorylation	Uniprot
Y426	Phosphorylation	Uniprot
S429	Phosphorylation	Uniprot
K435	Acetylation	Uniprot
K435	Ubiquitination	Uniprot
T442	Phosphorylation	Uniprot
Y481	Phosphorylation	Uniprot
Y486	Phosphorylation	Uniprot
K490	Ubiquitination	Uniprot
T493	Phosphorylation	Uniprot
K495	Acetylation	Uniprot
K495	Ubiquitination	Uniprot

Research Backgrounds

P00352_AL1A1_HUMAN

Function:

Can convert/oxidize retinaldehyde to retinoic acid. Binds free retinal and cellular retinol-binding protein-bound retinal (By similarity). May have a broader specificity and oxidize other aldehydes in vivo.

PTMs:

The N-terminus is blocked most probably by acetylation.

Subcellular Location:

Cytoplasm>Cytosol.

Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the aldehyde dehydrogenase family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition. Experimental and Therapeutic Medicine (PubMed: 36545274) [IF=2.7]

Application: WB Species: Human Sample: SW1990/GZ cells

Figure 2 Emo enhances inhibitory effects of GEM on self-renewal of SW1990/GZ cells. (A) Morphology of cells observed under an inverted light microscope (scale bar, 100 µm). (B) The ultrastructure of cells observed under a transmission electron microscope (x10,000 magnification). (C) Cell sphere-forming rate evaluated using the sphere formation assay (x100 magnification). (D) Number of spheres measured using the colony-formation assay (x200 magnification). (E) Expression levels of CD44, ALDH1, Nanog and Snail determined using western blotting assays. *P

Application: IHC Species: Human Sample: SW1990/GZ cells

Figure 7 Growth of stem cells and EMT progression in SW1990/GZ xenograft model is inhibited by Emo by inactivating Snail. (A) Expression levels of CD44, ALDH1, Nanog and Snail as determined via western blotting. (B) Expression level of CD44, ALDH1, Nanog and Snail in tumor tissues measured via immunohistochemistry (x200 magnification). (C) Expression levels of vimentin and E-cadherin as determined via western blotting. (D) Expression levels of vimentin and E-cadherin in tumor tissues measured via immunohistochemistry (x200 magnification). *P

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ALDH1A1 Antibody - #DF6625