XRCC2 Antibody | Affinity Biosciences

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Western blot analysis of Hela whole cell lysates, using XRCC2 Antibody.

DF6624 at 1/100 staining Mouse spleen tissue by IHC-P.

Product:	XRCC2 Antibody
Catalog:	DF6624
Description:	Rabbit polyclonal antibody to XRCC2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	32kDa; 32kD(Calculated).
Uniprot:	O43543
RRID:	AB_2838586

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

XRCC2 Antibody detects endogenous levels of total XRCC2.

RRID:

AB_2838586
Cite Format: Affinity Biosciences Cat# DF6624, RRID:AB_2838586.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DKFZp781P0919; DNA repair protein XRCC2; X ray repair complementing defective repair in Chinese hamster cells 2; X ray repair cross complementing protein 2; X-ray repair cross-complementing protein 2; Xrcc2; XRCC2_HUMAN;

Immunogens

Immunogen:

Uniprot:

O43543(XRCC2_HUMAN) >>Visit HPA database.

Gene(ID):

XRCC2(7516)

Description:

This gene encodes a member of the RecA/Rad51-related protein family that participates in homologous recombination to maintain chromosome stability and repair DNA damage. This gene is involved in the repair of DNA double-strand breaks by homologous recombination and it functionally complements Chinese hamster irs1, a repair-deficient mutant that exhibits hypersensitivity to a number of different DNA-damaging agents. [provided by RefSeq, Jul 2008]

Sequence:

O43543 XRCC2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MCSAFHRAESGTELLARLEGRSSLKEIEPNLFADEDSPVHGDILEFHGPEGTGKTEMLYHLTARCILPKSEGGLEVEVLFIDTDYHFDMLRLVTILEHRLSQSSEEIIKYCLGRFFLVYCSSSTHLLLTLYSLESMFCSHPSLCLLILDSLSAFYWIDRVNGGESVNLQESTLRKCSQCLEKLVNDYRLVLFATTQTIMQKASSSSEEPSHASRRLCDVDIDYRPYLCKAWQQLVKHRMFFSKQDDSQSSNQFSLVSRCLKSNSLKKHFFIIGESGVEFC

PTMs - O43543 As Substrate

O43543

Site	PTM Type	Source
S10	Phosphorylation	Uniprot
T12	Phosphorylation	Uniprot
K25	Ubiquitination	Uniprot
Y59	Phosphorylation	Uniprot
K236	Ubiquitination	Uniprot
K243	Ubiquitination	Uniprot
S247	Phosphorylation	Uniprot
S249	Phosphorylation	Uniprot
K261	Ubiquitination	Uniprot

Research Backgrounds

O43543_XRCC2_HUMAN

Function:

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA.

Subcellular Location:

Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Subunit Structure:

Interacts with RAD51D. Part of the BCDX2 complex consisting of RAD51B, RAD51C, RAD51D and XRCC2; the complex has a ring-like structure arranged into a flat disc around a central channel. In the absence of DNA, the BCDX2 subcomplex XRCC2:RAD51D formed a multimeric ring structure; in the presence of single-stranded DNA it formed a filamentous structure with the ssDNA.

Family&Domains:

Belongs to the RecA family. RAD51 subfamily.

Research Fields

· Genetic Information Processing > Replication and repair > Homologous recombination.

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XRCC2 Antibody - #DF6624