RGS2 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from Rat lung, using RGS2 Antibody.

DF6621 at 1/100 staining Mouse lung tissue by IHC-P.

Product:	RGS2 Antibody
Catalog:	DF6621
Description:	Rabbit polyclonal antibody to RGS2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	24kDa; 24kD(Calculated).
Uniprot:	P41220
RRID:	AB_2838583

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%)

Clonality:

Polyclonal

Specificity:

RGS2 Antibody detects endogenous levels of total RGS2.

RRID:

AB_2838583
Cite Format: Affinity Biosciences Cat# DF6621, RRID:AB_2838583.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Basic helix-loop-helix phosphoprotein G0S8; Cell growth inhibiting protein 31; Cell growth-inhibiting gene 31 protein; G0 to G1 switch regulatory 8 24kD; G0/G1 switch regulatory protein 8; G0S8; GOS8; OTTHUMP00000060765; Regulator of G protein signaling 2; Regulator of G protein signalling 2 24kD; Regulator of G-protein signaling 2; Regulators of G protein signaling XRGSVI; RGS 2; RGS2; RGS2_HUMAN;

Immunogens

Immunogen:

Uniprot:

P41220(RGS2_HUMAN) >>Visit HPA database.

Gene(ID):

RGS2(5997)

Expression:

P41220 RGS2_HUMAN:

Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).

Description:

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 2 belongs to this family. The protein acts as a mediator of myeloid differentiation and may play a role in leukemogenesis. [provided by RefSeq, Aug 2009]

Sequence:

P41220 RGS2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQSAMFLAVQHDCRPMDKSAGSGHKSEEKREKMKRTLLKDWKTRLSYFLQNSSTPGKPKTGKKSKQQAFIKPSPEEAQLWSEAFDELLASKYGLAAFRAFLKSEFCEENIEFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKEINIDFQTKTLIAQNIQEATSGCFTTAQKRVYSLMENNSYPRFLESEFYQDLCKKPQITTEPHAT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Pig

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41220 As Substrate

P41220

Site	PTM Type	Enzyme	Source
K39	Ubiquitination		Uniprot
S46	Phosphorylation	Q13976 (PRKG1)	Uniprot
T54	Phosphorylation		Uniprot
K57	Ubiquitination		Uniprot
S64	Phosphorylation	Q13976 (PRKG1)	Uniprot
K71	Ubiquitination		Uniprot
K121	Acetylation		Uniprot
K123	Acetylation		Uniprot
K127	Ubiquitination		Uniprot
K134	Ubiquitination		Uniprot
K142	Ubiquitination		Uniprot
K146	Ubiquitination		Uniprot
K175	Ubiquitination		Uniprot
Y178	Phosphorylation		Uniprot
S179	Phosphorylation		Uniprot
K201	Ubiquitination		Uniprot

Research Backgrounds

P41220_RGS2_HUMAN

Function:

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. It is involved in the negative regulation of the angiotensin-activated signaling pathway. Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein.

PTMs:

Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.

Subcellular Location:

Cell membrane. Cytoplasm. Nucleus>Nucleolus.

Cell membrane. Cytoplasm. Nucleus>Nucleolus.

Cell membrane. Cytoplasm. Nucleus>Nucleolus.

Cell membrane. Mitochondrion.

Tissue Specificity:

Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).

Subunit Structure:

Interacts with GNAQ. Does not interact with GNAI1 and GNAI3. Interacts with EIF2B5. Interacts with PRKG1 (isoform alpha).

Research Fields

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Organismal Systems > Sensory system > Olfactory transduction.

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

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Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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RGS2 Antibody - #DF6621