DCK Antibody - #DF6620

(2)  
Product: DCK Antibody
Catalog: DF6620
Description: Rabbit polyclonal antibody to DCK
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 31kDa; 31kD(Calculated).
Uniprot: P27707
RRID: AB_2838582

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(83%), Sheep(100%), Rabbit(92%), Dog(92%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
DCK Antibody detects endogenous levels of total DCK.
RRID:
AB_2838582
Cite Format: Affinity Biosciences Cat# DF6620, RRID:AB_2838582.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

dCK; DCK protein; DCK_HUMAN; Deoxycytidine kinase; Deoxynucleoside kinase; EC 2.7.1; EC 2.7.1.74; MGC117410; MGC138632; OTTHUMP00000219118; OTTHUMP00000219119;

Immunogens

Immunogen:
Uniprot:

P27707(DCK_HUMAN) >>Visit HPA database.

Gene(ID):
DCK(1633)
Description:
Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443). This antibody is specific to DCK.
Sequence:
MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Dog
92
Rabbit
92
Horse
83
Chicken
83
Zebrafish
50
Pig
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P27707 As Substrate

Site PTM Type Enzyme
T3 Phosphorylation
S8 Phosphorylation
C9 S-Nitrosylation
S11 Phosphorylation P48730 (CSNK1D)
S13 Phosphorylation
S15 Phosphorylation P48730 (CSNK1D)
S16 Phosphorylation
T19 Phosphorylation
K23 Ubiquitination
K34 Ubiquitination
S35 Phosphorylation
K42 Ubiquitination
S63 Phosphorylation
T64 Phosphorylation
T72 Phosphorylation P48730 (CSNK1D)
S74 Phosphorylation Q13535 (ATR) , P48730 (CSNK1D) , Q13315 (ATM)
K76 Ubiquitination
K88 Acetylation
K88 Ubiquitination
K115 Ubiquitination
K117 Ubiquitination
K121 Ubiquitination
Y190 Phosphorylation
Y204 Phosphorylation
K211 Ubiquitination
K222 Ubiquitination
K243 Ubiquitination
K245 Ubiquitination
K252 Acetylation
K252 Ubiquitination
K254 Ubiquitination

Research Backgrounds

Function:

Required for the phosphorylation of the deoxyribonucleosides deoxycytidine (dC), deoxyguanosine (dG) and deoxyadenosine (dA). Has broad substrate specificity, and does not display selectivity based on the chirality of the substrate. It is also an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents.

PTMs:

Phosphorylated and activated in vitro upon phosphorylation at Ser-74 by CSNK1D/CK1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the DCK/DGK family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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