POLR2C Antibody - #DF6617

(5)  
Product: POLR2C Antibody
Catalog: DF6617
Description: Rabbit polyclonal antibody to POLR2C
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 31kDa; 31kD(Calculated).
Uniprot: P19387
RRID: AB_2838579

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(90%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
POLR2C Antibody detects endogenous levels of total POLR2C.
RRID:
AB_2838579
Cite Format: Affinity Biosciences Cat# DF6617, RRID:AB_2838579.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA directed RNA polymerase II 33 kDa polypeptide; DNA directed RNA polymerase II subunit C; DNA directed RNA polymerase II subunit RPB3; DNA-directed RNA polymerase II 33 kDa polypeptide; DNA-directed RNA polymerase II subunit C; DNA-directed RNA polymerase II subunit RPB3; hRPB33; hsRPB3; POLR 2C; POLR2 C; Polr2c; Polymerase (RNA) II (DNA directed) polypeptide C, 33kDa; polymerase RNA II DNA directed polypeptide C 33kDa; RNA polymerase II polypeptide C; RNA polymerase II subunit 3; RNA polymerase II subunit B3; RPB 3; RPB3; RPB3_HUMAN; RPB31; RPB33;

Immunogens

Immunogen:
Uniprot:

P19387(RPB3_HUMAN) >>Visit HPA database.

Gene(ID):
POLR2C(5432)
Description:
This gene encodes the third largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a cysteine rich region and exists as a heterodimer with another polymerase subunit, POLR2J. These two subunits form a core subassembly unit of the polymerase. A pseudogene has been identified on chromosome 21.
Sequence:
MPYANQPTVRITELTDENVKFIIENTDLAVANSIRRVFIAEVPIIAIDWVQIDANSSVLHDEFIAHRLGLIPLISDDIVDKLQYSRDCTCEEFCPECSVEFTLDVRCNEDQTRHVTSRDLISNSPRVIPVTSRNRDNDPNDYVEQDDILIVKLRKGQELRLRAYAKKGFGKEHAKWNPTAGVAFEYDPDNALRHTVYPKPEEWPKSEYSELDEDESQAPYDPNGKPERFYYNVESCGSLRPETIVLSALSGLKKKLSDLQTQLSHEIQSDVLTIN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
90
Xenopus
83
Chicken
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P19387 As Substrate

Site PTM Type Enzyme
T12 Phosphorylation
K20 Ubiquitination
K81 Ubiquitination
S124 Phosphorylation
Y142 Phosphorylation
K152 Ubiquitination
K175 Sumoylation
K175 Ubiquitination
K199 Sumoylation
K199 Ubiquitination
K205 Sumoylation
K205 Ubiquitination
S206 Phosphorylation
Y208 Phosphorylation
S209 Phosphorylation
Y220 Phosphorylation
K225 Ubiquitination
S235 Phosphorylation
K253 Ubiquitination
K255 Ubiquitination
S257 Phosphorylation

Research Backgrounds

Function:

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. RPB11/POLR2J and RPB3/POLR2C subunits interact with each other.

Family&Domains:

Belongs to the archaeal RpoD/eukaryotic RPB3 RNA polymerase subunit family.

Research Fields

· Genetic Information Processing > Transcription > RNA polymerase.

· Human Diseases > Neurodegenerative diseases > Huntington's disease.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.