MX1 Antibody - #DF6612

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Product: MX1 Antibody
Catalog: DF6612
Description: Rabbit polyclonal antibody to MX1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 76kDa; 76kD(Calculated).
Uniprot: P20591
RRID: AB_2838574

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
MX1 Antibody detects endogenous levels of total MX1.
RRID:
AB_2838574
Cite Format: Affinity Biosciences Cat# DF6612, RRID:AB_2838574.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Human interferon regulated resistance GTP binding protein MXA; IFI 78; IFI 78K; IFI-78K; IFI78; IFI78K; Interferon induced GTP binding protein Mx1; Interferon induced protein p78; Interferon inducible protein p78; Interferon regulated resistance GTP binding protein; Interferon regulated resistance GTP binding protein MxA; Interferon-induced GTP-binding protein Mx1; Interferon-induced protein p78; Interferon-regulated resistance GTP-binding protein MxA; MX 1; MX; MX dynamin-like GTPase 1; MX1; MX1_HUMAN; MxA; Myxoma resistance protein 1; Myxovirus (influenza virus) resistance 1 interferon inducible; Myxovirus (influenza virus) resistance 1 interferon inducible protein p78; Myxovirus (influenza) resistance 1 homolog of murine; Myxovirus resistance 1, mouse, homolog of; Myxovirus resistance protein 1; N-terminally processed;

Immunogens

Immunogen:
Uniprot:

P20591(MX1_HUMAN) >>Visit HPA database.

Gene(ID):
MX1(4599)
Description:
Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β interferon (INF). This antibody can recognize the endogenous Mx1 protein in several tissues like lung and spleen.
Sequence:
MVVSEVDIAKADPAAASHPLLLNGDATVAQKNPGSVAENNLCSQYEEKVRPCIDLIDSLRALGVEQDLALPAIAVIGDQSSGKSSVLEALSGVALPRGSGIVTRCPLVLKLKKLVNEDKWRGKVSYQDYEIEISDASEVEKEINKAQNAIAGEGMGISHELITLEISSRDVPDLTLIDLPGITRVAVGNQPADIGYKIKTLIKKYIQRQETISLVVVPSNVDIATTEALSMAQEVDPEGDRTIGILTKPDLVDKGTEDKVVDVVRNLVFHLKKGYMIVKCRGQQEIQDQLSLSEALQREKIFFENHPYFRDLLEEGKATVPCLAEKLTSELITHICKSLPLLENQIKETHQRITEELQKYGVDIPEDENEKMFFLIDKVNAFNQDITALMQGEETVGEEDIRLFTRLRHEFHKWSTIIENNFQEGHKILSRKIQKFENQYRGRELPGFVNYRTFETIVKQQIKALEEPAVDMLHTVTDMVRLAFTDVSIKNFEEFFNLHRTAKSKIEDIRAEQEREGEKLIRLHFQMEQIVYCQDQVYRGALQKVREKELEEEKKKKSWDFGAFQSSSATDSSMEEIFQHLMAYHQEASKRISSHIPLIIQFFMLQTYGQQLQKAMLQLLQDKDTYSWLLKERSDTSDKRKFLKERLARLTQARRRLAQFPG

PTMs - P20591 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S17 Phosphorylation
K31 Ubiquitination
S35 Phosphorylation
S58 Phosphorylation
S80 Phosphorylation
K83 Ubiquitination
S99 Phosphorylation
K110 Ubiquitination
K113 Ubiquitination
K119 Ubiquitination
Y126 Phosphorylation
Y129 Phosphorylation
Y196 Phosphorylation
K197 Ubiquitination
K273 Ubiquitination
K279 Ubiquitination
Y308 Phosphorylation
K317 Ubiquitination
K337 Ubiquitination
K359 Ubiquitination
K432 Ubiquitination
K435 Ubiquitination
Y451 Phosphorylation
K459 Ubiquitination
K490 Ubiquitination
K505 Ubiquitination
S558 Phosphorylation
K631 Ubiquitination

Research Backgrounds

Function:

Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium channel activity of TRPCs.

PTMs:

ISGylated.

Subcellular Location:

Cytoplasm. Endoplasmic reticulum membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasm>Perinuclear region.
Note: Binds preferentially to negatively charged phospholipids (PubMed:21900240). Colocalizes with CCHFV protein N in the perinuclear region (PubMed:15047845).

Cytoplasm. Nucleus.
Note: Translocates into the nuclei of HSV-1 infected cells (PubMed:20603636).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer. Oligomerizes into multimeric filamentous or ring-like structures by virtue of its stalk domain. Oligomerization is critical for GTPase activity, protein stability, and recognition of viral target structures. Interacts with TRPC1, TRPC3, TRPC4, TRPC5, TRPC6 and TRPC7. Interacts with HSPA5. Interacts with DDX39A and DDX39B. Interacts with TUBB/TUBB5 (By similarity). The GTP-bound form interacts (via C-terminus) with THOV P5 protein. The GTP-bound form interacts with LACV protein N. Interacts with CCHFV protein N.

Family&Domains:

The C-terminal GTPase effector domain (GED) is involved in oligomerization and viral target recognition.

The middle domain mediates self-assembly and oligomerization.

Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

Restrictive clause

 

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