Product: HMBS Antibody
Catalog: DF6611
Description: Rabbit polyclonal antibody to HMBS
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 39kDa; 39kD(Calculated).
Uniprot: P08397
RRID: AB_2838573

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(91%), Horse(88%), Sheep(91%), Rabbit(91%), Dog(100%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
HMBS Antibody detects endogenous levels of total HMBS.
RRID:
AB_2838573
Cite Format: Affinity Biosciences Cat# DF6611, RRID:AB_2838573.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HEM3_HUMAN; HMBS; Hydroxymethylbilane synthase; PBG D; PBG-D; PBGD; PORC; Porphobilinogen deaminase; porphyria, acute; Chester type; Pre uroporphyrinogen synthase; Pre-uroporphyrinogen synthase; UPS; Uroporphyrinogen I synthase; Uroporphyrinogen I synthetase;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P08397 HEM3_HUMAN:

Isoform 1 is ubiquitously expressed. Isoform 2 is found only in erythroid cells.

Description:
This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Sequence:
MSGNGNAAATAEENSPKMRVIRVGTRKSQLARIQTDSVVATLKASYPGLQFEIIAMSTTGDKILDTALSKIGEKSLFTKELEHALEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTLETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFSAIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWSLDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNLGISLANLLLSKGAKNILDVARQLNDAH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Bovine
91
Sheep
91
Chicken
91
Rabbit
91
Horse
88
Xenopus
82
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08397 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S15 Phosphorylation
K17 Acetylation
S69 Phosphorylation
K70 Ubiquitination
K74 Acetylation
K74 Ubiquitination
K79 Ubiquitination
K87 Acetylation
C114 S-Nitrosylation
K115 Ubiquitination
K128 Ubiquitination
K132 Ubiquitination
K140 Ubiquitination
S147 Phosphorylation
K157 Ubiquitination
K227 Ubiquitination
C261 S-Nitrosylation
S262 Phosphorylation
T269 Phosphorylation
K345 Ubiquitination
K348 Ubiquitination

Research Backgrounds

Function:

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 1 is ubiquitously expressed. Isoform 2 is found only in erythroid cells.

Family&Domains:

Belongs to the HMBS family.

Research Fields

· Metabolism > Metabolism of cofactors and vitamins > Porphyrin and chlorophyll metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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