GGT1 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Myeloma cells, using GGT1 Antibody.

Product:	GGT1 Antibody
Catalog:	DF6610
Description:	Rabbit polyclonal antibody to GGT1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	60kDa; 61kD(Calculated).
Uniprot:	P19440
RRID:	AB_2838572

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

GGT1 Antibody detects endogenous levels of total GGT1.

RRID:

AB_2838572
Cite Format: Affinity Biosciences Cat# DF6610, RRID:AB_2838572.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD224; D22S672; D22S732; Gamma glutamyl transpeptidase; Gamma glutamyltransferase 1; Gamma glutamyltranspeptidase 1; Gamma-glutamyltransferase 1; Gamma-glutamyltranspeptidase 1 light chain; GGT 1; GGT; GGT1; GGT1_HUMAN; Glutamyl transpeptidase; Glutathione hydrolase 1; GTG; Leukotriene C4 hydrolase; MGC96892; MGC96904; MGC96963; OTTHUMP00000028921; OTTHUMP00000197959;

Immunogens

Immunogen:

Uniprot:

P19440(GGT1_HUMAN) >>Visit HPA database.

Gene(ID):

GGT1(2678)

Expression:

P19440 GGT1_HUMAN:

Detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Isoform 3 is lung-specific. There are several other tissue-specific forms that arise from alternative promoter usage but that produce the same protein.

Description:

The enzyme encoded by this gene catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. The enzyme is composed of a heavy chain and a light chain, which are derived from a single precursor protein, and is present in tissues involved in absorption and secretion. This enzyme is a member of the gamma-glutamyltransferase protein family, of which many members have not yet been fully characterized and some of which may represent pseudogenes. This gene is classified as type I gamma-glutamyltransferase. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Oct 2008]

Sequence:

P19440 GGT1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKKKLVVLGLLAVVLVLVIVGLCLWLPSASKEPDNHVYTRAAVAADAKQCSKIGRDALRDGGSAVDAAIAALLCVGLMNAHSMGIGGGLFLTIYNSTTRKAEVINAREVAPRLAFATMFNSSEQSQKGGLSVAVPGEIRGYELAHQRHGRLPWARLFQPSIQLARQGFPVGKGLAAALENKRTVIEQQPVLCEVFCRDRKVLREGERLTLPQLADTYETLAIEGAQAFYNGSLTAQIVKDIQAAGGIVTAEDLNNYRAELIEHPLNISLGDVVLYMPSAPLSGPVLALILNILKGYNFSRESVESPEQKGLTYHRIVEAFRFAYAKRTLLGDPKFVDVTEVVRNMTSEFFAAQLRAQISDDTTHPISYYKPEFYTPDDGGTAHLSVVAEDGSAVSATSTINLYFGSKVRSPVSGILFNNEMDDFSSPSITNEFGVPPSPANFIQPGKQPLSSMCPTIMVGQDGQVRMVVGAAGGTQITTATALAIIYNLWFGYDVKRAVEEPRLHNQLLPNVTTVERNIDQAVTAALETRHHHTQIASTFIAVVQAIVRTAGGWAAASDSRKGGEPAGY

PTMs - P19440 As Substrate

P19440

Site	PTM Type	Source
N95	N-Glycosylation	Uniprot
N120	N-Glycosylation	Uniprot
K172	Ubiquitination	Uniprot
K181	Ubiquitination	Uniprot
N230	N-Glycosylation	Uniprot
N266	N-Glycosylation	Uniprot
N297	N-Glycosylation	Uniprot
K334	Ubiquitination	Uniprot
N344	N-Glycosylation	Uniprot
N511	N-Glycosylation	Uniprot
T550	Phosphorylation	Uniprot

Research Backgrounds

P19440_GGT1_HUMAN

Function:

Cleaves the gamma-glutamyl bond of extracellular glutathione (gamma-Glu-Cys-Gly), glutathione conjugates, and other gamma-glutamyl compounds. The metabolism of glutathione releases free glutamate and the dipeptide cysteinyl-glycine, which is hydrolyzed to cysteine and glycine by dipeptidases. In the presence of high concentrations of dipeptides and some amino acids, can also catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid to form a new gamma-glutamyl compound. Initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracellular GSH level. It is part of the cell antioxidant defense mechanism. Isoform 3 seems to be inactive.

PTMs:

N-glycosylated on both chains. Contains hexoses, hexosamines and sialic acid residues. Glycosylation profiles tested in kidney and liver tissues reveal the presence of tissue-specific and site-specific glycan composition, despite the overlap in composition among the N-glycans. A total of 36 glycan compositions, with 40 unique structures are observed. Up to 15 different glycans are observed at a single site, with site-specific variation in glycan composition. The difference in glycosylation profiles in the 2 tissues do not affect the enzyme activity.

Cleaved by autocatalysis into a large and a small subunit and the autocatalytic cleavage is essential to the functional activation of the enzyme.

Subcellular Location:

Cell membrane>Single-pass type II membrane protein.

Tissue Specificity:

Subunit Structure:

Heterodimer composed of the light and heavy chains. The active site is located in the light chain.

Family&Domains:

Belongs to the gamma-glutamyltransferase family.

Research Fields

· Metabolism > Metabolism of other amino acids > Taurine and hypotaurine metabolism.

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Reconstructing hepatic metabolic profile and glutathione-mediated metabolic fate of acrylamide. Environmental Pollution, 2023 (PubMed: 37673322) [IF=8.9]

2). MicroRNA-142-3p promotes renal cell carcinoma progression by targeting RhoBTB3 to regulate HIF-1 signaling and GGT/GSH pathways. Scientific reports, 2023 (PubMed: 37045834) [IF=4.6]

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GGT1 Antibody - #DF6610