BUB1B Antibody - #DF6609

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Product: BUB1B Antibody
Catalog: DF6609
Description: Rabbit polyclonal antibody to BUB1B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 120kDa; 120kD(Calculated).
Uniprot: O60566
RRID: AB_2838571

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
BUB1B Antibody detects endogenous levels of total BUB1B.
RRID:
AB_2838571
Cite Format: Affinity Biosciences Cat# DF6609, RRID:AB_2838571.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Beta homolg of S. cerevisiae BUB 1; Beta homolg of S. cerevisiae budding uninhibited by benzimidazoles; BUB 1B; BUB1 budding uninhibited by benzimidazoles 1 homolog beta; Bub1A; BUB1B; BUB1B_HUMAN; BUB1beta; BUBR1; Budding Uninhibited by Benzimidazoles 1 beta; Budding uninhibited by benzimidazoles 1 homolog beta (yeast); hBUBR1; MAD3/BUB1 related protein kinase; MAD3/BUB1-related protein kinase; MAD3L; Mitotic checkpoint gene BUB1B; Mitotic checkpoint kinase MAD3L; Mitotic checkpoint serine/threonine protein kinase BUB1 beta; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; MVA1; OTTHUMP00000160319; Protein SSK1; SSK 1; SSK1;

Immunogens

Immunogen:
Uniprot:

O60566(BUB1B_HUMAN) >>Visit HPA database.

Gene(ID):
BUB1B(701)
Expression:
O60566 BUB1B_HUMAN:

Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.

Description:
The Mitotic Checkpoint Complex (MCC), which contains Bub1, Bub1b, Bub3, Mad2, and Cdc20, controls chromosome segregation and monitors kinetochore-microtubule interactions (1). During mitosis, the MCC complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/Cyclosome (APC/C), thereby preventing cells with unaligned chromosomes from prematurely entering anaphase (2). Research studies have shown that Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes, and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6), and is required for functional microtubule-kinetochore interactions (7).
Sequence:
MAAVKKEGGALSEAMSLEGDEWELSKENVQPLRQGRIMSTLQGALAQESACNNTLQQQKRAFEYEIRFYTGNDPLDVWDRYISWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYSDPRFLNLWLKLGRLCNEPLDMYSYLHNQGIGVSLAQFYISWAEEYEARENFRKADAIFQEGIQQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVFESSVPQRSTLAELKSKGKKTARAPIIRVGGALKAPSQNRGLQNPFPQQMQNNSRITVFDENADEASTAELSKPTVQPWIAPPMPRAKENELQAGPWNTGRSLEHRPRGNTASLIAVPAVLPSFTPYVEETARQPVMTPCKIEPSINHILSTRKPGKEEGDPLQRVQSHQQASEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKEQREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERTGDQQEETMPTKETTKLQIASESQKIPGMTLSSSVCQVNCCARETSLAENIWQEQPHSKGPSVPFSIFDEFLLSEKKNKSPPADPPRVLAQRRPLAVLKTSESITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNVTICPNPEDTCDFARAARFVSTPFHEIMSLKDLPSDPERLLPEEDLDVKTSEDQQTACGTIYSQTLSIKKLSPIIEDSREATHSSGFSGSSASVASTSSIKCLQIPEKLELTNETSENPTQSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60566 As Substrate

Site PTM Type Enzyme
S16 Phosphorylation
K26 Ubiquitination
S39 Phosphorylation
T40 Phosphorylation
T54 Phosphorylation
K59 Ubiquitination
S83 Phosphorylation
K113 Ubiquitination
K170 Ubiquitination
K182 Ubiquitination
K210 Ubiquitination
K231 Ubiquitination
S232 Phosphorylation
T237 Phosphorylation
K250 Acetylation
K250 Sumoylation
K250 Ubiquitination
K304 Ubiquitination
T315 Phosphorylation
S318 Phosphorylation
S367 Phosphorylation
T368 Phosphorylation
K370 Ubiquitination
S384 Phosphorylation
Y404 Phosphorylation
T434 Phosphorylation
S435 Phosphorylation P33981 (TTK)
K438 Ubiquitination
K451 Acetylation
K454 Ubiquitination
T471 Phosphorylation
K475 Ubiquitination
K479 Ubiquitination
K488 Ubiquitination
S521 Phosphorylation
K522 Ubiquitination
S537 Phosphorylation
K539 Ubiquitination
K542 Ubiquitination
S543 Phosphorylation P06493 (CDK1) , P33981 (TTK)
S574 Phosphorylation
T600 Phosphorylation
T608 Phosphorylation
T620 Phosphorylation P06493 (CDK1)
K629 Ubiquitination
S633 Phosphorylation
Y660 Phosphorylation
S665 Phosphorylation
K667 Ubiquitination
K668 Ubiquitination
S670 Phosphorylation P33981 (TTK) , P06493 (CDK1)
S676 Phosphorylation P53350 (PLK1)
T680 Phosphorylation P53350 (PLK1)
S683 Phosphorylation
S697 Phosphorylation
K699 Ubiquitination
K706 Ubiquitination
T710 Phosphorylation
S720 Phosphorylation
S733 Phosphorylation
K757 Ubiquitination
Y766 Phosphorylation
K769 Ubiquitination
T792 Phosphorylation P53350 (PLK1)
S797 Phosphorylation
S884 Phosphorylation
K902 Ubiquitination
R919 Methylation
K988 Ubiquitination
K995 Ubiquitination
T1008 Phosphorylation P53350 (PLK1)
K1037 Ubiquitination
K1040 Ubiquitination
T1042 Phosphorylation
S1043 Phosphorylation P06493 (CDK1) , P33981 (TTK)

Research Backgrounds

Function:

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

PTMs:

Proteolytically cleaved by caspase-3 in a cell cycle specific manner. The cleavage might be involved in the durability of the cell cycle delay. Caspase-3 cleavage is associated with abrogation of the mitotic checkpoint. The major site of cleavage is at Asp-610.

Acetylation at Lys-250 regulates its degradation and timing in anaphase entry.

Ubiquitinated. Degraded by the proteasome.

Sumoylated with SUMO2 and SUMO3. The sumoylation mediates the association with CENPE at the kinetochore.

Autophosphorylated in vitro. Intramolecular autophosphorylation is stimulated by CENPE. Phosphorylated during mitosis and hyperphosphorylated in mitotically arrested cells. Phosphorylation at Ser-670 and Ser-1043 occurs at kinetochores upon mitotic entry with dephosphorylation at the onset of anaphase.

Subcellular Location:

Cytoplasm. Nucleus. Chromosome>Centromere>Kinetochore. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Cytoplasmic in interphase cells. Associates with the kinetochores in early prophase. Kinetochore localization requires BUB1, PLK1 and KNL1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index.

Subunit Structure:

Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with KNL1. Interacts with RIPK3.

Family&Domains:

The D-box targets the protein for rapid degradation by ubiquitin-dependent proteolysis during the transition from mitosis to interphase.

The BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

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