IFNAR2 Antibody | Affinity Biosciences

1/1

Western blot analysis of extracts from VERO cells, using IFNAR2 Antibody.

Product:	IFNAR2 Antibody
Catalog:	DF6605
Description:	Rabbit polyclonal antibody to IFNAR2
Application:	WB IHC
Reactivity:	Human, Monkey
Prediction:	Dog
Mol.Wt.:	58kDa,70kDa; 58kD(Calculated).
Uniprot:	P48551
RRID:	AB_2838567

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Monkey

Prediction:

Dog(83%)

Clonality:

Polyclonal

Specificity:

IFNAR2 Antibody detects endogenous levels of total IFNAR2.

RRID:

AB_2838567
Cite Format: Affinity Biosciences Cat# DF6605, RRID:AB_2838567.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Human interferon alpha/beta receptor; IFN alpha REC; IFN R; IFN-a/ßR2; IFN-a/ßRß; IFN-alpha binding protein; IFN-alpha/beta receptor 2; IFN-R-2; IFNABR; IFNAR2; IFNARB; IFNR; INAR2_HUMAN; Interferon (alpha beta and omega) receptor 2; Interferon alpha binding protein; Interferon alpha/beta receptor 2; Interferon alpha/beta receptor beta chain; Type I interferon receptor 2; Type I interferon receptor;

Immunogens

Immunogen:

Uniprot:

P48551(INAR2_HUMAN) >>Visit HPA database.

Gene(ID):

IFNAR2(3455)

Expression:

P48551 INAR2_HUMAN:

Isoform 3 is detected in the urine (at protein level) (PubMed:8181059, PubMed:7759950). Expressed in blood cells. Expressed in lymphoblastoid and fibrosarcoma cell lines.

Description:

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene.

Sequence:

P48551 INAR2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLSQNAFIFRSLNLVLMVYISLVFGISYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESESAESAKIGGIITVFLIALVLTSTIVTLKWIGYICLRNSLPKVLNFHNFLAWPFPNLPPLEAMDMVEVIYINRKKKVWDYNYDDESDSDTEAAPRTSGGGYTMHGLTVRPLGQASATSTESQLIDPESEEEPDLPEVDVELPTMPKDSPQQLELLSGPCERRKSPLQDPFPEEDYSSTEGSGGRITFNVDLNSVFLRVLDDEDSDDLEAPLMLSSHLEEMVDPEDPDNVQSNHLLASGEGTQPTFPSPSSEGLWSEDAPSDQSDTSESDVDLGDGYIMR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Bovine

Sheep

Pig

Horse

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48551 As Substrate

P48551

Site	PTM Type	Source
K80	Ubiquitination	Uniprot
N87	N-Glycosylation	Uniprot
N192	N-Glycosylation	Uniprot
T204	Phosphorylation	Uniprot
T249	Phosphorylation	Uniprot
Y269	Phosphorylation	Uniprot
Y306	Phosphorylation	Uniprot
Y316	Phosphorylation	Uniprot
Y318	Phosphorylation	Uniprot
S322	Phosphorylation	Uniprot
S324	Phosphorylation	Uniprot
T326	Phosphorylation	Uniprot
Y337	Phosphorylation	Uniprot
S364	Phosphorylation	Uniprot
S384	Phosphorylation	Uniprot
K399	Acetylation	Uniprot
S400	Phosphorylation	Uniprot
Y411	Phosphorylation	Uniprot
S412	Phosphorylation	Uniprot
Y512	Phosphorylation	Uniprot

Research Backgrounds

P48551_INAR2_HUMAN

Function:

Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity.

PTMs:

Phosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-337 or Tyr-512 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types.

Glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Cell membrane>Single-pass type I membrane protein.

Secreted.

Tissue Specificity:

Isoform 3 is detected in the urine (at protein level). Expressed in blood cells. Expressed in lymphoblastoid and fibrosarcoma cell lines.

Subunit Structure:

Heterodimer with IFNAR1. Isoform 1 interacts with the transcriptional factors STAT1 and STAT2. Interacts with JAK1.

Family&Domains:

Belongs to the type II cytokine receptor family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Influenza A.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

· Organismal Systems > Immune system > Toll-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

IFNAR2 Antibody - #DF6605