Ferritin Light Chain Antibody - #DF6604

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Product: Ferritin Light Chain Antibody
Catalog: DF6604
Description: Rabbit polyclonal antibody to Ferritin Light Chain
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Xenopus
Mol.Wt.: 19kDa; 20kD(Calculated).
Uniprot: P02792
RRID: AB_2838566

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Xenopus(80%)
Clonality:
Polyclonal
Specificity:
Ferritin Light Chain Antibody detects endogenous levels of total Ferritin Light Chain.
RRID:
AB_2838566
Cite Format: Affinity Biosciences Cat# DF6604, RRID:AB_2838566.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ferritin L chain; Ferritin L subunit; Ferritin light chain; Ferritin light polypeptide; ferritin light polypeptide like 3; FRIL_HUMAN; FTL; LFTD; NBIA 3; NBIA3;

Immunogens

Immunogen:
Uniprot:

P02792(FRIL_HUMAN) >>Visit HPA database.

Gene(ID):
FTL(2512)
Description:
This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.
Sequence:
MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Xenopus
80
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02792 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S3 Phosphorylation
K59 Ubiquitination
K68 Ubiquitination
K83 Ubiquitination
K84 Ubiquitination
K92 Ubiquitination
K98 Acetylation
K98 Ubiquitination
K105 Ubiquitination
K106 Ubiquitination
S119 Phosphorylation
C127 S-Nitrosylation
K140 Ubiquitination
K144 Ubiquitination
Y165 Phosphorylation

Research Backgrounds

Function:

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity).

Subunit Structure:

Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Iron enters the spherical protein shell through pores that are formed between subunits. Mutations leading to truncation or the addition of extra residues at the C-terminus interfere with normal pore formation and with iron accumulation.

Family&Domains:

Belongs to the ferritin family.

Research Fields

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Organismal Systems > Digestive system > Mineral absorption.

References

1). Alleviated NCOA4-mediated ferritinophagy protected RA FLSs from ferroptosis in lipopolysaccharide-induced inflammation under hypoxia. Inflammation research : official journal of the European Histamine Research Society ... [et al.], 2024 (PubMed: 38189810) [IF=6.7]
2). Zinc oxide nanoparticles induces cell death and consequently leading to incomplete neural tube closure through oxidative stress during embryogenesis. Cell biology and toxicology, 2024 (PubMed: 38958792) [IF=6.1]
3). NCOA4‐mediated ferritinophagy promoted inflammatory responses in periodontitis. JOURNAL OF PERIODONTAL RESEARCH, 2021 (PubMed: 33533512) [IF=3.5]

Application: IHC    Species: Human    Sample: gingival tissues

FIGURE 1 Increased expression of iron metabolism-related genes in gingival tissues. Samples from normal control (NC) (n = 8) and chronic periodontitis patients (CP) (n = 12) were stained for NCOA4 (A&B), FTH1 (C&D), and FTL (E&F). The scale bar represents 100 µm. Figures (a1/2–f1/2) showed higher magnifications of the same sections. The scale bar represented 20 µm. (G) Semi-quantitative results (mean ±SD) of immunostaining for NCOA4, FTH1, and FTL in the normal control and periodontitis group. *p < .05; **p < .01
4). Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells. Oxidative medicine and cellular longevity, 2021 (PubMed: 34707773)

Application: WB    Species: Human    Sample: OS cells

Figure 2 Bavachin-induced ferrous iron accumulation initiates death of osteosarcoma (OS) cells. (a) Bavachin (24 h) induced ferrous iron accumulation in OS cells occurs in a concentration-dependent manner. (b) Deferoxamine (DFO) rescues cells from bavachin-induced cell death. (c, d) Western blotting analysis shows the protein levels of transferrin receptor (TFRC), divalent metal transporter-1 (DMT1), ferritin light chain (FTH), and ferritin heavy chain (FTL) in MG63 and HOS cells treated with bavachin (10 μM, 20 μM, and 40 μM) for 24 h. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and #p < 0.001 vs. the control group.

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