IL16 Antibody - #DF6600

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Product: IL16 Antibody
Catalog: DF6600
Description: Rabbit polyclonal antibody to IL16
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep
Mol.Wt.: 142kDa; 142kD(Calculated).
Uniprot: Q14005
RRID: AB_2838562

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Horse(100%), Sheep(100%)
Clonality:
Polyclonal
Specificity:
IL16 Antibody detects endogenous levels of total IL16.
RRID:
AB_2838562
Cite Format: Affinity Biosciences Cat# DF6600, RRID:AB_2838562.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HGNC:5980; HsT19289; IL-16; IL16; IL16_HUMAN; Interleukin 16 precursor; Interleukin-16; LCF; Lymphocyte chemoattractant factor; Neuronal interleukin 16; NIL16; prIL 16; PrIL16; Prointerleukin 16;

Immunogens

Immunogen:
Uniprot:

Q14005(IL16_HUMAN) >>Visit HPA database.

Gene(ID):
IL16(3603)
Expression:
Q14005 IL16_HUMAN:

Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.

Description:
The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 is reported to be involved in the proteolytic processing of this protein. Alternate splicing results in multiple transcript variants.
Sequence:
MESHSRAGKSRKSAKFRSISRSLMLCNAKTSDDGSSPDEKYPDPFEISLAQGKEGIFHSSVQLADTSEAGPSSVPDLALASEAAQLQAAGNDRGKTCRRIFFMKESSTASSREKPGKLEAQSSNFLFPKACHQRARSNSTSVNPYCTREIDFPMTKKSAAPTDRQPYSLCSNRKSLSQQLDCPAGKAAGTSRPTRSLSTAQLVQPSGGLQASVISNIVLMKGQAKGLGFSIVGGKDSIYGPIGIYVKTIFAGGAAAADGRLQEGDEILELNGESMAGLTHQDALQKFKQAKKGLLTLTVRTRLTAPPSLCSHLSPPLCRSLSSSTCITKDSSSFALESPSAPISTAKPNYRIMVEVSLQKEAGVGLGIGLCSVPYFQCISGIFVHTLSPGSVAHLDGRLRCGDEIVEISDSPVHCLTLNEVYTILSHCDPGPVPIIVSRHPDPQVSEQQLKEAVAQAVENTKFGKERHQWSLEGVKRLESSWHGRPTLEKEREKNSAPPHRRAQKVMIRSSSDSSYMSGSPGGSPGSGSAEKPSSDVDISTHSPSLPLAREPVVLSIASSRLPQESPPLPESRDSHPPLRLKKSFEILVRKPMSSKPKPPPRKYFKSDSDPQKSLEERENSSCSSGHTPPTCGQEARELLPLLLPQEDTAGRSPSASAGCPGPGIGPQTKSSTEGEPGWRRASPVTQTSPIKHPLLKRQARMDYSFDTTAEDPWVRISDCIKNLFSPIMSENHGHMPLQPNASLNEEEGTQGHPDGTPPKLDTANGTPKVYKSADSSTVKKGPPVAPKPAWFRQSLKGLRNRASDPRGLPDPALSTQPAPASREHLGSHIRASSSSSSIRQRISSFETFGSSQLPDKGAQRLSLQPSSGEAAKPLGKHEEGRFSGLLGRGAAPTLVPQQPEQVLSSGSPAASEARDPGVSESPPPGRQPNQKTLPPGPDPLLRLLSTQAEESQGPVLKMPSQRARSFPLTRSQSCETKLLDEKTSKLYSISSQVSSAVMKSLLCLPSSISCAQTPCIPKEGASPTSSSNEDSAANGSAETSALDTGFSLNLSELREYTEGLTEAKEDDDGDHSSLQSGQSVISLLSSEELKKLIEEVKVLDEATLKQLDGIHVTILHKEEGAGLGFSLAGGADLENKVITVHRVFPNGLASQEGTIQKGNEVLSINGKSLKGTTHHDALAILRQAREPRQAVIVTRKLTPEAMPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Xenopus
75
Zebrafish
75
Rabbit
75
Dog
57
Pig
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14005 As Substrate

Site PTM Type Enzyme
S139 Phosphorylation
S175 Phosphorylation
S177 Phosphorylation
T298 Phosphorylation
T301 Phosphorylation
S322 Phosphorylation
T325 Phosphorylation
S331 Phosphorylation
S471 Phosphorylation
S480 Phosphorylation
S481 Phosphorylation
S566 Phosphorylation
S572 Phosphorylation
S584 Phosphorylation
S614 Phosphorylation
T631 Phosphorylation
S683 Phosphorylation
S705 Phosphorylation
S743 Phosphorylation P68400 (CSNK2A1)
T757 Phosphorylation P06493 (CDK1)
Y771 Phosphorylation
K772 Ubiquitination
K788 Ubiquitination
S795 Phosphorylation
S804 Phosphorylation
S833 Phosphorylation
S834 Phosphorylation
S835 Phosphorylation
S836 Phosphorylation
S844 Phosphorylation
S845 Phosphorylation P28482 (MAPK1) , P27361 (MAPK3)
T848 Phosphorylation
S851 Phosphorylation
S852 Phosphorylation
K857 Ubiquitination
S863 Phosphorylation
R882 Methylation
S884 Phosphorylation
R889 Methylation
S920 Phosphorylation
S922 Phosphorylation
T933 Phosphorylation
S946 Phosphorylation
T947 Phosphorylation
S952 Phosphorylation
S961 Phosphorylation
S966 Phosphorylation
T970 Phosphorylation
S972 Phosphorylation
S974 Phosphorylation
K978 Methylation
S989 Phosphorylation
S995 Phosphorylation
S996 Phosphorylation
S1001 Phosphorylation
S1074 Phosphorylation
S1247 Phosphorylation
S1254 Phosphorylation
K1259 Ubiquitination
K1305 Ubiquitination
S1320 Phosphorylation
K1324 Ubiquitination

Research Backgrounds

Function:

Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.

Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.

Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells.

PTMs:

Isoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16.

Subcellular Location:

Secreted.

Cytoplasm.

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T-cell proliferation.

Subunit Structure:

Homotetramer (Probable). According to the formation of a homotetrameric protein complex is not required for the chemo-attractant function. Isoform 3 interacts (via PDZ 3 domain) with PPP1R12A, PPP1R12B and PPP1R12C. Isoform 1 interacts with PPP1R12B. Isoform 3 interacts with GRIN2A. Isoform 3 interacts with GABPB1. Isoform 3 interacts (via PDZ 3 domain) with HDAC3. Isoform 1 interacts with GRIN2D, KCNJ10, KCNJ15 and CACNA1C (By similarity).

(Microbial infection) Isoform 3 interacts with HTLV-1 tax.

References

1). p.P476S mutation of RBPJL inhibits the efficacy of anti-PD-1 therapy in oesophageal squamous cell carcinoma by blunting T-cell responses. Clinical & Translational Immunology, 2020 (PubMed: 32994998) [IF=5.8]

Application: IHC    Species: Human    Sample:

Figure 5 CM of RBPJL knockdown cells inhibited the chemotaxis and proliferation of T cells and Th1/Th2 differentiation induced by CM from RBPJL‐overexpressing cells. (a) Representative images of RBPJL and IL‐16 staining in responsive oesophagus and lung lesions as well as liver lesions. Scale bars represent 200 μm. The number of positive cells from 3 to 5 fields was counted. The average positive ratio from each section was identified. (b) The chemotaxis of CD4+ and CD8+ T cells as analysed by transwell assay. (c) Effects of CMs from KYSE150 cells on the proliferation of T cells were assessed by loss of CFSE fluorescence. (d) Sorted naïve CD4+ T cells were stimulated by anti‐CD3/CD28 and human IL‐2, and subsequently incubated in the presence of CMs for 5 days, followed by staining with CD4, T‐bet and GATA3 antibodies for flow cytometry analysis. Percentages of CD4+ T‐bet+ and CD4+ GATA3+ T cells are shown. Data are presented as the mean ± standard deviation (n = 3), *P < 0.05, **P < 0.01, ***P < 0.001. The data represent three independent technical replicates.

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