MTTP Antibody | Affinity Biosciences

Cites

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FIGURE 7 | PA treatment attenuated HFD-induced MTP reduction in rats.

Figure 5: |CADE regulates lipid synthesis, decomposition and transport through miR-378b in C57BL/6 mice.

Product:	MTTP Antibody
Catalog:	DF6591
Description:	Rabbit polyclonal antibody to MTTP
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	99kDa; 99kD(Calculated).
Uniprot:	P55157
RRID:	AB_2838553

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

MTTP Antibody detects endogenous levels of total MTTP.

RRID:

AB_2838553
Cite Format: Affinity Biosciences Cat# DF6591, RRID:AB_2838553.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ABL; Microsomal triglyceride transfer protein (large polypeptide 88kDa); Microsomal triglyceride transfer protein 88kD; Microsomal triglyceride transfer protein; Microsomal triglyceride transfer protein large subunit; MTP; MTP large subunit; MTP_HUMAN; MTTP; Transfer RNA, mitochondrial, proline; tRNA-Pro, mitochondrial;

Immunogens

Immunogen:

Uniprot:

P55157(MTP_HUMAN) >>Visit HPA database.

Gene(ID):

MTTP(4547)

Expression:

P55157 MTP_HUMAN:

Liver and small intestine. Also found in ovary, testis and kidney.

Description:

MTP encodes the large subunit of the heterodimeric microsomal triglyceride transfer protein. Protein disulfide isomerase (PDI) completes the heterodimeric microsomal triglyceride transfer protein, which has been shown to play a central role in lipoprotein assembly. Mutations in MTP can cause abetalipoproteinemia. [provided by RefSeq, Jul 2008]

Sequence:

P55157 MTP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MILLAVLFLCFISSYSASVKGHTTGLSLNNDRLYKLTYSTEVLLDRGKGKLQDSVGYRISSNVDVALLWRNPDGDDDQLIQITMKDVNVENVNQQRGEKSIFKGKSPSKIMGKENLEALQRPTLLHLIHGKVKEFYSYQNEAVAIENIKRGLASLFQTQLSSGTTNEVDISGNCKVTYQAHQDKVIKIKALDSCKIARSGFTTPNQVLGVSSKATSVTTYKIEDSFVIAVLAEETHNFGLNFLQTIKGKIVSKQKLELKTTEAGPRLMSGKQAAAIIKAVDSKYTAIPIVGQVFQSHCKGCPSLSELWRSTRKYLQPDNLSKAEAVRNFLAFIQHLRTAKKEEILQILKMENKEVLPQLVDAVTSAQTSDSLEAILDFLDFKSDSSIILQERFLYACGFASHPNEELLRALISKFKGSIGSSDIRETVMIITGTLVRKLCQNEGCKLKAVVEAKKLILGGLEKAEKKEDTRMYLLALKNALLPEGIPSLLKYAEAGEGPISHLATTALQRYDLPFITDEVKKTLNRIYHQNRKVHEKTVRTAAAAIILNNNPSYMDVKNILLSIGELPQEMNKYMLAIVQDILRFEMPASKIVRRVLKEMVAHNYDRFSRSGSSSAYTGYIERSPRSASTYSLDILYSGSGILRRSNLNIFQYIGKAGLHGSQVVIEAQGLEALIAATPDEGEENLDSYAGMSAILFDVQLRPVTFFNGYSDLMSKMLSASGDPISVVKGLILLIDHSQELQLQSGLKANIEVQGGLAIDISGAMEFSLWYRESKTRVKNRVTVVITTDITVDSSFVKAGLETSTETEAGLEFISTVQFSQYPFLVCMQMDKDEAPFRQFEKKYERLSTGRGYVSQKRKESVLAGCEFPLHQENSEMCKVVFAPQPDSTSSGWF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P55157 As Substrate

P55157

Site	PTM Type	Source
K99	Acetylation	Uniprot
K105	Acetylation	Uniprot
S211	Phosphorylation	Uniprot
T261	Phosphorylation	Uniprot
T427	Phosphorylation	Uniprot
T432	Phosphorylation	Uniprot
T434	Phosphorylation	Uniprot
Y511	Phosphorylation	Uniprot
S563	Phosphorylation	Uniprot

Research Backgrounds

P55157_MTP_HUMAN

Function:

Catalyzes the transport of triglyceride, cholesteryl ester, and phospholipid between phospholipid surfaces. Required for the secretion of plasma lipoproteins that contain apolipoprotein B.

Subcellular Location:

Endoplasmic reticulum.
Note: Colocalizes with P4HB/PDI in the endoplasmic reticulum (PubMed:23475612, PubMed:26224785).

Tissue Specificity:

Liver and small intestine. Also found in ovary, testis and kidney.

Subunit Structure:

Heterodimer; heterodimerizes with the protein disulfide isomerase (P4HB/PDI). Interacts with APOB.

Research Fields

· Organismal Systems > Digestive system > Fat digestion and absorption.

References

1). Protective Effect of Patchouli Alcohol Against High-Fat Diet Induced Hepatic Steatosis by Alleviating Endoplasmic Reticulum Stress and Regulating VLDL Metabolism in Rats. Frontiers in Pharmacology, 2019 (PubMed: 31632274) [IF=5.6]

Application: WB Species: rat Sample:

FIGURE 7 | PA treatment attenuated HFD-induced MTP reduction in rats. (A) Representative immunoreactive bands of XBP1, PDI, and MTP

2). Caffeic acid dimethyl ether alleviates alcohol-induced hepatic steatosis via microRNA-378b-mediated CaMKK2-AMPK pathway. Bioengineered, 2022 (PubMed: 35481488) [IF=4.9]

Application: WB Species: mouse Sample: livers

Figure 5: |CADE regulates lipid synthesis, decomposition and transport through miR-378b in C57BL/6 mice. (a,b) Western blot analysis of the protein expressions of SREBP-1c, FASN, PPARα, CPT1, MTTP and protein ratio of p-foxO1/foxO1 when the mice were injected with AAV-miR-378b agomir and antagomir. All data are expressed as the means ± SD of at least three separate experiments.

3). Androgens exacerbate hepatic triglyceride accumulation in rats with polycystic ovary syndrome by downregulating MTTP expression. Endocrine, 2023 (PubMed: 37950821) [IF=3.7]

Restrictive clause

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MTTP Antibody - #DF6591