PTHR1 Antibody - #DF6589

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Product: PTHR1 Antibody
Catalog: DF6589
Description: Rabbit polyclonal antibody to PTHR1
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 66kDa; 66kD(Calculated).
Uniprot: Q03431
RRID: AB_2838551

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
PTHR1 Antibody detects endogenous levels of total PTHR1.
RRID:
AB_2838551
Cite Format: Affinity Biosciences Cat# DF6589, RRID:AB_2838551.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MGC138426; MGC138452; Parathyroid hormone 1 receptor; Parathyroid hormone/parathyroid hormone related peptide receptor; Parathyroid hormone/parathyroid hormone related protein receptor; Parathyroid hormone/parathyroid hormone-related peptide receptor; PTH receptor; PTH/PTHr receptor; PTH/PTHrP receptor; PTH/PTHrP type I receptor; PTH1 receptor; PTH1R; PTH1R_HUMAN; PTHR 1; PTHR; PTHR1;

Immunogens

Immunogen:
Uniprot:

Q03431(PTH1R_HUMAN) >>Visit HPA database.

Gene(ID):
PTH1R(5745)
Expression:
Q03431 PTH1R_HUMAN:

Expressed in most tissues. Most abundant in kidney, bone and liver.

Description:
The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphysealchondrodysplasia (JMC), chondrodysplasiaBlomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]
Sequence:
MGTARIAPGLALLLCCPVLSSAYALVDADDVMTKEEQIFLLHRAQAQCEKRLKEVLQRPASIMESDKGWTSASTSGKPRKDKASGKLYPESEEDKEAPTGSRYRGRPCLPEWDHILCWPLGAPGEVVAVPCPDYIYDFNHKGHAYRRCDRNGSWELVPGHNRTWANYSECVKFLTNETREREVFDRLGMIYTVGYSVSLASLTVAVLILAYFRRLHCTRNYIHMHLFLSFMLRAVSIFVKDAVLYSGATLDEAERLTEEELRAIAQAPPPPATAAAGYAGCRVAVTFFLYFLATNYYWILVEGLYLHSLIFMAFFSEKKYLWGFTVFGWGLPAVFVAVWVSVRATLANTGCWDLSSGNKKWIIQVPILASIVLNFILFINIVRVLATKLRETNAGRCDTRQQYRKLLKSTLVLMPLFGVHYIVFMATPYTEVSGTLWQVQMHYEMLFNSFQGFFVAIIYCFCNGEVQAEIKKSWSRWTLALDFKRKARSGSSSYSYGPMVSHTSVTNVGPRVGLGLPLSPRLLPTATTNGHPQLPGHAKPGTPALETLETTPPAMAAPKDDGFLNGSCSGLDEEASGPERPPALLQEEWETVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
88
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03431 As Substrate

Site PTM Type Enzyme
Y23 Phosphorylation
S73 Phosphorylation
S91 Phosphorylation
T99 Phosphorylation
S101 Phosphorylation
T192 Phosphorylation
Y320 Phosphorylation
T387 Phosphorylation
K388 Ubiquitination
T392 Phosphorylation
S473 Phosphorylation
K484 Ubiquitination
S489 Phosphorylation
S491 Phosphorylation
S492 Phosphorylation
S493 Phosphorylation
Y494 Phosphorylation
S495 Phosphorylation
Y496 Phosphorylation
S501 Phosphorylation
T503 Phosphorylation
S504 Phosphorylation
S519 Phosphorylation
T547 Phosphorylation
T551 Phosphorylation
T591 Phosphorylation

Research Backgrounds

Function:

Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in most tissues. Most abundant in kidney, bone and liver.

Subunit Structure:

Interacts (via N-terminal extracellular domain) with PTHLH and PTH. Homodimer in the absence of bound ligand. Peptide hormone binding leads to dissociation of the homodimer.

Family&Domains:

Belongs to the G-protein coupled receptor 2 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Excretory system > Endocrine and other factor-regulated calcium reabsorption.

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