DNA Ligase IV Antibody | Affinity Biosciences

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Western blot analysis of extracts from Hela, using LIG4 Antibody.

DF6588 at 1/100 staining Mouse heart tissue by IHC-P.

Product:	DNA Ligase IV Antibody
Catalog:	DF6588
Description:	Rabbit polyclonal antibody to DNA Ligase IV
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	104kDa; 104kD(Calculated).
Uniprot:	P49917
RRID:	AB_2838550

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(82%), Zebrafish(82%), Bovine(82%), Horse(100%), Rabbit(91%), Dog(100%), Chicken(100%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

DNA Ligase IV Antibody detects endogenous levels of total DNA Ligase IV.

RRID:

AB_2838550
Cite Format: Affinity Biosciences Cat# DF6588, RRID:AB_2838550.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DNA ligase IV ATP dependent; DNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; DNLI4_HUMAN; LIG 4; LIG4; LIG4S; Ligase IV; Ligase IV DNA ATP dependent; Polydeoxyribonucleotide synthase [ATP] 4; Polydeoxyribonucleotide synthase 4; Polydeoxyribonucleotide synthase; Polynucleotide ligase; Sealase;

Immunogens

Immunogen:

Uniprot:

P49917(DNLI4_HUMAN) >>Visit HPA database.

Gene(ID):

LIG4(3981)

Expression:

P49917 DNLI4_HUMAN:

Testis, thymus, prostate and heart.

Description:

Two major pathways, homologous recombination (HR) and nonhomologous end joining (NHEJ), counteract one of themost toxic lesions, the DSB. The core protein complex mediating NHEJ in mammals includes DNA ligase IV (Lig4). Lig4 belongs to an ATP-dependent DNA ligase family, and joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. The complex Lig4-XRCC4 is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of Lig4.

Sequence:

P49917 DNLI4_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAASQTSQTVASHVPFADLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILPQLERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQKGSLTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDISITLFSAFKPMLAAIADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDLQTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNEAIDKREEGIMVKQPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCAVAEKPPPGEKPSVFHTLSRVGSGCTMKELYDLGLKLAKYWKPFHRKAPPSSILCGTEKPEVYIEPCNSVIVQIKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRGKASGKLASKHLYIGGDDEPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQPKPDLENRIAEFGGYIVQNPGPDTYCVIAGSENIRVKNIILSNKHDVVKPAWLLECFKTKSFVPWQPRFMIHMCPSTKEHFAREYDCYGDSYFIDTDLNQLKEVFSGIKNSNEQTPEEMASLIADLEYRYSWDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVSCLAEGVSHVIIGEDHSRVADFKAFRRTFKRKFKILKESWVTDSIDKCELQEENQYLI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Chicken

100

Xenopus

Rabbit

Pig

Bovine

Zebrafish

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49917 As Substrate

P49917

Site	PTM Type	Enzyme	Source
R40	Methylation		Uniprot
R47	Methylation		Uniprot
K55	Ubiquitination		Uniprot
K85	Ubiquitination		Uniprot
K169	Ubiquitination		Uniprot
S199	Phosphorylation	Q13535 (ATR) , Q13315 (ATM)	Uniprot
S267	Phosphorylation		Uniprot
K273	Ubiquitination		Uniprot
K283	Ubiquitination		Uniprot
Y288	Phosphorylation		Uniprot
K289	Ubiquitination		Uniprot
T347	Phosphorylation		Uniprot
S358	Phosphorylation		Uniprot
T362	Phosphorylation		Uniprot
K376	Ubiquitination		Uniprot
K411	Ubiquitination		Uniprot
K424	Ubiquitination		Uniprot
K432	Methylation		Uniprot
K432	Ubiquitination		Uniprot
K439	Ubiquitination		Uniprot
K442	Methylation		Uniprot
Y454	Phosphorylation		Uniprot
K513	Ubiquitination		Uniprot
K521	Ubiquitination		Uniprot
K571	Ubiquitination		Uniprot
K588	Ubiquitination		Uniprot
K613	Ubiquitination		Uniprot
K645	Ubiquitination		Uniprot
T650	Phosphorylation	P78527 (PRKDC)	Uniprot
S668	Phosphorylation	P78527 (PRKDC)	Uniprot
S672	Phosphorylation	P78527 (PRKDC)	Uniprot
K710	Ubiquitination		Uniprot
K722	Ubiquitination		Uniprot
K733	Ubiquitination		Uniprot
K775	Ubiquitination		Uniprot
K841	Ubiquitination		Uniprot
K876	Ubiquitination		Uniprot
K890	Ubiquitination		Uniprot

Research Backgrounds

P49917_DNLI4_HUMAN

Function:

Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.

Subcellular Location:

Nucleus.

Tissue Specificity:

Testis, thymus, prostate and heart.

Subunit Structure:

Interacts with XRCC4. The LIG4-XRCC4 complex has probably a 1:2 stoichiometry. The LIG4-XRCC4 complex associates in a DNA-dependent manner with the DNA-PK complex composed of PRKDC, XRCC6/Ku70 and XRCC5/Ku86 to form the core non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. Interacts with APLF.

Family&Domains:

Belongs to the ATP-dependent DNA ligase family.

Research Fields

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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DNA Ligase IV Antibody - #DF6588