Annexin V Antibody | Affinity Biosciences

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Annexin V Antibody - Western blot analysis of extracts from mouse brain, using ANXA5 Antibody.

Annexin V Antibody - Western blot analysis of extracts from Hela cells(uv treatment), using Annexin V Antibody.

Product:	Annexin V Antibody
Catalog:	DF6580
Description:	Rabbit polyclonal antibody to Annexin V
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	36kDa; 36kD(Calculated).
Uniprot:	P08758
RRID:	AB_2838542

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(92%), Sheep(92%), Rabbit(92%), Dog(100%), Chicken(90%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

Annexin V Antibody detects endogenous levels of total Annexin V.

RRID:

AB_2838542
Cite Format: Affinity Biosciences Cat# DF6580, RRID:AB_2838542.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Anchorin CII; Annexin 5; Annexin A5; Annexin V; Annexin-5; ANX A5; ANX5; ANXA5; ANXA5_HUMAN; Calphobindin I; CBP-I; Endonexin II; ENX2; Lipocortin V; PAP I; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PLACENTAL PROTEIN 4; Pp4; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha;

Immunogens

Immunogen:

Uniprot:

P08758(ANXA5_HUMAN) >>Visit HPA database.

Gene(ID):

ANXA5(308)

Description:

Annexin V, also known as PAP-1 or Lipocortin V, is a ~30 kDa protein that binds to phospholipids in a calcium-dependent manner (1). All annexins contain a putative PKC binding site, but only annexin V has been identified as an inhibitor of this pathway (2). It may also signal, by direct interaction with VEGFR2 receptor, in the regulation of vascular endothelial cell proliferation (3). Annexin V preferentially binds phosphatidylserine, in competition with prothrombin, leading to inhibition of blood coagulation at sites of injury preceding contact between lipid components and coagulation factors that initiate thrombosis (4-6). The ability of Annexin V to bind specifically and robustly to hosphatidylserine makes it an attractive reagent in detecting apoptotic cells (7). Annexin V is inducible by glucocorticoids and can be phosphorylated by tyrosine and serine/threonine kinases (8). It isthought to blockproduction of mediators of inflammation, such as prostaglandins and leukotrienes by inhibiting the release of arachidonic acid from membranes by phospholipase A2 (8).

Sequence:

P08758 ANXA5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAQVLRGTVTDFPGFDERADAETLRKAMKGLGTDEESILTLLTSRSNAQRQEISAAFKTLFGRDLLDDLKSELTGKFEKLIVALMKPSRLYDAYELKHALKGAGTNEKVLTEIIASRTPEELRAIKQVYEEEYGSSLEDDVVGDTSGYYQRMLVVLLQANRDPDAGIDEAQVEQDAQALFQAGELKWGTDEEKFITIFGTRSVSHLRKVFDKYMTISGFQIEETIDRETSGNLEQLLLAVVKSIRSIPAYLAETLYYAMKGAGTDDHTLIRVMVSRSEIDLFNIRKEFRKNFATSLYSMIKGDTSGDYKKALLLLCGEDD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Dog

100

Horse

Sheep

Rabbit

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08758 As Substrate

P08758

Site	PTM Type	Source
A2	Acetylation	Uniprot
R6	Methylation	Uniprot
T10	Phosphorylation	Uniprot
R18	Methylation	Uniprot
T23	Phosphorylation	Uniprot
K26	Ubiquitination	Uniprot
K29	Sumoylation	Uniprot
K29	Ubiquitination	Uniprot
T33	Phosphorylation	Uniprot
S37	Phosphorylation	Uniprot
T40	Phosphorylation	Uniprot
T43	Phosphorylation	Uniprot
S44	Phosphorylation	Uniprot
K58	Ubiquitination	Uniprot
K70	Acetylation	Uniprot
K70	Sumoylation	Uniprot
K70	Ubiquitination	Uniprot
T74	Phosphorylation	Uniprot
K76	Acetylation	Uniprot
K76	Ubiquitination	Uniprot
K79	Acetylation	Uniprot
K79	Ubiquitination	Uniprot
K86	Acetylation	Uniprot
K86	Ubiquitination	Uniprot
S88	Phosphorylation	Uniprot
Y91	Phosphorylation	Uniprot
Y94	Phosphorylation	Uniprot
K97	Acetylation	Uniprot
K97	Ubiquitination	Uniprot
K101	Acetylation	Uniprot
K101	Ubiquitination	Uniprot
T105	Phosphorylation	Uniprot
K108	Acetylation	Uniprot
K108	Ubiquitination	Uniprot
T111	Phosphorylation	Uniprot
S116	Phosphorylation	Uniprot
T118	Phosphorylation	Uniprot
K126	Sumoylation	Uniprot
K126	Ubiquitination	Uniprot
Y129	Phosphorylation	Uniprot
Y133	Phosphorylation	Uniprot
S135	Phosphorylation	Uniprot
S136	Phosphorylation	Uniprot
K186	Ubiquitination	Uniprot
K193	Ubiquitination	Uniprot
T196	Phosphorylation	Uniprot
K242	Ubiquitination	Uniprot
S246	Phosphorylation	Uniprot
T254	Phosphorylation	Uniprot
Y256	Phosphorylation	Uniprot
Y257	Phosphorylation	Uniprot
K260	Ubiquitination	Uniprot
T264	Phosphorylation	Uniprot
T268	Phosphorylation	Uniprot
S275	Phosphorylation	Uniprot
S277	Phosphorylation	Uniprot
K290	Ubiquitination	Uniprot
S295	Phosphorylation	Uniprot
Y297	Phosphorylation	Uniprot
S298	Phosphorylation	Uniprot
K301	Ubiquitination	Uniprot
K309	Acetylation	Uniprot
K310	Ubiquitination	Uniprot

Research Backgrounds

P08758_ANXA5_HUMAN

Function:

This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.

PTMs:

S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

Subunit Structure:

Monomer. Binds ATRX and EIF5B (By similarity). Interacts with hepatitis B virus (HBV).

Family&Domains:

The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Belongs to the annexin family.

References

1). 膜联蛋白A5在胰腺癌中的预后意义及机制的生物信息学分析. 中国普通外科杂志, 2022

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Annexin V Antibody - #DF6580