PGF Antibody - #DF6579

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Product: PGF Antibody
Catalog: DF6579
Description: Rabbit polyclonal antibody to PGF
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit
Mol.Wt.: 24kDa; 25kD(Calculated).
Uniprot: P49763
RRID: AB_2838541

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Rabbit(88%)
Clonality:
Polyclonal
Specificity:
PGF Antibody detects endogenous levels of total PGF.
RRID:
AB_2838541
Cite Format: Affinity Biosciences Cat# DF6579, RRID:AB_2838541.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

PGF; PGFL; Placenta growth factor; PlGF;

Immunogens

Immunogen:
Uniprot:

P49763(PLGF_HUMAN) >>Visit HPA database.

Gene(ID):
PGF(5228)
Expression:
P49763 PLGF_HUMAN:

While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.

Description:
This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.
Sequence:
MPVMRLFPCFLQLLAGLALPAVPPQQWALSAGNGSSEVEVVPFQEVWGRSYCRALERLVDVVSEYPSEVEHMFSPSCVSLLRCTGCCGDENLHCVPVETANVTMQLLKIRSGDRPSYVELTFSQHVRCECRHSPGRQSPDMPGDFRADAPSFLPPRRSLPMLFRMEWGCALTGSQSAVWPSSPVPEEIPRMHPGRNGKKQQRKPLREKMKPERCGDAVPRR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Rabbit
88
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49763 As Substrate

Site PTM Type Enzyme
S116 Phosphorylation
Y117 Phosphorylation

Research Backgrounds

Function:

Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Also promotes cell tumor growth.

PTMs:

N-glycosylated.

Subcellular Location:

Secreted.
Note: The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.

Subunit Structure:

Antiparallel homodimer; disulfide-linked. Also found as heterodimer with VEGFA/VEGF. Isoform PlGF-3 is found both as homodimer and as monomer.

Family&Domains:

Isoform PlGF-2 contains a basic insert which acts as a cell retention signal.

Belongs to the PDGF/VEGF growth factor family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

References

1). Electroacupuncture facilitates implantation by enhancing endometrial angiogenesis in a rat model of ovarian hyperstimulation. Biology of Reproduction, 2019 (PubMed: 30084973) [IF=3.6]

Application: WB    Species: rat    Sample: DBA+ uNK cells

Figure 3 | The percentage of DBA+ uNK cells and PGF and DLL-1 expression in the endometrium. Flow cytometric analysis of the percentage of DBA+ uNK cells in the normal (N), model (M), electroacupuncture (EA) and electroacupuncture pretreatment (PEA) groups on the 4th day (D4), 6th day (D6) and 8th day (D8) of gestation (A). The percentage of DBA+ uNK cells in each the group was counted on D4 (B), D6 (C) and D8 (D). The protein expression of PGF and DLL-1 was detected by Western blot in each group on D4 (E), D6 (F) and D8 (G).

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