NARG1 Antibody - #AF0528

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Product: NARG1 Antibody
Catalog: AF0528
Description: Rabbit polyclonal antibody to NARG1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 100kDa; 101kD(Calculated).
Uniprot: Q9BXJ9
RRID: AB_2833796

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
NARG1 Antibody detects endogenous levels of total NARG1.
RRID:
AB_2833796
Cite Format: Affinity Biosciences Cat# AF0528, RRID:AB_2833796.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5730450D16Rik; 6330400I15; ASTBDN; GA19; Gastric cancer antigen Ga19; MGC29428; mNAT1; N alpha acetyltransferase 15, NatA auxiliary subunit; N terminal acetyltransferase; N(alpha) acetyltransferase 15, NatA auxiliary subunit; N-alpha-acetyltransferase 15; N-terminal acetyltransferase; Naa15; NAA15_HUMAN; NatA auxiliary subunit; NATH; NMDA receptor regulated 1; NMDA receptor regulated protein 1; NMDA receptor-regulated protein 1; Protein tubedown 1; Protein tubedown-1; Tbdn 1; Tbdn100; Transcriptional coactivator tubedown 100; tubedown-1;

Immunogens

Immunogen:
Uniprot:

Q9BXJ9(NAA15_HUMAN) >>Visit HPA database.

Gene(ID):
NAA15(80155)
Expression:
Q9BXJ9 NAA15_HUMAN:

Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy.

Description:
This gene encodes a protein of unknown function. However, similarity to proteins in yeast and other species suggests that this protein may be an N-acetyltransferase.
Sequence:
MPAVSLPPKENALFKRILRCYEHKQYRNGLKFCKQILSNPKFAEHGETLAMKGLTLNCLGKKEEAYELVRRGLRNDLKSHVCWHVYGLLQRSDKKYDEAIKCYRNALKWDKDNLQILRDLSLLQIQMRDLEGYRETRYQLLQLRPAQRASWIGYAIAYHLLEDYEMAAKILEEFRKTQQTSPDKVDYEYSELLLYQNQVLREAGLYREALEHLCTYEKQICDKLAVEETKGELLLQLCRLEDAADVYRGLQERNPENWAYYKGLEKALKPANMLERLKIYEEAWTKYPRGLVPRRLPLNFLSGEKFKECLDKFLRMNFSKGCPPVFNTLRSLYKDKEKVAIIEELVVGYETSLKSCRLFNPNDDGKEEPPTTLLWVQYYLAQHYDKIGQPSIALEYINTAIESTPTLIELFLVKAKIYKHAGNIKEAARWMDEAQALDTADRFINSKCAKYMLKANLIKEAEEMCSKFTREGTSAVENLNEMQCMWFQTECAQAYKAMNKFGEALKKCHEIERHFIEITDDQFDFHTYCMRKITLRSYVDLLKLEDVLRQHPFYFKAARIAIEIYLKLHDNPLTDENKEHEADTANMSDKELKKLRNKQRRAQKKAQIEEEKKNAEKEKQQRNQKKKKDDDDEEIGGPKEELIPEKLAKVETPLEEAIKFLTPLKNLVKNKIETHLFAFEIYFRKEKFLLMLQSVKRAFAIDSSHPWLHECMIRLFNTAVCESKDLSDTVRTVLKQEMNRLFGATNPKNFNETFLKRNSDSLPHRLSAAKMVYYLDPSSQKRAIELATTLDESLTNRNLQTCMEVLEALYDGSLGDCKEAAEIYRANCHKLFPYALAFMPPGYEEDMKITVNGDSSAEAEELANEI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BXJ9 As Substrate

Site PTM Type Enzyme
K9 Ubiquitination
K34 Acetylation
K34 Ubiquitination
K41 Acetylation
K41 Ubiquitination
K52 Ubiquitination
K61 Ubiquitination
K62 Acetylation
K62 Ubiquitination
Y66 Phosphorylation
Y86 Phosphorylation
K94 Ubiquitination
K101 Ubiquitination
T177 Phosphorylation
T180 Phosphorylation
K184 Ubiquitination
K223 Ubiquitination
K230 Ubiquitination
Y247 Phosphorylation
K262 Acetylation
K262 Ubiquitination
K266 Ubiquitination
K269 Ubiquitination
K278 Ubiquitination
Y280 Phosphorylation
K286 Ubiquitination
S302 Phosphorylation
K307 Ubiquitination
K312 Ubiquitination
K419 Ubiquitination
K425 Ubiquitination
R442 Methylation
K447 Ubiquitination
K454 Ubiquitination
K459 Ubiquitination
K467 Ubiquitination
K496 Ubiquitination
S537 Phosphorylation
Y538 Phosphorylation
K543 Ubiquitination
T584 Phosphorylation
S588 Phosphorylation
K646 Ubiquitination
K649 Ubiquitination
K659 Ubiquitination
T662 Phosphorylation
K665 Ubiquitination
C721 S-Nitrosylation
K724 Acetylation
K724 Ubiquitination
K735 Acetylation
K735 Ubiquitination
K748 Acetylation
K756 Acetylation
K756 Methylation
K756 Ubiquitination
S759 Phosphorylation
K770 Ubiquitination
K781 Ubiquitination
T850 Phosphorylation
S855 Phosphorylation
S856 Phosphorylation

Research Backgrounds

Function:

Auxillary subunit of the N-terminal acetyltransferase A (NatA) complex which displays alpha (N-terminal) acetyltransferase activity. The NAT activity may be important for vascular, hematopoietic and neuronal growth and development. Required to control retinal neovascularization in adult ocular endothelial cells. In complex with XRCC6 and XRCC5 (Ku80), up-regulates transcription from the osteocalcin promoter.

PTMs:

Cleaved by caspases during apoptosis, resulting in a stable 35 kDa fragment.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Mainly cytoplasmic, nuclear in some cases. Present in the free cytosolic and cytoskeleton-bound polysomes, but not in the membrane-bound polysomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy.

Subunit Structure:

Component of the N-terminal acetyltransferase A (NatA) complex composed of NAA10 or probably NAA11 and NAA15. Interacts with XRCC6, NAA50 and XRCC5. Associates with HYPK when in a complex with NAA10. Interaction with HYPK reduces the capacity to interact with NAA50.

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