DNA Polymerase beta Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using POLB Antibody.

Product:	DNA Polymerase beta Antibody
Catalog:	DF6552
Description:	Rabbit polyclonal antibody to DNA Polymerase beta
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	38kDa; 38kD(Calculated).
Uniprot:	P06746
RRID:	AB_2838514

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

DNA Polymerase beta Antibody detects endogenous levels of total DNA Polymerase beta.

RRID:

AB_2838514
Cite Format: Affinity Biosciences Cat# DF6552, RRID:AB_2838514.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

DNA directed DNA polymerase beta; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DPOLB_HUMAN; MGC125976; Pol B; Pol beta; POLB; Polymerase (DNA directed) beta;

Immunogens

Immunogen:

Uniprot:

P06746(DPOLB_HUMAN) >>Visit HPA database.

Gene(ID):

POLB(5423)

Description:

The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]

Sequence:

P06746 DPOLB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Horse

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P06746 As Substrate

P06746

Site	PTM Type	Enzyme	Source
	Ubiquitination		Uniprot
Y36	Phosphorylation		Uniprot
Y39	Phosphorylation		Uniprot
K41	Ubiquitination		Uniprot
S44	Phosphorylation		Uniprot
Y49	Phosphorylation		Uniprot
S55	Phosphorylation		Uniprot
K61	Ubiquitination		Uniprot
T67	Phosphorylation		Uniprot
K72	Acetylation	PR:000007102 (EP300)	Uniprot
K81	Acetylation		Uniprot
K81	Ubiquitination		Uniprot
R83	Methylation		Uniprot
K113	Ubiquitination		Uniprot
K141	Sumoylation		Uniprot
K141	Ubiquitination		Uniprot
K148	Ubiquitination		Uniprot
R152	Methylation		Uniprot
S180	Phosphorylation		Uniprot
K206	Ubiquitination		Uniprot
K230	Ubiquitination		Uniprot
K244	Ubiquitination		Uniprot
Y250	Phosphorylation		Uniprot
R258	Methylation		Uniprot
Y322	Phosphorylation		Uniprot

Research Backgrounds

P06746_DPOLB_HUMAN

Function:

Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.

PTMs:

Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.

Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation.

Subcellular Location:

Nucleus. Cytoplasm.
Note: Cytoplasmic in normal conditions. Translocates to the nucleus following DNA damage.

Subunit Structure:

Monomer. Interacts with APEX1, HUWE1/ARF-BP1, STUB1/CHIP and USP47. Interacts with FAM168A.

Family&Domains:

Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity.

Belongs to the DNA polymerase type-X family.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

Restrictive clause

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For Research Use Only.
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DNA Polymerase beta Antibody - #DF6552