KNG1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from 293, using KNG1 Antibody.

DF6544 at 1/100 staining Mouse liver tissue by IHC-P.

Figure 8 Protein expressions of JAM-A and Kng1 among the groups.

Product:	KNG1 Antibody
Catalog:	DF6544
Description:	Rabbit polyclonal antibody to KNG1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	72kDa; 72kD(Calculated).
Uniprot:	P01042
RRID:	AB_2838506

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

KNG1 Antibody detects endogenous levels of total KNG1.

RRID:

AB_2838506
Cite Format: Affinity Biosciences Cat# DF6544, RRID:AB_2838506.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Alpha-2-thiol proteinase inhibitor; BDK; BK; Bradykinin; Bradykinin included; Fitzgerald factor; FLAUJEAC FACTOR; High molecular weight kininogen; HMWK; Ile-Ser-Bradykinin; Kallidin I; Kallidin II; KNG; KNG1; KNG1_HUMAN; Low molecular weight growth-promoting factor; WILLIAMS FACTOR; Williams-Fitzgerald-Flaujeac factor;

Immunogens

Immunogen:

Uniprot:

P01042(KNG1_HUMAN) >>Visit HPA database.

Gene(ID):

KNG1(3827)

Expression:

P01042 KNG1_HUMAN:

Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.

Description:

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This gene is translated into High-molecular weight kininogen (HMWK) and low-molecular weight kininogen (LMWK) after alternative splicing. HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein. 11926-1-AP was generated against N-terminal 300 aa of HMW kininogen. It can bind both HMW and LMW kiniogen.

Sequence:

P01042 KNG1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKLITILFLCSRLLLSLTQESQSEEIDCNDKDLFKAVDAALKKYNSQNQSNNQFVLYRITEATKTVGSDTFYSFKYEIKEGDCPVQSGKTWQDCEYKDAAKAATGECTATVGKRSSTKFSVATQTCQITPAEGPVVTAQYDCLGCVHPISTQSPDLEPILRHGIQYFNNNTQHSSLFMLNEVKRAQRQVVAGLNFRITYSIVQTNCSKENFLFLTPDCKSLWNGDTGECTDNAYIDIQLRIASFSQNCDIYPGKDFVQPPTKICVGCPRDIPTNSPELEETLTHTITKLNAENNATFYFKIDNVKKARVQVVAGKKYFIDFVARETTCSKESNEELTESCETKKLGQSLDCNAEVYVVPWEKKIYPTVNCQPLGMISLMKRPPGFSPFRSSRIGEIKEETTVSPPHTSMAPAQDEERDSGKEQGHTRRHDWGHEKQRKHNLGHGHKHERDQGHGHQRGHGLGHGHEQQHGLGHGHKFKLDDDLEHQGGHVLDHGHKHKHGHGHGKHKNKGKKNGKHNGWKTEHLASSSEDSTTPSAQTQEKTEGPTPIPSLAKPGVTVTFSDFQDSDLIATMMPPISPAPIQSDDDWIPDIQIDPNGLSFNPISDFPDTTSPKCPGRPWKSVSEINPTTQMKESYYFDLTDGLS

PTMs - P01042 As Substrate

P01042

Site	PTM Type	Source
N48	N-Glycosylation	Uniprot
T110	Phosphorylation	Uniprot
K113	Acetylation	Uniprot
N169	N-Glycosylation	Uniprot
T171	Phosphorylation	Uniprot
S174	Phosphorylation	Uniprot
S175	Phosphorylation	Uniprot
N205	N-Glycosylation	Uniprot
S275	Phosphorylation	Uniprot
N294	N-Glycosylation	Uniprot
T326	Phosphorylation	Uniprot
T327	Phosphorylation	Uniprot
S329	Phosphorylation	Uniprot
S332	Phosphorylation	Uniprot
T337	Phosphorylation	Uniprot
T342	Phosphorylation	Uniprot
S386	Phosphorylation	Uniprot
S390	Phosphorylation	Uniprot
S391	Phosphorylation	Uniprot
T533	O-Glycosylation	Uniprot
T546	O-Glycosylation	Uniprot

Research Backgrounds

P01042_KNG1_HUMAN

Function:

(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

PTMs:

Bradykinin is released from kininogen by plasma kallikrein.

Hydroxylation of Pro-383 occurs prior to the release of bradykinin.

Phosphorylated by FAM20C in the extracellular medium.

N- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans.

Subcellular Location:

Secreted>Extracellular space.

Tissue Specificity:

Secreted in plasma. T-kinin is detected in malignant ovarian, colon and breast carcinomas, but not in benign tumors.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

References

1). Tandem Mass Tag Analysis of the Effect of the Anterior Cingulate Cortex in Nonerosive Reflux Disease Rats with Shugan Jiangni Hewei Granules Treatment. Computational and Mathematical Methods in Medicine, 2022 (PubMed: 35941898)

Application: WB Species: Rat Sample:

Figure 8 Protein expressions of JAM-A and Kng1 among the groups. The expression level of JAM-A and Kng1 were analyzed through western blot analysis. ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05.

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KNG1 Antibody - #DF6544