UGT1A9 Antibody - #DF6537

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Product: UGT1A9 Antibody
Catalog: DF6537
Description: Rabbit polyclonal antibody to UGT1A9
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 60kDa; 60kD(Calculated).
Uniprot: O60656
RRID: AB_2838499

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
UGT1A9 Antibody detects endogenous levels of total UGT1A9.
RRID:
AB_2838499
Cite Format: Affinity Biosciences Cat# DF6537, RRID:AB_2838499.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GNT1; HLUGP4; lugP4; UD19_HUMAN; UDP glucuronosyltransferase 1A9; UDP glucuronosyltransferase family 1 member A9; UDP-glucuronosyltransferase 1 family, polypeptide A9; UDP-glucuronosyltransferase 1-9; UDP-glucuronosyltransferase 1-I; UDP-glucuronosyltransferase 1A9; UDPGT 1-9; UDPGT; UGT-1I; UGT1*9; UGT1-09; UGT1.9; Ugt1a9; UGT1A9S; UGT1AI; UGT1I;

Immunogens

Immunogen:
Uniprot:

O60656(UD19_HUMAN) >>Visit HPA database.

Gene(ID):
UGT1A9(54600)
Expression:
O60656 UD19_HUMAN:

Liver. Isoform 1 and isoform 2 are expressed in liver, kidney, colon, esophagus and small intestine.

Description:
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenols. [provided by RefSeq, Jul 2008]
Sequence:
MACTGWTSPLPLCVCLLLTCGFAEAGKLLVVPMDGSHWFTMRSVVEKLILRGHEVVVVMPEVSWQLGRSLNCTVKTYSTSYTLEDLDREFKAFAHAQWKAQVRSIYSLLMGSYNDIFDLFFSNCRSLFKDKKLVEYLKESSFDAVFLDPFDNCGLIVAKYFSLPSVVFARGILCHYLEEGAQCPAPLSYVPRILLGFSDAMTFKERVRNHIMHLEEHLLCHRFFKNALEIASEILQTPVTEYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH

PTMs - O60656 As Substrate

Site PTM Type Enzyme
N71 N-Glycosylation
T202 Phosphorylation
N292 N-Glycosylation
N344 N-Glycosylation
K434 Acetylation

Research Backgrounds

Function:

UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform has specificity for phenols. Isoform 2 lacks transferase activity but acts as a negative regulator of isoform 1.

Subcellular Location:

Microsome. Endoplasmic reticulum membrane>Single-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Liver. Isoform 1 and isoform 2 are expressed in liver, kidney, colon, esophagus and small intestine.

Subunit Structure:

Isoform 1 interacts with isoform 2/i2 suggesting that oligomerization is involved in negative regulation of transferase activity by isoform 2. Isoform 1 also interacts with respective i2 isoforms of UGT1A1, UGT1A3, UGT1A4, UGT1A6, UGT1A7, UGT1A8 and UGT1A10.

Family&Domains:

Belongs to the UDP-glycosyltransferase family.

Research Fields

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Metabolism > Carbohydrate metabolism > Pentose and glucuronate interconversions.

· Metabolism > Carbohydrate metabolism > Ascorbate and aldarate metabolism.

· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Porphyrin and chlorophyll metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). A UPLC-MS/MS method reveals the pharmacokinetics and metabolism characteristics of kaempferol in rats under hypoxia. Drug Metabolism and Pharmacokinetics, 2022 (PubMed: 35051732) [IF=2.1]

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