Syndecan 4 Antibody - #AF0831

(2)   (5)  
Product: Syndecan 4 Antibody
Catalog: AF0831
Description: Rabbit polyclonal antibody to Syndecan 4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 22kDa; 22kD(Calculated).
Uniprot: P31431
RRID: AB_2833791

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(86%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
Syndecan 4 Antibody detects endogenous levels of total Syndecan 4.
RRID:
AB_2833791
Cite Format: Affinity Biosciences Cat# AF0831, RRID:AB_2833791.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Amphiglycan; MGC22217; OTTHUMP00000031788; ryudocan amphiglycan; Ryudocan; Ryudocan core protein; SDC 4; Sdc4; SDC4_HUMAN; SYND 4; SYND4; syndecan 4; syndecan 4 (amphiglycan, ryudocan); syndecan proteoglycan 4; Syndecan-4; Syndecan4;

Immunogens

Immunogen:
Uniprot:

P31431(SDC4_HUMAN) >>Visit HPA database.

Gene(ID):
SDC4(6385)
Expression:
P31431 SDC4_HUMAN:

Expressed in epithelial and fibroblastic cells.

Description:
The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. The encoded protein is found as a homodimer and is a member of the syndecan proteoglycan family. This gene is found on chromosome 20, while a pseudogene has been found on chromosome 22. SwissProt Information
Sequence:
MAPARLFALLLFFVGGVAESIRETEVIDPQDLLEGRYFSGALPDDEDVVGPGQESDDFELSGSGDLDDLEDSMIGPEVVHPLVPLDNHIPERAGSGSQVPTEPKKLEENEVIPKRISPVEESEDVSNKVSMSSTVQGSNIFERTEVLAALIVGGIVGILFAVFLILLLMYRMKKKDEGSYDLGKKPIYKKAPTNEFYA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Zebrafish
100
Chicken
100
Dog
86
Rabbit
86
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31431 As Substrate

Site PTM Type Enzyme
S97 O-Glycosylation
T101 O-Glycosylation
K104 Ubiquitination
S117 O-Glycosylation
S126 O-Glycosylation
T134 Phosphorylation
K174 Ubiquitination
S179 Phosphorylation Q05655 (PRKCD)
Y180 Phosphorylation P12931 (SRC)
K190 Ubiquitination
T193 Phosphorylation
Y197 Phosphorylation

Research Backgrounds

Function:

Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.

PTMs:

Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.

Subcellular Location:

Membrane>Single-pass type I membrane protein. Secreted.
Note: Shedding of the ectodomain produces a soluble form.

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in epithelial and fibroblastic cells.

Subunit Structure:

Homodimer. Interacts (via its cytoplasmic domain) with GIPC (via its PDZ domain). Interacts (via its cytoplasmic domain) with NUDT16L1 (By similarity). Interacts with CDCP1 and SDCBP.

Family&Domains:

Belongs to the syndecan proteoglycan family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > ECM-receptor interaction.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

References

1). Necroptotic TNFα‐syndecan 4‐TNFα vicious cycle as a therapeutic target for preventing temporomandibular joint osteoarthritis. Journal of Bone and Mineral Research, 2022 (PubMed: 35278225) [IF=6.2]

Application: IHC    Species: Rat    Sample:

Fig. 5 SDC4 as specific necroptotic DAMPs contributing to cartilage degeneration and synovial inflammation. (A) Cell morphology was explored by interference light microscopy after treatment with different agents. (B) TNFα, ADAMTS5, and MMP13 in the culture supernatants in different groups were measured by ELISA (n = 4). (C) Western blot analysis of TNFα, ADAMTS5, MMP13, ACAN, RIPK3, and pMLKL in the different groups. (D) Quantitative data of C (n = 3). (E) IHC staining of SDC4, RIPK3, and pMLKL in different groups. (F) Quantitative data of E (n = 6). (G) H&E staining and TNFα-IHC staining of cartilage and synovium in different groups. (H) Quantitative data of G. (n = 6). Scale bars = 100 μm. Unpaired t test was used to compare the data between two groups.

Application: WB    Species: Rat    Sample:

Fig. 5 SDC4 as specific necroptotic DAMPs contributing to cartilage degeneration and synovial inflammation. (A) Cell morphology was explored by interference light microscopy after treatment with different agents. (B) TNFα, ADAMTS5, and MMP13 in the culture supernatants in different groups were measured by ELISA (n = 4). (C) Western blot analysis of TNFα, ADAMTS5, MMP13, ACAN, RIPK3, and pMLKL in the different groups. (D) Quantitative data of C (n = 3). (E) IHC staining of SDC4, RIPK3, and pMLKL in different groups. (F) Quantitative data of E (n = 6). (G) H&E staining and TNFα-IHC staining of cartilage and synovium in different groups. (H) Quantitative data of G. (n = 6). Scale bars = 100 μm. Unpaired t test was used to compare the data between two groups.
2). Necroptotic TNFα-Syndecan 4-TNFα Vicious Cycle as a Therapeutic Target for Preventing Temporomandibular Joint Osteoarthritis. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2022 (PubMed: 35278225) [IF=6.2]

Application: IHC    Species: Rat    Sample:

Fig. 5. SDC4 as specific necroptotic DAMPs contributing to cartilage degeneration and synovial inflammation. (A) Cell morphology was explored by inter-ference light microscopy after treatment with different agents. (B) TNFα, ADAMTS5, and MMP13 in the culture supernatants in different groups were mea-sured by ELISA (n = 4). (C) Western blot analysis of TNFα, ADAMTS5, MMP13, ACAN, RIPK3, and pMLKL in the different groups. (D) Quantitative data ofC (n = 3). (E) IHC staining of SDC4, RIPK3, and pMLKL in different groups. (F) Quantitative data of E (n = 6). (G) H&E staining and TNFα-IHC staining of car-tilage and synovium in different groups. (H) Quantitative data of G. (n = 6). Scale bars = 100 μm. Unpaired t test was used to compare the data betweentwo groups.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.