ETV6 / Tel Antibody | Affinity Biosciences

1/2

Western blot analysis of extracts from A431, using ETV6 antibody.

DF6521 at 1/100 staining Human pancreatic cancer by IHC-P.

Product:	ETV6 / Tel Antibody
Catalog:	DF6521
Description:	Rabbit polyclonal antibody to ETV6 / Tel
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	53kDa; 53kD(Calculated).
Uniprot:	P41212
RRID:	AB_2838483

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(88%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

ETV6 / Tel Antibody detects endogenous levels of total ETV6 / Tel.

RRID:

AB_2838483
Cite Format: Affinity Biosciences Cat# DF6521, RRID:AB_2838483.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ETS related protein Tel1; ETS translocation variant 6; Ets variant 6; Ets variant gene 6 (TEL oncogene); ETS-related protein Tel1; ETV 6; ETV6; ETV6_HUMAN; Tel; TEL oncogene; TEL/ABL; TEL1; TEL1 oncogene; Transcription factor ETV 6; Transcription factor ETV6;

Immunogens

Immunogen:

Uniprot:

P41212(ETV6_HUMAN) >>Visit HPA database.

Gene(ID):

ETV6(2120)

Expression:

P41212 ETV6_HUMAN:

Ubiquitous.

Description:

This gene encodes an ETS family transcription factor. The product of this gene contains two functional domains: a N-terminal pointed (PNT) domain that is involved in protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain. Gene knockout studies in mice suggest that it is required for hematopoiesis and maintenance of the developing vascular network. This gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma. [provided by RefSeq, Sep 2008]

Sequence:

P41212 ETV6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSETPAQCSIKQERISYTPPESPVPSYASSTPLHVPVPRALRMEEDSIRLPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNTFEMNGKALLLLTKEDFRYRSPHSGDVLYELLQHILKQRKPRILFSPFFHPGNSIHTQPEVILHQNHEEDNCVQRTPRPSVDNVHHNPPTIELLHRSRSPITTNHRPSPDPEQRPLRSPLDNMIRRLSPAERAQGPRPHQENNHQESYPLSVSPMENNHCPASSESHPKPSSPRQESTRVIQLMPSPIMHPLILNPRHSVDFKQSRLSEDGLHREGKPINLSHREDLAYMNHIMVSVSPPEEHAMPIGRIADCRLLWDYVYQLLSDSRYENFIRWEDKESKIFRIVDPNGLARLWGNHKNRTNMTYEKMSRALRHYYKLNIIRKEPGQRLLFRFMKTPDEIMSGRTDRLEHLESQELDEQIYQEDEC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Dog

100

Chicken

100

Rabbit

100

Bovine

Xenopus

Zebrafish

Horse

Sheep

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41212 As Substrate

P41212

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
T4	Phosphorylation		Uniprot
K11	Acetylation		Uniprot
K11	Sumoylation		Uniprot
S16	Phosphorylation		Uniprot
Y17	Phosphorylation		Uniprot
T18	Phosphorylation		Uniprot
S22	Phosphorylation	Q16539 (MAPK14)	Uniprot
S26	Phosphorylation		Uniprot
Y27	Phosphorylation		Uniprot
S29	Phosphorylation		Uniprot
S30	Phosphorylation		Uniprot
T31	Phosphorylation		Uniprot
S47	Phosphorylation		Uniprot
Y60	Phosphorylation		Uniprot
K99	Sumoylation		Uniprot
S131	Phosphorylation		Uniprot
S139	Phosphorylation		Uniprot
T142	Phosphorylation		Uniprot
T161	Phosphorylation		Uniprot
S165	Phosphorylation		Uniprot
S182	Phosphorylation		Uniprot
S184	Phosphorylation		Uniprot
T187	Phosphorylation		Uniprot
T188	Phosphorylation		Uniprot
S193	Phosphorylation		Uniprot
S203	Phosphorylation		Uniprot
S213	Phosphorylation	P27361 (MAPK3)	Uniprot
S238	Phosphorylation		Uniprot
S257	Phosphorylation	Q16539 (MAPK14) , P27361 (MAPK3)	Uniprot
S271	Phosphorylation		Uniprot
S284	Phosphorylation		Uniprot
S293	Phosphorylation		Uniprot
K302	Acetylation		Uniprot
K302	Ubiquitination		Uniprot
Y314	Phosphorylation		Uniprot
S323	Phosphorylation		Uniprot
K384	Ubiquitination		Uniprot
K393	Ubiquitination		Uniprot
Y401	Phosphorylation		Uniprot
Y402	Phosphorylation		Uniprot
K403	Ubiquitination		Uniprot
K421	Sumoylation		Uniprot
K421	Ubiquitination		Uniprot
T422	Phosphorylation		Uniprot
S439	Phosphorylation		Uniprot
Y447	Phosphorylation		Uniprot

Research Backgrounds

P41212_ETV6_HUMAN

Function:

Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'. Plays a role in hematopoiesis and malignant transformation.

PTMs:

Phosphorylation of Ser-257 by MAPK14 (p38) inhibits ETV6 transcriptional repression.

Subcellular Location:

Nucleus.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Can form homodimers or heterodimers with TEL2 or FLI1. Interacts with L3MBTL1 and HDAC9.

Family&Domains:

Belongs to the ETS family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

ETV6 / Tel Antibody - #DF6521