Insulin degrading enzyme/IDE Antibody - #DF6515

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Product: Insulin degrading enzyme/IDE Antibody
Catalog: DF6515
Description: Rabbit polyclonal antibody to Insulin degrading enzyme/IDE
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 118kDa; 118kD(Calculated).
Uniprot: P14735
RRID: AB_2838477

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
Insulin degrading enzyme/IDE Antibody detects endogenous levels of total Insulin degrading enzyme/IDE.
RRID:
AB_2838477
Cite Format: Affinity Biosciences Cat# DF6515, RRID:AB_2838477.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Abeta-degrading protease; FLJ35968; Ide; IDE_HUMAN; Insulin protease; Insulin-degrading enzyme; Insulinase; Insulysin; OTTHUMP00000020097;

Immunogens

Immunogen:
Uniprot:

P14735(IDE_HUMAN) >>Visit HPA database.

Gene(ID):
IDE(3416)
Expression:
P14735 IDE_HUMAN:

Detected in brain and in cerebrospinal fluid (at protein level).

Description:
This gene encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of this enzyme for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in this protein's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described but have not been experimentally verified.[provided by RefSeq, Sep 2009]
Sequence:
MRYRLAWLLHPALPSTFRSVLGARLPPPERLCGFQKKTYSKMNNPAIKRIGNHITKSPEDKREYRGLELANGIKVLLISDPTTDKSSAALDVHIGSLSDPPNIAGLSHFCEHMLFLGTKKYPKENEYSQFLSEHAGSSNAFTSGEHTNYYFDVSHEHLEGALDRFAQFFLCPLFDESCKDREVNAVDSEHEKNVMNDAWRLFQLEKATGNPKHPFSKFGTGNKYTLETRPNQEGIDVRQELLKFHSAYYSSNLMAVCVLGRESLDDLTNLVVKLFSEVENKNVPLPEFPEHPFQEEHLKQLYKIVPIKDIRNLYVTFPIPDLQKYYKSNPGHYLGHLIGHEGPGSLLSELKSKGWVNTLVGGQKEGARGFMFFIINVDLTEEGLLHVEDIILHMFQYIQKLRAEGPQEWVFQECKDLNAVAFRFKDKERPRGYTSKIAGILHYYPLEEVLTAEYLLEEFRPDLIEMVLDKLRPENVRVAIVSKSFEGKTDRTEEWYGTQYKQEAIPDEVIKKWQNADLNGKFKLPTKNEFIPTNFEILPLEKEATPYPALIKDTAMSKLWFKQDDKFFLPKACLNFEFFSPFAYVDPLHCNMAYLYLELLKDSLNEYAYAAELAGLSYDLQNTIYGMYLSVKGYNDKQPILLKKIIEKMATFEIDEKRFEIIKEAYMRSLNNFRAEQPHQHAMYYLRLLMTEVAWTKDELKEALDDVTLPRLKAFIPQLLSRLHIEALLHGNITKQAALGIMQMVEDTLIEHAHTKPLLPSQLVRYREVQLPDRGWFVYQQRNEVHNNCGIEIYYQTDMQSTSENMFLELFCQIISEPCFNTLRTKEQLGYIVFSGPRRANGIQGLRFIIQSEKPPHYLESRVEAFLITMEKSIEDMTEEAFQKHIQALAIRRLDKPKKLSAECAKYWGEIISQQYNFDRDNTEVAYLKTLTKEDIIKFYKEMLAVDAPRRHKVSVHVLAREMDSCPVVGEFPCQNDINLSQAPALPQPEVIQNMTEFKRGLPLFPLVKPHINFMAAKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14735 As Substrate

Site PTM Type Enzyme
Ubiquitination
T55 Phosphorylation
S57 Phosphorylation
K119 Ubiquitination
K192 Acetylation
K192 Ubiquitination
K206 Acetylation
K206 Ubiquitination
K212 Ubiquitination
K217 Ubiquitination
T220 Phosphorylation
K223 Ubiquitination
S276 Phosphorylation
K281 Ubiquitination
K299 Ubiquitination
K308 Acetylation
K308 Ubiquitination
K501 Ubiquitination
K511 Ubiquitination
K512 Acetylation
K512 Ubiquitination
K521 Acetylation
K527 Acetylation
K527 Ubiquitination
K542 Ubiquitination
K558 Ubiquitination
K566 Ubiquitination
Y625 Phosphorylation
K657 Ubiquitination
T691 Phosphorylation
T696 Phosphorylation
K697 Ubiquitination
K701 Ubiquitination
K713 Ubiquitination
R774 Methylation
K826 Ubiquitination
K854 Ubiquitination
K884 Ubiquitination
K899 Ubiquitination
S901 Phosphorylation
K929 Ubiquitination
K933 Ubiquitination
K938 Ubiquitination
S955 Phosphorylation
K1018 Ubiquitination

Research Backgrounds

Function:

Plays a role in the cellular breakdown of insulin, APP peptides, IAPP peptides, glucagon, bradykinin, kallidin and other peptides, and thereby plays a role in intercellular peptide signaling. Substrate binding induces important conformation changes, making it possible to bind and degrade larger substrates, such as insulin. Contributes to the regulation of peptide hormone signaling cascades and regulation of blood glucose homeostasis via its role in the degradation of insulin, glucagon and IAPP (By similarity). Plays a role in the degradation and clearance of APP-derived amyloidogenic peptides that are secreted by neurons and microglia (Probable). Involved in antigen processing. Produces both the N terminus and the C terminus of MAGEA3-derived antigenic peptide (EVDPIGHLY) that is presented to cytotoxic T lymphocytes by MHC class I.

(Microbial infection) The membrane-associated isoform acts as an entry receptor for varicella-zoster virus (VZV).

PTMs:

The N-terminus is blocked.

Subcellular Location:

Cytoplasm>Cytosol. Cell membrane. Secreted.
Note: Present at the cell surface of neuron cells. The membrane-associated isoform is approximately 5 kDa larger than the known cytosolic isoform.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in brain and in cerebrospinal fluid (at protein level).

Subunit Structure:

Homodimer (Probable). Can also form homotetramers (By similarity).

(Microbial infection) Interacts (via N-terminus) with varicella-zoster virus (VZV) envelope glycoprotein E (via N-terminus); the membrane-associated isoform may function as an entry receptor for this virus.

Family&Domains:

The SlyX motif may be involved in the non-conventional secretion of the protein.

Belongs to the peptidase M16 family.

Research Fields

· Human Diseases > Neurodegenerative diseases > Alzheimer's disease.

References

1). Lovastatin attenuates sevoflurane-induced cognitive disorder in aged rats via reducing Aβ accumulation. NEUROCHEMISTRY INTERNATIONAL, 2021 (PubMed: 34048842) [IF=4.2]

Application: WB    Species: rat    Sample: BV-2 cells

Fig. 2.| Lovastatin increased IDE-enriched exosome secretion in sevoflurane-exposed BV-2 cells. (A) The mRNA level of IDE in BV-2 cells analyzed by Realtime PCR. (B) CD63 expression detected by flow cytometry. (C) IDE protein level in BV-2 cellderived exosomes.

Application: WB    Species: rat    Sample: BV-2 cell

Fig. 2.| Lovastatin increased IDE-enriched exosome secretion in sevoflurane-exposed BV-2 cells. (A) The mRNA level of IDE in BV-2 cells analyzed by Realtime PCR. (B) CD63 expression detected by flow cytometry. (C) IDE protein level in BV-2 cell derived exosomes.

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