GSTA1 Antibody | Affinity Biosciences

WB
IHC
Cites

1/5

Western blot analysis of extracts from 293, using GSTA1 Antibody.

DF6514 at 1/100 staining Rat liver tissue by IHC-P.

Fig.

FIGURE 6| A, Original blots for HNF‐1, GSTA1 and β‐actin.

FIGURE 7 | Changes of GSTA1 caused by different dosages of APAP in mice with or without SP600125.

Product:	GSTA1 Antibody
Catalog:	DF6514
Description:	Rabbit polyclonal antibody to GSTA1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Chicken, Xenopus
Mol.Wt.:	26kDa; 26kD(Calculated).
Uniprot:	P08263
RRID:	AB_2838476

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(91%), Horse(92%), Sheep(91%), Chicken(82%), Xenopus(91%)

Clonality:

Polyclonal

Specificity:

GSTA1 Antibody detects endogenous levels of total GSTA1.

RRID:

AB_2838476
Cite Format: Affinity Biosciences Cat# DF6514, RRID:AB_2838476.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Glutathione S alkyltransferase A1; Glutathione S aryltransferase A1; Glutathione S transferase 2; Glutathione S transferase A1; Glutathione S transferase alpha 1; Glutathione S transferase Ha subunit 1; Glutathione S-transferase A1; GST class-alpha member 1; GST epsilon; GST HA subunit 1; GST, class alpha, 1; GST-epsilon; GST2; GSTA1 1; GSTA1; GSTA1-1; GSTA1_HUMAN; GTH1; HA subunit 1; MGC131939; OTTHUMP00000016611; S (hydroxyalkyl)glutathione lyase A1;

Immunogens

Immunogen:

Uniprot:

P08263(GSTA1_HUMAN) >>Visit HPA database.

Gene(ID):

GSTA1(2938)

Expression:

P08263 GSTA1_HUMAN:

Liver.

Description:

Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation. [provided by RefSeq, Jul 2008]

Sequence:

P08263 GSTA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDIKERALIDMYIEGIADLGEMILLLPVCPPEEKDAKLALIKEKIKNRYFPAFEKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQPGSPRKPPMDEKSLEEARKIFRF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Pig

Bovine

Sheep

Xenopus

Chicken

Dog

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08263 As Substrate

P08263

Site	PTM Type	Source
Y9	Phosphorylation	Uniprot
S37	Phosphorylation	Uniprot
Y49	Phosphorylation	Uniprot
Y74	Phosphorylation	Uniprot
S77	Phosphorylation	Uniprot
Y79	Phosphorylation	Uniprot
Y82	Phosphorylation	Uniprot
K84	Acetylation	Uniprot
K87	Acetylation	Uniprot
K125	Ubiquitination	Uniprot
Y132	Phosphorylation	Uniprot
S142	Phosphorylation	Uniprot
Y147	Phosphorylation	Uniprot
K152	Acetylation	Uniprot
S154	Phosphorylation	Uniprot
S176	Phosphorylation	Uniprot
S177	Phosphorylation	Uniprot
S189	Phosphorylation	Uniprot
T193	Phosphorylation	Uniprot
K195	Acetylation	Uniprot
K196	Acetylation	Uniprot
S202	Phosphorylation	Uniprot
S212	Phosphorylation	Uniprot

Research Backgrounds

P08263_GSTA1_HUMAN

Function:

Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid.

Subcellular Location:

Cytoplasm.

Tissue Specificity:

Liver.

Subunit Structure:

Homodimer or heterodimer of GSTA1 and GSTA2.

Family&Domains:

The C-terminal domain may form a component of the hydrophobic substrate-binding site, but in contrast appears not to be directly involved in GSH binding and is not absolutely essential for catalytic activity.

Belongs to the GST superfamily. Alpha family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma. (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

References

1). JNK signaling pathway mediates acetaminophen-induced hepatotoxicity accompanied by changes of glutathione S-transferase A1 content and expression. Frontiers in Pharmacology (PubMed: 31620005) [IF=5.6]

Application: WB Species: mouse Sample: liver

FIGURE 7 | Changes of GSTA1 caused by different dosages of APAP in mice with or without SP600125. (C) Western blot analyses of total tissue lysate for GSTA1 and β-actin (loading control), n = 3.

2). Acetaminophen-induced hepatocyte injury: C2-ceramide and oltipraz intervention, hepatocyte nuclear factor 1 and glutathione S-transferase A1 changes. JOURNAL OF APPLIED TOXICOLOGY (PubMed: 31385618) [IF=3.3]

Application: WB Species: Sample: hepatocytes

FIGURE 6| A, Original blots for HNF‐1, GSTA1 and β‐actin. B, HNF‐1 protein level. C, GSTA1 protein level. Changes in HNF‐1 and GSTA1 protein levels in hepatocytes after 15 mmol/L APAP exposure for 10 h with C2 (6 μmol/L) or OL (8 μmol/L). Values are expressed as the mean ± SD for each group (n = 4). *P < .05, **P < .01 represented all groups compared with the control group; #P < .05, ##P < .01 represented all groups compared with the APAP model group. APAP, acetaminophen; C2, C2‐ceramide; GSTA1, glutathione S‐transferase A1; HNF‐1, hepatocyte nuclear factor 1; OL, oltipraz

3). Acetaminophen-induced reduction in glutathione-S-transferase A1 in hepatocytes: A role for hepatic nuclear factor 1α and its response element. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS (PubMed: 31230750) [IF=3.1]

Application: WB Species: human Sample: HepG2 cells

Fig. 3. |Effects of C2-ceramide or oltipraz on the nuclear HNF-1a levels and the GSTA1 protein expression in HepG2 cells with injury induced by APAP (n ¼ 4). *P < 0.05, **P < 0.01 compared with control group; #P < 0.05, ##P < 0.01 compared with model group.

4). Prevention of acetaminophen-induced hepatocyte injury: JNK inhibition and GSTA1 involvement. Molecular & Cellular Toxicology [IF=1.7]

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GSTA1 Antibody - #DF6514