XPA Antibody - #DF6513

(3)  
Product: XPA Antibody
Catalog: DF6513
Description: Rabbit polyclonal antibody to XPA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus
Mol.Wt.: 31kDa; 31kD(Calculated).
Uniprot: P23025
RRID: AB_2838475

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%), Zebrafish(88%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
XPA Antibody detects endogenous levels of total XPA.
RRID:
AB_2838475
Cite Format: Affinity Biosciences Cat# DF6513, RRID:AB_2838475.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA repair protein complementing XP A cells; DNA repair protein complementing XP-A cells; DNA repair protein complementing XPA cells; Excision repair controlling; Xeroderma pigmentosum 1; Xeroderma pigmentosum complementation group A; Xeroderma pigmentosum group A complementing protein; Xeroderma pigmentosum group A-complementing protein; XP 1; XP1; xpa; XPA_HUMAN; Xpac;

Immunogens

Immunogen:
Uniprot:

P23025(XPA_HUMAN) >>Visit HPA database.

Gene(ID):
XPA(7507)
Expression:
P23025 XPA_HUMAN:

Expressed in various cell lines and in skin fibroblasts.

Description:
This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
Sequence:
MAAADGALPEAAALEQPAELPASVRASIERKRQRALMLRQARLAARPYSATAAAATGGMANVKAAPKIIDTGGGFILEEEEEEEQKIGKVVHQPGPVMEFDYVICEECGKEFMDSYLMNHFDLPTCDNCRDADDKHKLITKTEAKQEYLLKDCDLEKREPPLKFIVKKNPHHSQWGDMKLYLKLQIVKRSLEVWGSQEALEEAKEVRQENREKMKQKKFDKKVKELRRAVRSSVWKRETIVHQHEYGPEENLEDDMYRKTCTMCGHELTYEKM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Pig
88
Xenopus
88
Zebrafish
88
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23025 As Substrate

Site PTM Type Enzyme
A2 Acetylation
Y48 Phosphorylation
K63 Sumoylation
K67 Ubiquitination
K86 Sumoylation
K141 Sumoylation
K141 Ubiquitination
K145 Sumoylation
K145 Ubiquitination
K151 Ubiquitination
K157 Acetylation
K168 Ubiquitination
S173 Phosphorylation P78527 (PRKDC) , Q13535 (ATR) , Q13315 (ATM)
S196 Phosphorylation P78527 (PRKDC) , Q13315 (ATM) , Q13535 (ATR)
K204 Ubiquitination
T239 Phosphorylation
Y257 Phosphorylation
K259 Ubiquitination

Research Backgrounds

Function:

Involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. Required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation.

PTMs:

ATR-dependent phosphorylation of XPA at Ser-196 is important for cell survival in response to UV damage.

Ubiquitinated by HERC2 leading to degradation by the proteasome.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in various cell lines and in skin fibroblasts.

Subunit Structure:

Interacts with GPN1. Interacts with RPA1 and RPA2; the interaction is direct and associates XPA with the RPA complex. Interacts (via N-terminus) with CEP164 upon UV irradiation. Interacts with HERC2.

Family&Domains:

Belongs to the XPA family.

Research Fields

· Genetic Information Processing > Replication and repair > Nucleotide excision repair.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

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