TFPI Antibody - #DF6511

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Product: TFPI Antibody
Catalog: DF6511
Description: Rabbit polyclonal antibody to TFPI
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Chicken
Mol.Wt.: 35kDa; 35kD(Calculated).
Uniprot: P10646
RRID: AB_2838473

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(86%), Bovine(86%), Horse(86%), Rabbit(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
TFPI Antibody detects endogenous levels of total TFPI.
RRID:
AB_2838473
Cite Format: Affinity Biosciences Cat# DF6511, RRID:AB_2838473.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Anti convertin; EPI; Extrinsic pathway inhibitor; LACI; Lipoprotein associated coagulation inhibitor; Lipoprotein-associated coagulation inhibitor; TFI; TFPI 1; TFPI; TFPI1; TFPI1_HUMAN; Tissue factor pathway inhibitor (lipoprotein associated coagulation inhibitor); Tissue factor pathway inhibitor;

Immunogens

Immunogen:
Uniprot:

P10646(TFPI1_HUMAN) >>Visit HPA database.

Gene(ID):
TFPI(7035)
Expression:
P10646 TFPI1_HUMAN:

Mostly in endothelial cells.

Description:
This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq, Jul 2008]
Sequence:
MIYTMKKVHALWASVCLLLNLAPAPLNADSEEDEEHTIITDTELPPLKLMHSFCAFKADDGPCKAIMKRFFFNIFTRQCEEFIYGGCEGNQNRFESLEECKKMCTRDNANRIIKTTLQQEKPDFCFLEEDPGICRGYITRYFYNNQTKQCERFKYGGCLGNMNNFETLEECKNICEDGPNGFQVDNYGTQLNAVNNSLTPQSTKVPSLFEFHGPSWCLTPADRGLCRANENRFYYNSVIGKCRPFKYSGCGGNENNFTSKQECLRACKKGFIQRISKGGLIKTKRKRKKQRVKIAYEEIFVKNM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
86
Horse
86
Bovine
86
Chicken
83
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P10646 As Substrate

Site PTM Type Enzyme
S30 Phosphorylation P68400 (CSNK2A1)
T42 O-Glycosylation
T115 O-Glycosylation
T116 O-Glycosylation
K121 Ubiquitination
N145 N-Glycosylation
N195 N-Glycosylation
S202 O-Glycosylation
S202 Phosphorylation P28482 (MAPK1)
T203 O-Glycosylation
T219 Phosphorylation
K260 Ubiquitination
K268 Acetylation
K269 Acetylation
K269 Sumoylation
K293 Sumoylation

Research Backgrounds

Function:

Inhibits factor X (X(a)) directly and, in a Xa-dependent way, inhibits VIIa/tissue factor activity, presumably by forming a quaternary Xa/LACI/VIIa/TF complex. It possesses an antithrombotic action and also the ability to associate with lipoproteins in plasma.

PTMs:

O-glycosylated.

Subcellular Location:

Secreted.

Microsome membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mostly in endothelial cells.

Family&Domains:

This inhibitor contains three inhibitory domains. The first domain interacts with VIIa and TF, the second one with Xa.

Research Fields

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

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