SELL Antibody | Affinity Biosciences

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Western blot analysis of Jurkat whole cell lysates, using SELL Antibody.

DF6509 at 1/100 staining Mouse brain tissue by IHC-P.

Product:	SELL Antibody
Catalog:	DF6509
Description:	Rabbit polyclonal antibody to SELL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	42kDa; 42kD(Calculated).
Uniprot:	P14151
RRID:	AB_2838471

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(91%), Horse(82%), Sheep(100%), Rabbit(91%), Dog(100%)

Clonality:

Polyclonal

Specificity:

SELL Antibody detects endogenous levels of total SELL.

RRID:

AB_2838471
Cite Format: Affinity Biosciences Cat# DF6509, RRID:AB_2838471.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A.11; AI528707; CD62 antigen ligand; CD62 antigen-like family member L; CD62L; gp90-MEL; IgA nephropathy, susceptibility to, included; L Selectin; L-selectin; LAM-1; LAM1; LECAM1; LEU8; Leukocyte adhesion molecule 1; Leukocyte surface antigen Leu-8; Leukocyte-endothelial cell adhesion molecule 1; Lnhr; LSEL; Ly-22; Ly-m22; Lyam-1; LYAM1; LYAM1_HUMAN; Lymph node homing receptor; Lymphocyte adhesion molecule 1; Lymphocyte antigen 22; Lymphocyte surface MEL-14 antigen; MEL-14; Pln homing receptor; PLNHR; Selectin L; Selectin, lymphocyte; SELL; TQ1;

Immunogens

Immunogen:

Uniprot:

P14151(LYAM1_HUMAN) >>Visit HPA database.

Gene(ID):

SELL(6402)

Expression:

P14151 LYAM1_HUMAN:

Expressed in B-cell lines and T-lymphocytes.

Description:

This gene encodes a cell surface adhesion molecule that belongs to a family of adhesion/homing receptors. The encoded protein contains a C-type lectin-like domain, a calcium-binding epidermal growth factor-like domain, and two short complement-like repeats. The gene product is required for binding and subsequent rolling of leucocytes on endothelial cells, facilitating their migration into secondary lymphoid organs and inflammation sites. Single-nucleotide polymorphisms in this gene have been associated with various diseases including immunoglobulin A nephropathy. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

Sequence:

P14151 LYAM1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MIFPWKCQSTQRDLWNIFKLWGWTMLCCDFLAHHGTDCWTYHYSEKPMNWQRARRFCRDNYTDLVAIQNKAEIEYLEKTLPFSRSYYWIGIRKIGGIWTWVGTNKSLTEEAENWGDGEPNNKKNKEDCVEIYIKRNKDAGKWNDDACHKLKAALCYTASCQPWSCSGHGECVEIINNYTCNCDVGYYGPQCQFVIQCEPLEAPELGTMDCTHPLGNFSFSSQCAFSCSEGTNLTGIEETTCGPFGNWSSPEPTCQVIQCEPLSAPDLGIMNCSHPLASFSFTSACTFICSEGTELIGKKKTICESSGIWSNPSPICQKLDKSFSMIKEGDYNPLFIPVAVMVTAFSGLAFIIWLARRLKKGKKSKRSMNDPY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Dog

100

Bovine

Rabbit

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P14151 As Substrate

P14151

Site	PTM Type	Source
N60	N-Glycosylation	Uniprot
K78	Ubiquitination	Uniprot
K93	Ubiquitination	Uniprot
N104	N-Glycosylation	Uniprot
K105	Ubiquitination	Uniprot
K122	Ubiquitination	Uniprot
K125	Ubiquitination	Uniprot
Y132	Phosphorylation	Uniprot
K134	Ubiquitination	Uniprot
K137	Ubiquitination	Uniprot
K141	Ubiquitination	Uniprot
K149	Ubiquitination	Uniprot
N177	N-Glycosylation	Uniprot
K300	Ubiquitination	Uniprot
K318	Ubiquitination	Uniprot
K321	Ubiquitination	Uniprot
S364	Phosphorylation	Uniprot
S367	Phosphorylation	Uniprot

Research Backgrounds

P14151_LYAM1_HUMAN

Function:

Calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells. Mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes. Promotes initial tethering and rolling of leukocytes in endothelia.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Expressed in B-cell lines and T-lymphocytes.

Subunit Structure:

Interaction with SELPLG/PSGL1 and PODXL2 is required for promoting recruitment and rolling of leukocytes. This interaction is dependent on the sialyl Lewis X glycan modification of SELPLG and PODXL2, and tyrosine sulfation modifications of SELPLG. Sulfation on 'Tyr-51' of SELPLG is important for L-selectin binding.

Family&Domains:

Belongs to the selectin/LECAM family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

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SELL Antibody - #DF6509