IL23R Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat brain, using IL23R Antibody at 1/1000 dilution.

Product:	IL23R Antibody
Catalog:	DF6501
Description:	Rabbit polyclonal antibody to IL23R
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	69kDa; 72kD(Calculated).
Uniprot:	Q5VWK5
RRID:	AB_2838463

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Bovine(82%), Horse(88%), Sheep(88%), Rabbit(80%), Dog(94%)

Clonality:

Polyclonal

Specificity:

IL23R Antibody detects endogenous levels of total IL23R.

RRID:

AB_2838463
Cite Format: Affinity Biosciences Cat# DF6501, RRID:AB_2838463.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

IL 23R; IL-23 receptor; IL-23R; Il23r; IL23R_HUMAN; Interleukin 23 receptor; Interleukin-23 receptor;

Immunogens

Immunogen:

Uniprot:

Q5VWK5(IL23R_HUMAN) >>Visit HPA database.

Gene(ID):

IL23R(149233)

Expression:

Q5VWK5 IL23R_HUMAN:

Expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells.

Description:

The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner. [provided by RefSeq, Jul 2008]

Sequence:

Q5VWK5 IL23R_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MNQVTIQWDAVIALYILFSWCHGGITNINCSGHIWVEPATIFKMGMNISIYCQAAIKNCQPRKLHFYKNGIKERFQITRINKTTARLWYKNFLEPHASMYCTAECPKHFQETLICGKDISSGYPPDIPDEVTCVIYEYSGNMTCTWNAGKLTYIDTKYVVHVKSLETEEEQQYLTSSYINISTDSLQGGKKYLVWVQAANALGMEESKQLQIHLDDIVIPSAAVISRAETINATVPKTIIYWDSQTTIEKVSCEMRYKATTNQTWNVKEFDTNFTYVQQSEFYLEPNIKYVFQVRCQETGKRYWQPWSSLFFHKTPETVPQVTSKAFQHDTWNSGLTVASISTGHLTSDNRGDIGLLLGMIVFAVMLSILSLIGIFNRSFRTGIKRRILLLIPKWLYEDIPNMKNSNVVKMLQENSELMNNNSSEQVLYVDPMITEIKEIFIPEHKPTDYKKENTGPLETRDYPQNSLFDNTTVVYIPDLNTGYKPQISNFLPEGSHLSNNNEITSLTLKPPVDSLDSGNNPRLQKHPNFAFSVSSVNSLSNTIFLGELSLILNQGECSSPDIQNSVEEETTMLLENDSPSETIPEQTLLPDEFVSCLGIVNEELPSINTYFPQNILESHFNRISLLEK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Pig

Horse

Sheep

Bovine

Rabbit

Zebrafish

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5VWK5 As Substrate

Q5VWK5

Site	PTM Type	Source
N29	N-Glycosylation	Uniprot
N47	N-Glycosylation	Uniprot
Y51	Phosphorylation	Uniprot
N81	N-Glycosylation	Uniprot
T112	Phosphorylation	Uniprot
S120	Phosphorylation	Uniprot
S121	Phosphorylation	Uniprot
N141	N-Glycosylation	Uniprot
S177	Phosphorylation	Uniprot
Y178	Phosphorylation	Uniprot
N180	N-Glycosylation	Uniprot
N232	N-Glycosylation	Uniprot
T234	Phosphorylation	Uniprot
N262	N-Glycosylation	Uniprot
N273	N-Glycosylation	Uniprot
T455	Phosphorylation	Uniprot
T460	Phosphorylation	Uniprot

Research Backgrounds

Q5VWK5_IL23R_HUMAN

Function:

Associates with IL12RB1 to form the interleukin-23 receptor. Binds IL23 and mediates T-cells, NK cells and possibly certain macrophage/myeloid cells stimulation probably through activation of the Jak-Stat signaling cascade. IL23 functions in innate and adaptive immunity and may participate in acute response to infection in peripheral tissues. IL23 may be responsible for autoimmune inflammatory diseases and be important for tumorigenesis.

PTMs:

Phosphorylated in response to IL23.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Tissue Specificity:

Expressed by monocytes, Th1, Th0, NK and dendritic cells. Isoform 1 is specifically expressed in NK cells.

Subunit Structure:

Heterodimer with IL12RB1. In presence of IL23, the heterodimer forms the IL23 receptor. Interacts with JAK2 and in presence of IL23 with STAT3.

Family&Domains:

Belongs to the type I cytokine receptor family. Type 2 subfamily.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Immune diseases > Inflammatory bowel disease (IBD).

· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)

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IL23R Antibody - #DF6501