Product: MUTYH Antibody
Catalog: DF6500
Description: Rabbit polyclonal antibody to MUTYH
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 60kDa; 60kD(Calculated).
Uniprot: Q9UIF7
RRID: AB_2838462

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Sheep(88%), Rabbit(100%), Dog(88%)
Clonality:
Polyclonal
Specificity:
MUTYH Antibody detects endogenous levels of total MUTYH.
RRID:
AB_2838462
Cite Format: Affinity Biosciences Cat# DF6500, RRID:AB_2838462.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

A/G specific adenine DNA glycosylase; A/G-specific adenine DNA glycosylase; Adenine DNA glycosylase; CYP2C; hMYH; MGC4416; MutY (E. coli) homolog; MutY homolog (E. coli); MutY homolog; MutY, E. coli, homolog of; Mutyh; MUTYH_HUMAN; MYH; rMYH;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
Base excision repair (BER) proteins catalyze the removal of incorrect or damaged bases, including oxidized bases, from DNA. N-glycosylases specific to a given lesion remove the incorrect base as the first step in BER. MYH is the mammalian ortholog of E. coli MutY, a DNA glycosylase that catalyzes the removal of 8-oxoG:A mismatches (1). Several MYH isoforms have been detected in human cells localizing to either the nucleus or the mitochondria (2). MYH interacts with DNA repair proteins and localizes to DNA damage foci after oxidative damage (3). Research studies have shown that mutations in the corresponding MYH gene are associated with human gastric (4) and colorectal (5-7) cancers.
Sequence:
MTPLVSRLSRLWAIMRKPRAAVGSGHRKQAASQEGRQKHAKNNSQAKPSACDGMIAECPGAPAGLARQPEEVVLQASVSSYHLFRDVAEVTAFRGSLLSWYDQEKRDLPWRRRAEDEMDLDRRAYAVWVSEVMLQQTQVATVINYYTGWMQKWPTLQDLASASLEEVNQLWAGLGYYSRGRRLQEGARKVVEELGGHMPRTAETLQQLLPGVGRYTAGAIASIAFGQATGVVDGNVARVLCRVRAIGADPSSTLVSQQLWGLAQQLVDPARPGDFNQAAMELGATVCTPQRPLCSQCPVESLCRARQRVEQEQLLASGSLSGSPDVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKASRKPPREESSATCVLEQPGALGAQILLVQRPNSGLLAGLWEFPSVTWEPSEQLQRKALLQELQRWAGPLPATHLRHLGEVVHTFSHIKLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Bovine
88
Sheep
88
Dog
88
Horse
75
Zebrafish
75
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UIF7 As Substrate

Site PTM Type Enzyme
S6 Phosphorylation
S9 Phosphorylation
S24 Phosphorylation
K105 Ubiquitination
K189 Ubiquitination
S360 Phosphorylation
K415 Ubiquitination
Y494 Phosphorylation
S505 Phosphorylation
K506 Ubiquitination
S512 Phosphorylation
S535 Phosphorylation

Research Backgrounds

Function:

Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.

Subcellular Location:

Nucleus. Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the Nth/MutY family.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

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