MUTYH Antibody | Affinity Biosciences

1/2

Western blot analysis of Hela whole cell lysates, using MUTYH Antibody.

DF6500 at 1/100 staining Mouse lung tissue by IHC-P.

Product:	MUTYH Antibody
Catalog:	DF6500
Description:	Rabbit polyclonal antibody to MUTYH
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	60kDa; 60kD(Calculated).
Uniprot:	Q9UIF7
RRID:	AB_2838462

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(88%), Sheep(88%), Rabbit(100%), Dog(88%)

Clonality:

Polyclonal

Specificity:

MUTYH Antibody detects endogenous levels of total MUTYH.

RRID:

AB_2838462
Cite Format: Affinity Biosciences Cat# DF6500, RRID:AB_2838462.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A/G specific adenine DNA glycosylase; A/G-specific adenine DNA glycosylase; Adenine DNA glycosylase; CYP2C; hMYH; MGC4416; MutY (E. coli) homolog; MutY homolog (E. coli); MutY homolog; MutY, E. coli, homolog of; Mutyh; MUTYH_HUMAN; MYH; rMYH;

Immunogens

Immunogen:

Uniprot:

Q9UIF7(MUTYH_HUMAN) >>Visit HPA database.

Gene(ID):

MUTYH(4595)

Description:

Base excision repair (BER) proteins catalyze the removal of incorrect or damaged bases, including oxidized bases, from DNA. N-glycosylases specific to a given lesion remove the incorrect base as the first step in BER. MYH is the mammalian ortholog of E. coli MutY, a DNA glycosylase that catalyzes the removal of 8-oxoG:A mismatches (1). Several MYH isoforms have been detected in human cells localizing to either the nucleus or the mitochondria (2). MYH interacts with DNA repair proteins and localizes to DNA damage foci after oxidative damage (3). Research studies have shown that mutations in the corresponding MYH gene are associated with human gastric (4) and colorectal (5-7) cancers.

Sequence:

Q9UIF7 MUTYH_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MTPLVSRLSRLWAIMRKPRAAVGSGHRKQAASQEGRQKHAKNNSQAKPSACDGMIAECPGAPAGLARQPEEVVLQASVSSYHLFRDVAEVTAFRGSLLSWYDQEKRDLPWRRRAEDEMDLDRRAYAVWVSEVMLQQTQVATVINYYTGWMQKWPTLQDLASASLEEVNQLWAGLGYYSRGRRLQEGARKVVEELGGHMPRTAETLQQLLPGVGRYTAGAIASIAFGQATGVVDGNVARVLCRVRAIGADPSSTLVSQQLWGLAQQLVDPARPGDFNQAAMELGATVCTPQRPLCSQCPVESLCRARQRVEQEQLLASGSLSGSPDVEECAPNTGQCHLCLPPSEPWDQTLGVVNFPRKASRKPPREESSATCVLEQPGALGAQILLVQRPNSGLLAGLWEFPSVTWEPSEQLQRKALLQELQRWAGPLPATHLRHLGEVVHTFSHIKLTYQVYGLALEGQTPVTTVPPGARWLTQEEFHTAAVSTAMKKVFRVYQGQQPGTCMGSKRSQVSSPCSRKKPRMGQQVLDNFFRSHISTDAHSLNSAAQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Bovine

Sheep

Dog

Horse

Zebrafish

Pig

Xenopus

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UIF7 As Substrate

Q9UIF7

Site	PTM Type	Source
S6	Phosphorylation	Uniprot
S9	Phosphorylation	Uniprot
S24	Phosphorylation	Uniprot
K105	Ubiquitination	Uniprot
K189	Ubiquitination	Uniprot
S360	Phosphorylation	Uniprot
K415	Ubiquitination	Uniprot
Y494	Phosphorylation	Uniprot
S505	Phosphorylation	Uniprot
K506	Ubiquitination	Uniprot
S512	Phosphorylation	Uniprot
S535	Phosphorylation	Uniprot

Research Backgrounds

Q9UIF7_MUTYH_HUMAN

Function:

Involved in oxidative DNA damage repair. Initiates repair of A*oxoG to C*G by removing the inappropriately paired adenine base from the DNA backbone. Possesses both adenine and 2-OH-A DNA glycosylase activities.

Subcellular Location:

Nucleus. Mitochondrion.

Family&Domains:

Belongs to the Nth/MutY family.

Research Fields

· Genetic Information Processing > Replication and repair > Base excision repair.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

MUTYH Antibody - #DF6500