Claudin 2 Antibody - #AF0128

Figure 5Commensal Roseburia ameliorates gut-dysbiosis-induced mastitis in mice

Figure 8 MSC-ACE2 upregulates blood-milk barrier-related protein expression to suppress LPS-induced inflammation in Eph4-EV cells. (A) Detection of relative protein expression levels of ZO-1, Claudin-1, Claudin-2 by Western blot; (B) Statistics of the ZO-1, Claudin-1, Claudin-2 Western blot results. Experiments were repeated three times and data were presented as the mean ± SEM (n = 4). * P < 0.05 vs. EpH4-Ev; # P < 0.05 vs. LPS; $ P < 0.05 vs. MSC; & P < 0.05 vs. MSC-GFP.

Fig. 4. Matrine improved gut barrier integrity in DSS-induced UC mice. (A) AB/ PAS and immunofluorescence imaging detected the mucin-producing cells and the expression of mucin-2, respectively. (B) Analysis of the expression levels of the mucin-producing cells and mucin-2. (C) The expressions of occludin, claudin-1,2, and ZO- 1 protein were verified by western blot. (D) Analysis of the expression levels of occludin, claudin-1,2, and ZO-1. Data are expressed as means ± SEM; n = 5 for each group. * P < 0.05, ** P < 0.01, *** P < 0.001, and ****P < 0.0001.

Fig. 4. |Matrine improved gut barrier integrity in DSS-induced UC mice.(C) The expressions of occludin, claudin-1,2, and ZO1 protein were verified by western blot.

Fig. 4 Expression of tight junction-associated proteins and genes and Notch signaling pathway-related proteins and genes in SW480 cells. 1: control group; 2: LPS group; 3: VD3 group; 4: 0.1VC+ VD3 group; 5: 1VC+ VD3 group; 6: 5VC+ VD3 group; 7: 10VC+ VD3 group. a Western blotting results of claudin-2 in SW480 cells, b western blotting results of ZO-1 in SW480 cells, c western blotting results of Notch intracellular domain (NICD) in SW480 cells, d western blotting results of Hes-1 in SW480 cells, e RT-PCR of tight junction protein-related genes and Notch signaling pathway-related genes. In the fgure, a–f indicate signifcant diferences compared with the control group, LPS group, VD3 group, 0.1VC+ VD3 group, 1VC+ VD3 group, and 5VC+ VD3 group, respectively

Fig. 4 Expression of tight junction-associated proteins and genes and Notch signaling pathway-related proteins and genes in SW480 cells. 1: control group; 2: LPS group; 3: VD3 group; 4: 0.1VC+ VD3 group; 5: 1VC+ VD3 group; 6: 5VC+ VD3 group; 7: 10VC+ VD3 group. a Western blotting results of claudin-2 in SW480 cells, b western blotting results of ZO-1 in SW480 cells, c western blotting results of Notch intracellular domain (NICD) in SW480 cells, d western blotting results of Hes-1 in SW480 cells, e RT-PCR of tight junction protein-related genes and Notch signaling pathway-related genes. In the fgure, a–f indicate signifcant diferences compared with the control group, LPS group, VD3 group, 0.1VC+ VD3 group, 1VC+ VD3 group, and 5VC+ VD3 group, respectively

Fig. 1. Cr(VI)-induced tight joint and oxidative damage in the small intestine of mice. Cr(VI)- induced tight joint damage in the small intestine of mice (A–F). After Cr(VI) treatment, Zo- 2 and occludin protein levels were examined via Western blot analysis (A), and the relative ratios of Zo-2 and occludin were quantified in a histogram (B and C). Histopathological changes in the small intestine (D). IHC staining of Zo-1 and claudin-2 (E and F). The first lines of D, E, and F were the respective control groups (I and II), and the second line corresponded to 40 mg/kg Cr groups (III and IV). Higher magnification images of the outlined area are shown on the right (II and IV). Cr(VI)-induced oxidative damage in the small intestine of mice (G–I). Small intestinal homogenate samples were collected to examine the levels of SOD (G), MDA (H), and GSH-Px (I). Values in the same column with different superscripts (a, b, c, d) significantly differed and represented as mean ± SD. Differences with p < 0.05 were presented. All experimental data were obtained from three independent experiments.

Figure 4. |Effects of CB on the expression levels of claudin‑1, claudin‑2, occludin and ZO‑1. (A) Protein expression levels of claudin‑1, claudin‑2, occludin and ZO‑1 in each group were measured using western blot analysis.

Figure 1. The improvement of intestinal mucosal permeability. (a) Effects on small intestinal villi; (b) Effects on intestinal tight junction proteins in the intestinal epithelium. C, control group; M, model group; P, positive group; R-l, raw licorice low dose group; R-h, raw licorice high dose group; H-l, honey-roasted licorice low-dose group; H-h, honey-roasted licorice high-dose group.