ELAVL1 Antibody - #DF6496
| Product: | ELAVL1 Antibody |
| Catalog: | DF6496 |
| Description: | Rabbit polyclonal antibody to ELAVL1 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 36kDa; 36kD(Calculated). |
| Uniprot: | Q15717 |
| RRID: | AB_2838458 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF6496, RRID:AB_2838458.
Fold/Unfold
ELAV (embryonic lethal abnormal vision Drosophila) like 1; ELAV (embryonic lethal, abnormal vision, Drosophila) like 1 (Hu antigen R); ELAV like 1; ELAV like RNA binding protein 1; ELAV-like protein 1; ELAV1; ELAV1_HUMAN; Elavl1; Embryonic lethal abnormal vision drosophila homolog like 1; Hu Antigen R; Hu-antigen R; Hua; HuR; MelG;
Immunogens
- Q15717 ELAV1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSNGYEDHMAEDCRGDIGRTNLIVNYLPQNMTQDELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAINTLNGLRLQSKTIKVSYARPSSEVIKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEEAITSFNGHKPPGSSEPITVKFAANPNQNKNVALLSQLYHSPARRFGGPVHHQAQRFRFSPMGVDHMSGLSGVNVPGNASSGWCIFIYNLGQDADEGILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYEEAAMAIASLNGYRLGDKILQVSFKTNKSHK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q15717 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| Y5 | Phosphorylation | Uniprot | |
| R14 | Methylation | Uniprot | |
| Y26 | Phosphorylation | Uniprot | |
| T32 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| S41 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| K50 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| Y63 | Phosphorylation | P52333 (JAK3) | Uniprot |
| Y68 | Phosphorylation | P52333 (JAK3) | Uniprot |
| K72 | Ubiquitination | Uniprot | |
| T80 | Phosphorylation | Uniprot | |
| S88 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K89 | Ubiquitination | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| S100 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K104 | Ubiquitination | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| T118 | Phosphorylation | Q16539 (MAPK14) , O96017 (CHEK2) | Uniprot |
| K120 | Ubiquitination | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| S146 | Phosphorylation | Uniprot | |
| R147 | Methylation | Uniprot | |
| S158 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| K171 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| S197 | Phosphorylation | Uniprot | |
| Y200 | Phosphorylation | P52333 (JAK3) | Uniprot |
| S202 | Phosphorylation | P06493 (CDK1) , Q00535 (CDK5) , P24941 (CDK2) | Uniprot |
| R205 | Methylation | Uniprot | |
| R206 | Methylation | Uniprot | |
| R217 | Methylation | Uniprot | |
| R219 | Methylation | Uniprot | |
| S221 | Phosphorylation | Q05655 (PRKCD) , P17252 (PRKCA) | Uniprot |
| S242 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| S304 | Phosphorylation | O15111 (CHUK) | Uniprot |
| K313 | Ubiquitination | Uniprot | |
| S318 | Phosphorylation | Q05655 (PRKCD) , P17252 (PRKCA) | Uniprot |
| K320 | Acetylation | Uniprot | |
| K320 | Methylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K323 | Ubiquitination | Uniprot | |
| K326 | Ubiquitination | Uniprot |
Research Backgrounds
RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro. With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). Increases the stability of the leptin mRNA harboring an AU-rich element (ARE) in its 3' UTR.
Phosphorylated by MAPKAPK2. Phosphorylated by PRKCD.
Methylated at Arg-217 by CARM1 in macrophages in response to LPS challenge.
Cytoplasm. Nucleus. Cytoplasm>Stress granule.
Note: Translocates into the cytoplasm following phosphorylation by MAPKAPK2 (PubMed:14517288). Likewise, phosphorylation by PRKCD promotes translocation from the nucleus into the cytoplasm, where it is associated with free and cytoskeleton-bound polysomes (PubMed:18285462).Localizes to the stress granules in the presence of PLEKHN1 (By similarity).
Ubiquitous. Detected in brain, liver, thymus and muscle.
Monomer and homodimer (in vitro). Interacts with ANP32A. Interacts with ZNF385A; the interaction is indirect and mRNA-dependent and may regulate p53/TP53 expression (By similarity). Identified in a mRNP complex, at least composed of DHX9, DDX3X, ELAVL1, HNRNPU, IGF2BP1, ILF3, PABPC1, PCBP2, PTBP2, STAU1, STAU2, SYNCRIP and YBX1. Interacts with AGO1 and AGO2. Interacts with IGF2BP2 and IGF2BP3. Interacts with HNRNPL. Interacts with DHX36; this interaction occurs in a RNA-dependent manner. Interacts with ILF3; this interaction occurs in a RNA-dependent manner. Interacts with PLEKHN1. Interacts with SHFL; the interaction increases in presence of RNA. Interacts with YBX1; interaction recruits ELAVL1 on C5-methylcytosine (m5C)-containing mRNAs, thereby promoting mRNA stability.
The first RRM (RNA recognition motif) domain is essential for binding to AU-rich elements.
Belongs to the RRM elav family.
Research Fields
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.