DHFR Antibody - #DF6495

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Product: DHFR Antibody
Catalog: DF6495
Description: Rabbit polyclonal antibody to DHFR
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Chicken
Mol.Wt.: 21kDa; 21kD(Calculated).
Uniprot: P00374
RRID: AB_2838457

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(82%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
DHFR Antibody detects endogenous levels of total DHFR.
RRID:
AB_2838457
Cite Format: Affinity Biosciences Cat# DF6495, RRID:AB_2838457.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DHFR; DHFRP1; Dihydrofolate reductase; DYR; DYR_HUMAN; EC 1.5.1.3;

Immunogens

Immunogen:
Uniprot:

P00374(DYR_HUMAN) >>Visit HPA database.

Gene(ID):
DHFR(1719)
Expression:
P00374 DYR_HUMAN:

Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.

Description:
Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. [provided by RefSeq, Jul 2008]
Sequence:
MVGSLNCIVAVSQNMGIGKNGDLPWPPLRNEFRYFQRMTTTSSVEGKQNLVIMGKKTWFSIPEKNRPLKGRINLVLSRELKEPPQGAHFLSRSLDDALKLTEQPELANKVDMVWIVGGSSVYKEAMNHPGHLKLFVTRIMQDFESDTFFPEIDLEKYKLLPEYPGVLSDVQEEKGIKYKFEVYEKND

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
91
Bovine
91
Sheep
91
Pig
82
Chicken
82
Xenopus
73
Zebrafish
55
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00374 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
K19 Ubiquitination
R29 Methylation
R33 Methylation
K47 Ubiquitination
K55 Ubiquitination
K56 Ubiquitination
K64 Ubiquitination
K69 Ubiquitination
K81 Sumoylation
K81 Ubiquitination
S93 Phosphorylation
K99 Sumoylation
K99 Ubiquitination
K109 Ubiquitination
K133 Ubiquitination
K156 Sumoylation
K156 Ubiquitination
Y157 Phosphorylation
K158 Sumoylation
K158 Ubiquitination
S168 Phosphorylation
K174 Acetylation
K174 Sumoylation
K174 Ubiquitination
K177 Ubiquitination
K179 Sumoylation
K179 Ubiquitination
Y183 Phosphorylation
K185 Ubiquitination

Research Backgrounds

Function:

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

Subcellular Location:

Mitochondrion. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed in fetal and adult tissues, including throughout the fetal and adult brains and whole blood. Expression is higher in the adult brain than in the fetal brain.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the dihydrofolate reductase family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Antifolate resistance.

· Metabolism > Metabolism of cofactors and vitamins > One carbon pool by folate.

· Metabolism > Metabolism of cofactors and vitamins > Folate biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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