HAMP Antibody - #DF6492

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Product: HAMP Antibody
Catalog: DF6492
Description: Rabbit polyclonal antibody to HAMP
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Dog
Mol.Wt.: 9kDa; 9kD(Calculated).
Uniprot: P81172
RRID: AB_2838454

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Dog(86%)
Clonality:
Polyclonal
Specificity:
HAMP Antibody detects endogenous levels of total HAMP.
RRID:
AB_2838454
Cite Format: Affinity Biosciences Cat# DF6492, RRID:AB_2838454.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hamp; HEPC; HEPC_HUMAN; Hepc20; Hepc25; Hepcidin-20; HFE2B; LEAP-1; LEAP1; Liver-expressed antimicrobial peptide 1; PLTR; Putative liver tumor regressor;

Immunogens

Immunogen:
Uniprot:

P81172(HEPC_HUMAN) >>Visit HPA database.

Gene(ID):
HAMP(57817)
Expression:
P81172 HEPC_HUMAN:

Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.

Description:
The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq, Jul 2008]
Sequence:
MALSSQIWAACLLLLLLLASLTSGSVFPQQTGQLAELQPQDRAGARASWMPMFQRRRRRDTHFPICIFCCGCCHRSKCGMCCKT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
86
Horse
75
Bovine
75
Sheep
75
Pig
67
Rabbit
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.

Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.

Family&Domains:

Belongs to the hepcidin family.

References

1). Loss of Lipocalin2 confers cisplatin vulnerability through modulating NF-ĸB mediated ferroptosis via ferroportin. American journal of cancer research, 2024 (PubMed: 38859845) [IF=5.3]
2). High Hepcidin Levels Promote Abnormal Iron Metabolism and Ferroptosis in Chronic Atrophic Gastritis. Biomedicines, 2023 (PubMed: 37760781) [IF=4.7]

Application: IF/ICC    Species: Human    Sample:

Figure 4 Hepcidin levels were elevated in CAG gastric tissue. (A) Hepcidin immunofluorescence staining in the gastric tissue of patients (Scale bar = 100 or 50 μm, n = 3). The bottom images were the larger images of the frame in the top images, and the arrows indicate the location of hepcidin-positive cells. (B) The mean density of hepcidin-positive cells in Panel (A). (C) Double immunofluorescence labeling of H+-K+-ATPase (green color) and hepcidin (red color) in the gastric tissue of CAG rats (Scale bar = 500 or 50 μm, n = 3). The right images (marked 1, 2) were the larger images of the frame in the Merge images. The data are presented as the mean ± SEM. ** p < 0.01 vs. NAG or Con groups. NAG: non-atrophic gastritis patients; CAG: chronic atrophic gastritis patients or rats; Con: the control group of rats.
3). Cardiomyocyte-Specific Overexpression of FPN1 Diminishes Cardiac Hypertrophy Induced by Chronic Intermittent Hypoxia. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2024 [IF=4.5]

Application: IF/ICC    Species: Mouse    Sample: heart tissue

FIGURE 7 Hepcidin-FPN1 involved in the iron deposition during CIH. (A) The hepcidin mRNA levels in heart tissue (n = 6). (B) The immunohistochemical staining of hepcidin protein (scale bar = 75 μm, n = 3). (C) The mean density of hepcidin content is shown in panel B (n = 3). (D) The immunofluorescence double label staining of FPN1 and hepcidin in heart tissue (scale bar = 25 μm, n = 3). The results are presented as the mean ± SEM. **p 
4). Hydrogen Attenuates Chronic Intermittent Hypoxia-Induced Cardiac Hypertrophy by Regulating Iron Metabolism. Current Issues in Molecular Biology, 2023

Application: IF/ICC    Species: Mouse    Sample: cardiac tissue

Figure 5. The Hepcidin level in the cardiac tissue during CIH and the effects on H9C2 cells after IH exposure. (A) The Hepcidin mRNA levels in heart tissue (n = 3). (B,C) The immunohistochemical staining of Hepcidin protein (scale bar = 100 μm, n = 3). (D) The cell viability of H9C2 cells treated with hydrogen for 0, 30, 60, 90, and 120 min (n = 6). (E) The cell viability of H9C2 cells treated with IH and hydrogen for 0, 30, 60, 90, and 120 min (n = 5). (F) The cell viability of H9C2 cells treated with 60 min (n = 6). (G) The ROS level induced by IH (n = 6). (H,I) The fluorescence intensity of Fe2+ (scale bar = 100 μm, n = 3). (J) The immunofluorescence double-label staining of FPN1 and Hepcidin (scale bar = 100 μm, n = 3). The results are presented as the mean ± SEM. * p < 0.05, ** p < 0.01 vs. Con group. # p < 0.05, ## p < 0.01 vs. IH group.

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